##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BciVI BclI BglII BlnI BsaAI
BsaBI BsaHI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI DraIII DrdI Eam1105I
Eco47III Eco81I EcoRI EcoRV FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MaeII MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI SacI SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 2237 AAATTT [GA]^AATT[CT]
ApoI 2237 AAATTT [GA]^AATT[CT]
AvaI 297 CCCGGG C^[CT]CG[GA]G
BalI 1390 TGGCCA TGG^CCA
BamHI 270 GGATCC G^GATCC
BanI 1459 GGTGCC G^G[CT][GA]CC
BbsI 329 GTCTTC GAAGAC(2/6)
BcgI 64 GCAGCTGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1577 CTAG C^TAG
BpuAI 329 GTCTTC GAAGAC(2/6)
BsaI 1516 GAGACC GGTCTC(1/5)
BsaWI 14 TCCGGT [AT]^CCGG[AT]
BsmI 2262 GCATTC GAATGC(1/-1)
BsoBI 297 CCCGGG C^[CT]CG[GA]G
BspHI 1826 TCATGA T^CATGA
BspMI 704 GCAGGT ACCTGC(4/8)
BsrBI 324 GAGCGG CCGCTC(-3/-3)
BsrFI 202 GCCGGT [GA]^CCGG[CT]
BstEII 17 GGTCACC G^GT[ACGT]ACC
BstPI 17 GGTCACC G^GT[ACGT]ACC
Cfr10I 202 GCCGGT [GA]^CCGG[CT]
CpoI 222 CGGACCG CG^G[AT]CCG
DraI 2282 TTTAAA TTT^AAA
EcoNI 574 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 17 GGTCACC G^GT[ACGT]ACC
EcoT22I 2260 ATGCAT ATGCA^T
MaeI 1577 CTAG C^TAG
MluNI 1390 TGGCCA TGG^CCA
MscI 1390 TGGCCA TGG^CCA
NcoI 30 CCATGG C^CATGG
NsiI 2260 ATGCAT ATGCA^T
NspI 1204 GCATGT [GA]CATG^[CT]
RcaI 1826 TCATGA T^CATGA
RsrII 222 CGGACCG CG^G[AT]CCG
SapI 1666 GAAGAGC GCTCTTC(1/4)
SmaI 297 CCCGGG CCC^GGG
XmaCI 297 CCCGGG C^CCGGG
XmaI 297 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflII 727 CTTAAG C^TTAAG
1552 CTTAAG
AspHI 189 GAGCAC G[AT]GC[AT]^C
1782 GTGCTC
BfrI 727 CTTAAG C^TTAAG
1552 CTTAAG
BlpI 1530 GCTTAGC GC^T[ACGT]AGC
1922 GCTCAGC
Bpu1102I 1530 GCTTAGC GC^T[ACGT]AGC
1922 GCTCAGC
BsgI 1524 CTGCAC GTGCAG(16/14)
1547 CTGCAC
BsiHKAI 189 GAGCAC G[AT]GC[AT]^C
1782 GTGCTC
BstAPI 519 GCAAGGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2108 GCAAGCACTGC
BstXI 1201 CCAGCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1638 CCACTCTGCTGG
CelII 1530 GCTTAGC GC^T[ACGT]AGC
1922 GCTCAGC
EagI 200 CGGCCG C^GGCCG
319 CGGCCG
EarI 1750 CTCTTC CTCTTC(1/4)
1666 GAAGAG
EclXI 200 CGGCCG C^GGCCG
319 CGGCCG
Eco52I 200 CGGCCG C^GGCCG
319 CGGCCG
HaeII 76 GGCGCT [GA]GCGC^[CT]
162 AGCGCC
Ksp632I 1750 CTCTTC CTCTTC(1/4)
1666 GAAGAG
KspI 138 CCGCGG CCGC^GG
154 CCGCGG
PvuII 6 CAGCTG CAG^CTG
65 CAGCTG
SacII 138 CCGCGG CCGC^GG
154 CCGCGG
SfiI 311 GGCCCTTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
391 GGCCGCCTCGGCC
StuI 58 AGGCCT AGG^CCT
946 AGGCCT
TaqI 363 TCGA T^CGA
466 TCGA
Tsp45I 18 GTCAC ^GT[GC]AC
1489 GTCAC
TthHB8I 363 TCGA T^CGA
466 TCGA
XcmI 244 CCAGTCGGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1029 CCAGTTCTCGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaII BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI Tru9I TseI
Tsp509I TspRI Van91I XhoII