##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AviII
AvrII BbeI BbrPI BciVI BglII
BlnI BsaBI BsaI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspMI BsrGI Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI SspBI SspI
SwaI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 1007 GTCTAC GT^[AC][GT]AC
AccII 431 CGCG CG^CG
AclI 598 AACGTT AA^CGTT
AcyI 2444 GGCGTC G[GA]^CG[CT]C
AflII 646 CTTAAG C^TTAAG
AgeI 91 ACCGGT A^CCGGT
AhdI 1108 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2142 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 917 ATTAAT AT^TAAT
AsnI 917 ATTAAT AT^TAAT
Asp700I 2503 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1108 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 745 GGATCC G^GATCC
BbvCI 1445 CCTCAGC CCTCAGC(-5/-2)
BcgI 1314 CGAAGATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 2435 CCCGG CC^[GC]GG
BfrI 646 CTTAAG C^TTAAG
BglI 1250 GCCGAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1520 TACGTA [CT]AC^GT[GA]
BsaHI 2444 GGCGTC G[GA]^CG[CT]C
BsmBI 2446 CGTCTC CGTCTC(1/5)
BspHI 1287 TCATGA T^CATGA
BsrDI 2108 CATTGC GCAATG(2/0)
BsrFI 91 ACCGGT [GA]^CCGG[CT]
BssHII 430 GCGCGC G^CGCGC
BssSI 1775 CTCGTG CACGAG(-5/-1)
BstAPI 1418 GCATGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1401 GGTCACC G^GT[ACGT]ACC
BstPI 1401 GGTCACC G^GT[ACGT]ACC
BstUI 431 CGCG CG^CG
Cfr10I 91 ACCGGT [GA]^CCGG[CT]
Eam1105I 1108 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 961 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1401 GGTCACC G^GT[ACGT]ACC
HaeII 1258 GGCGCT [GA]GCGC^[CT]
Hin1I 2444 GGCGTC G[GA]^CG[CT]C
HincII 524 GTTAAC GT[CT]^[GA]AC
HindII 524 GTTAAC GT[CT]^[GA]AC
HindIII 341 AAGCTT A^AGCTT
HpaI 524 GTTAAC GTT^AAC
MspA1I 1019 CAGCTG C[AC]G^C[GT]G
MvnI 431 CGCG CG^CG
NciI 2435 CCCGG CC^[GC]GG
NheI 1391 GCTAGC G^CTAGC
PinAI 91 ACCGGT A^CCGGT
PshBI 917 ATTAAT AT^TAAT
Psp1406I 598 AACGTT AA^CGTT
PvuII 1019 CAGCTG CAG^CTG
RcaI 1287 TCATGA T^CATGA
SacI 2018 GAGCTC GAGCT^C
SbfI 1819 CCTGCAGG CCTGCA^GG
SnaBI 1520 TACGTA TAC^GTA
SphI 1418 GCATGC GCATG^C
Sse8387I 1819 CCTGCAGG CCTGCA^GG
StuI 805 AGGCCT AGG^CCT
XbaI 724 TCTAGA T^CTAGA
XcmI 1767 CCAGGCCGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2503 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 513 ACATGT A^C[GA][CT]GT
528 ACATGT
AocI 1835 CCTTAGG CC^T[ACGT]AGG
1953 CCTGAGG
AspHI 1758 GAGCAC G[AT]GC[AT]^C
2018 GAGCTC
AspI 383 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1811 GACTCAGTC
BalI 1100 TGGCCA TGG^CCA
1892 TGGCCA
BanI 2052 GGTGCC G^G[CT][GA]CC
2271 GGTGCC
BbsI 181 GTCTTC GAAGAC(2/6)
2210 GTCTTC
BclI 480 TGATCA T^GATCA
700 TGATCA
BlpI 439 GCTGAGC GC^T[ACGT]AGC
2071 GCTGAGC
Bpu1102I 439 GCTGAGC GC^T[ACGT]AGC
2071 GCTGAGC
BpuAI 181 GTCTTC GAAGAC(2/6)
2210 GTCTTC
BsaWI 91 ACCGGT [AT]^CCGG[AT]
471 ACCGGA
BsiHKAI 1758 GAGCAC G[AT]GC[AT]^C
2018 GAGCTC
BspLU11I 513 ACATGT A^CATGT
528 ACATGT
BsrBI 1772 CCGCTC CCGCTC(-3/-3)
1253 GAGCGG
Bsu36I 1835 CCTTAGG CC^T[ACGT]AGG
1953 CCTGAGG
CelII 439 GCTGAGC GC^T[ACGT]AGC
2071 GCTGAGC
DraI 310 TTTAAA TTT^AAA
1408 TTTAAA
DraIII 399 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
986 CACAAAGTG
Eco81I 1835 CCTTAGG CC^T[ACGT]AGG
1953 CCTGAGG
EcoRI 256 GAATTC G^AATTC
572 GAATTC
EcoT14I 2011 CCATGG C^C[AT][AT]GG
2047 CCATGG
FbaI 480 TGATCA T^GATCA
700 TGATCA
HgaI 1388 GACGC GACGC(5/10)
2445 GCGTC
MluNI 1100 TGGCCA TGG^CCA
1892 TGGCCA
MscI 1100 TGGCCA TGG^CCA
1892 TGGCCA
MslI 872 CATCACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2512 CATGGAGATG
NcoI 2011 CCATGG C^CATGG
2047 CCATGG
PflFI 383 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1811 GACTCAGTC
PpuMI 2356 AGGACCT [GA]G^G[AT]CC[CT]
2431 AGGACCC
SmlI 646 CTTAAG C^T[CT][GA]AG
2384 CTCAAG
StyI 2011 CCATGG C^C[AT][AT]GG
2047 CCATGG
Tth111I 383 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1811 GACTCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BanII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII