##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AhdI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbeI BbrPI BbvCI BciVI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1312 GTCTAC GT^[AC][GT]AC
AcyI 1291 GGCGTC G[GA]^CG[CT]C
AflIII 2260 ACATGT A^C[GA][CT]GT
AgeI 2278 ACCGGT A^CCGGT
Alw44I 570 GTGCAC G^TGCAC
Aor51HI 1085 AGCGCT AGC^GCT
ApaI 643 GGGCCC GGGCC^C
ApaLI 570 GTGCAC G^TGCAC
AviII 1226 TGCGCA TGC^GCA
BcgI 1806 CGAGGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2456 TGATCA T^GATCA
BglII 1823 AGATCT A^GATCT
BsaHI 1291 GGCGTC G[GA]^CG[CT]C
BsaWI 2278 ACCGGT [AT]^CCGG[AT]
BsgI 1842 CTGCAC GTGCAG(16/14)
BsiEI 1015 CGACCG CG[GA][CT]^CG
BsmBI 2031 GAGACG CGTCTC(1/5)
BspHI 1632 TCATGA T^CATGA
BspLU11I 2260 ACATGT A^CATGT
BspMI 1370 ACCTGC ACCTGC(4/8)
BsrBI 93 CCGCTC CCGCTC(-3/-3)
BsrDI 2382 GCAATG GCAATG(2/0)
BstAPI 394 GCATTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1358 CCAGCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 1180 GAAGAG CTCTTC(1/4)
Eco47III 1085 AGCGCT AGC^GCT
EcoNI 266 CCTCAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2456 TGATCA T^GATCA
FspI 1226 TGCGCA TGC^GCA
Hin1I 1291 GGCGTC G[GA]^CG[CT]C
HincII 1432 GTTGAC GT[CT]^[GA]AC
HindII 1432 GTTGAC GT[CT]^[GA]AC
HindIII 1074 AAGCTT A^AGCTT
Ksp632I 1180 GAAGAG CTCTTC(1/4)
MslI 2213 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 602 GCCGGC GCC^GGC
NgoMIV 602 GCCGGC G^CCGGC
PaeR7I 704 CTCGAG C^TCGAG
PinAI 2278 ACCGGT A^CCGGT
PleI 1111 GACTC GAGTC(4/5)
RcaI 1632 TCATGA T^CATGA
ScaI 2074 AGTACT AGT^ACT
SmaI 16 CCCGGG CCC^GGG
SpeI 1188 ACTAGT A^CTAGT
XhoI 704 CTCGAG C^TCGAG
XmaCI 16 CCCGGG C^CCGGG
XmaI 16 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 2203 AAATTT [GA]^AATT[CT]
2565 GAATTT
AocI 266 CCTCAGG CC^T[ACGT]AGG
1886 CCTGAGG
ApoI 2203 AAATTT [GA]^AATT[CT]
2565 GAATTT
AspHI 411 GAGCAC G[AT]GC[AT]^C
570 GTGCAC
BglI 936 GCCCCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1720 GCCCTCCAGGC
BsaI 983 GAGACC GGTCTC(1/5)
1707 GGTCTC
BsiHKAI 411 GAGCAC G[AT]GC[AT]^C
570 GTGCAC
BsrFI 602 GCCGGC [GA]^CCGG[CT]
2278 ACCGGT
BssSI 96 CTCGTG CACGAG(-5/-1)
2123 CTCGTG
Bsu36I 266 CCTCAGG CC^T[ACGT]AGG
1886 CCTGAGG
Cfr10I 602 GCCGGC [GA]^CCGG[CT]
2278 ACCGGT
DraI 2199 TTTAAA TTT^AAA
2419 TTTAAA
Eco81I 266 CCTCAGG CC^T[ACGT]AGG
1886 CCTGAGG
HgaI 2109 GACGC GACGC(5/10)
1292 GCGTC
NcoI 1515 CCATGG C^CATGG
2235 CCATGG
PflMI 1347 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2298 CCAGGAACTGG
PstI 401 CTGCAG CTGCA^G
1202 CTGCAG
PvuII 2019 CAGCTG CAG^CTG
2430 CAGCTG
StuI 405 AGGCCT AGG^CCT
888 AGGCCT
TaqI 615 TCGA T^CGA
705 TCGA
TthHB8I 615 TCGA T^CGA
705 TCGA
Van91I 1347 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2298 CCAGGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PpuMI PshAI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII