##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccIII AclI AcsI AflIII
AgeI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AspI AvrII
BamHI BbrPI BbsI BcgI BciVI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BstBI
BstEII BstPI CelII ClaI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MroI
MunI NaeI NcoI NgoMIV NotI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tsp509I Tth111I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2189 GGTACC G^GTACC
AccI 2659 GTATAC GT^[AC][GT]AC
AcyI 1583 GGCGCC G[GA]^CG[CT]C
AflII 1093 CTTAAG C^TTAAG
AhdI 1239 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2189 GGTACC G^GTACC
AspEI 1239 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2483 CTCGAG C^[CT]CG[GA]G
BbeI 1583 GGCGCC GGCGC^C
BbvCI 29 GCTGAGG CCTCAGC(-5/-2)
BclI 2547 TGATCA T^GATCA
BfrI 1093 CTTAAG C^TTAAG
BsaAI 1558 TACGTG [CT]AC^GT[GA]
BsaHI 1583 GGCGCC G[GA]^CG[CT]C
BsoBI 2483 CTCGAG C^[CT]CG[GA]G
BsrDI 1773 CATTGC GCAATG(2/0)
BsrFI 1580 ACCGGC [GA]^CCGG[CT]
BssSI 1813 CTCGTG CACGAG(-5/-1)
Bst1107I 2659 GTATAC GTA^TAC
BstZ17I 2659 GTATAC GTA^TAC
Cfr10I 1580 ACCGGC [GA]^CCGG[CT]
DrdI 877 GACCAGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1239 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2547 TGATCA T^GATCA
Hin1I 1583 GGCGCC G[GA]^CG[CT]C
KasI 1583 GGCGCC G^GCGCC
KpnI 2189 GGTACC GGTAC^C
NarI 1583 GGCGCC GG^CGCC
NheI 8 GCTAGC G^CTAGC
NruI 1443 TCGCGA TCG^CGA
PaeR7I 2483 CTCGAG C^TCGAG
PstI 529 CTGCAG CTGCA^G
ScaI 1766 AGTACT AGT^ACT
SfoI 1583 GGCGCC GGC^GCC
SgrAI 1579 CACCGGCG C[GA]^CCGG[CT]G
XhoI 2483 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 40 CGCG CG^CG
1444 CGCG
Alw44I 1003 GTGCAC G^TGCAC
1479 GTGCAC
AlwI 210 GATCC GGATC(4/5)
408 GGATC
AocI 61 CCTCAGG CC^T[ACGT]AGG
2637 CCTGAGG
ApaLI 1003 GTGCAC G^TGCAC
1479 GTGCAC
AviII 689 TGCGCA TGC^GCA
1022 TGCGCA
BalI 2475 TGGCCA TGG^CCA
2606 TGGCCA
BcnI 1587 CCGGG CC^[GC]GG
2037 CCGGG
BsaI 592 GAGACC GGTCTC(1/5)
1237 GAGACC
BstAPI 644 GCAACGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2340 GCAGGACATGC
BstUI 40 CGCG CG^CG
1444 CGCG
BstYI 209 AGATCC [GA]^GATC[CT]
408 GGATCT
Bsu36I 61 CCTCAGG CC^T[ACGT]AGG
2637 CCTGAGG
DsaI 1232 CCGTGG C^C[GA][CT]GG
2580 CCACGG
Eco81I 61 CCTCAGG CC^T[ACGT]AGG
2637 CCTGAGG
FspI 689 TGCGCA TGC^GCA
1022 TGCGCA
HaeII 525 GGCGCT [GA]GCGC^[CT]
1583 GGCGCC
MaeII 363 ACGT A^CGT
1559 ACGT
MflI 209 AGATCC [GA]^GATC[CT]
408 GGATCT
MluNI 2475 TGGCCA TGG^CCA
2606 TGGCCA
MscI 2475 TGGCCA TGG^CCA
2606 TGGCCA
MseI 1094 TTAA T^TAA
2627 TTAA
MvnI 40 CGCG CG^CG
1444 CGCG
NciI 1587 CCGGG CC^[GC]GG
2037 CCGGG
NdeI 195 CATATG CA^TATG
2653 CATATG
PflMI 724 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1284 CCAAACCCTGG
SapI 1102 GAAGAGC GCTCTTC(1/4)
2023 GAAGAGC
SphI 1082 GCATGC GCATG^C
1564 GCATGC
TfiI 238 GAATC G^A[AT]TC
1951 GATTC
Tru9I 1094 TTAA T^TAA
2627 TTAA
Van91I 724 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1284 CCAAACCCTGG
XhoII 209 AGATCC [GA]^GATC[CT]
408 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanI BanII BbvI BfaI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI