##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AclI AflIII AgeI
Alw44I Aor51HI ApaLI AseI AsnI
Asp700I AspI AviII BbeI BbrPI
BciVI BglII BsaAI BsaBI BsaWI
BseAI BsiEI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII KasI
MaeII MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SbfI
SexAI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2419 GGTACC G^GTACC
AcsI 977 AAATTT [GA]^AATT[CT]
AflII 2872 CTTAAG C^TTAAG
ApoI 977 AAATTT [GA]^AATT[CT]
AscI 32 GGCGCGCC GG^CGCGCC
Asp718I 2419 GGTACC G^GTACC
BclI 383 TGATCA T^GATCA
BfrI 2872 CTTAAG C^TTAAG
BsiWI 26 CGTACG C^GTACG
BsmBI 2195 CGTCTC CGTCTC(1/5)
BsmI 1462 GCATTC GAATGC(1/-1)
BssHII 33 GCGCGC G^CGCGC
BstBI 504 TTCGAA TT^CGAA
FbaI 383 TGATCA T^GATCA
HpaI 1410 GTTAAC GTT^AAC
KpnI 2419 GGTACC GGTAC^C
KspI 659 CCGCGG CCGC^GG
NaeI 8 GCCGGC GCC^GGC
NgoMIV 8 GCCGGC G^CCGGC
NspV 504 TTCGAA TT^CGAA
SacI 2567 GAGCTC GAGCT^C
SacII 659 CCGCGG CCGC^GG
SalI 2129 GTCGAC G^TCGAC
ScaI 2219 AGTACT AGT^ACT
SfiI 699 GGCCTCCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 504 TTCGAA TT^CGAA
SmaI 2508 CCCGGG CCC^GGG
XmaCI 2508 CCCGGG C^CCGGG
XmaI 2508 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AhdI 908 GACTAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1802 GACATTCGGTC
ApaI 354 GGGCCC GGGCC^C
2909 GGGCCC
AspEI 908 GACTAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1802 GACATTCGGTC
BamHI 203 GGATCC G^GATCC
2599 GGATCC
BcgI 1436 CGACAATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2143 GCAGGAGCTTCG
BlpI 2138 GCTGAGC GC^T[ACGT]AGC
2673 GCTAAGC
Bpu1102I 2138 GCTGAGC GC^T[ACGT]AGC
2673 GCTAAGC
BstAPI 1857 GCAAGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3049 GCACCAAGTGC
CelII 2138 GCTGAGC GC^T[ACGT]AGC
2673 GCTAAGC
DraIII 1344 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3050 CACCAAGTG
Eam1105I 908 GACTAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1802 GACATTCGGTC
HaeII 407 AGCGCC [GA]GCGC^[CT]
652 AGCGCC
HincII 1410 GTTAAC GT[CT]^[GA]AC
2129 GTCGAC
HindII 1410 GTTAAC GT[CT]^[GA]AC
2129 GTCGAC
PaeR7I 248 CTCGAG C^TCGAG
294 CTCGAG
PvuII 1867 CAGCTG CAG^CTG
2532 CAGCTG
SapI 2049 GCTCTTC GCTCTTC(1/4)
2095 GCTCTTC
Tsp509I 634 AATT ^AATT
978 AATT
XhoI 248 CTCGAG C^TCGAG
294 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlnI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII