##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI ApoI AscI AseI AsnI
AspI AviII BbrPI BciVI BclI
BfrI BsiWI BsmI BspDI BspHI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KspI
MfeI MluI MunI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI Sse8387I SspI SwaI
Tth111I XcmI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2982 GGTACC G^GTACC
Alw44I 2673 GTGCAC G^TGCAC
ApaI 1587 GGGCCC GGGCC^C
ApaLI 2673 GTGCAC G^TGCAC
Asp700I 409 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2982 GGTACC G^GTACC
BcgI 2281 GCAGGACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2008 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3950 AGATCT A^GATCT
BsmBI 1874 CGTCTC CGTCTC(1/5)
Bsp1407I 4418 TGTACA T^GTACA
BsrBI 2892 GAGCGG CCGCTC(-3/-3)
BsrDI 3756 CATTGC GCAATG(2/0)
BsrFI 4034 GCCGGC [GA]^CCGG[CT]
BsrGI 4418 TGTACA T^GTACA
BssSI 3398 CTCGTG CACGAG(-5/-1)
BstAPI 1169 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 4034 GCCGGC [GA]^CCGG[CT]
DrdI 2506 GACCCCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 4515 GTTGAC GT[CT]^[GA]AC
HindII 4515 GTTGAC GT[CT]^[GA]AC
KpnI 2982 GGTACC GGTAC^C
NaeI 4034 GCCGGC GCC^GGC
NgoMIV 4034 GCCGGC G^CCGGC
NspI 1484 GCATGC [GA]CATG^[CT]
PaeR7I 2289 CTCGAG C^TCGAG
PvuI 3956 CGATCG CGAT^CG
SacI 2167 GAGCTC GAGCT^C
SmaI 2510 CCCGGG CCC^GGG
SpeI 3310 ACTAGT A^CTAGT
SphI 1484 GCATGC GCATG^C
SspBI 4418 TGTACA T^GTACA
StuI 1840 AGGCCT AGG^CCT
XbaI 198 TCTAGA T^CTAGA
XhoI 2289 CTCGAG C^TCGAG
XmaCI 2510 CCCGGG C^CCGGG
XmaI 2510 CCCGGG C^CCGGG
XmnI 409 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 3251 GTAGAC GT^[AC][GT]AC
3841 GTCTAC
AccIII 2542 TCCGGA T^CCGGA
3493 TCCGGA
Aor51HI 2873 AGCGCT AGC^GCT
3821 AGCGCT
AvaI 2289 CTCGAG C^[CT]CG[GA]G
2510 CCCGGG
AvrII 1522 CCTAGG C^CTAGG
1915 CCTAGG
BbeI 782 GGCGCC GGCGC^C
3420 GGCGCC
BlnI 1522 CCTAGG C^CTAGG
1915 CCTAGG
BsaAI 2732 TACGTG [CT]AC^GT[GA]
3944 CACGTA
BsaBI 1664 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3951 GATCTCGATC
BseAI 2542 TCCGGA T^CCGGA
3493 TCCGGA
BsoBI 2289 CTCGAG C^[CT]CG[GA]G
2510 CCCGGG
BspEI 2542 TCCGGA T^CCGGA
3493 TCCGGA
EagI 2336 CGGCCG C^GGCCG
4032 CGGCCG
EclXI 2336 CGGCCG C^GGCCG
4032 CGGCCG
Eco47III 2873 AGCGCT AGC^GCT
3821 AGCGCT
Eco52I 2336 CGGCCG C^GGCCG
4032 CGGCCG
KasI 782 GGCGCC G^GCGCC
3420 GGCGCC
MamI 1664 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3951 GATCTCGATC
MroI 2542 TCCGGA T^CCGGA
3493 TCCGGA
NarI 782 GGCGCC GG^CGCC
3420 GGCGCC
NheI 2102 GCTAGC G^CTAGC
4367 GCTAGC
PflMI 39 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4160 CCACAAGGTGG
SapI 1753 GAAGAGC GCTCTTC(1/4)
2607 GAAGAGC
SfoI 782 GGCGCC GGC^GCC
3420 GGCGCC
Van91I 39 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4160 CCACAAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AocI AspEI
AspHI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII