################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AclI AcsI AflII AflIII AgeI ApoI AscI AseI AsnI AspI AviII BbrPI BciVI BclI BfrI BsiWI BsmI BspDI BspHI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KspI MfeI MluI MunI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SspI SwaI Tth111I XcmI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 2982 GGTACC G^GTACC Alw44I 2673 GTGCAC G^TGCAC ApaI 1587 GGGCCC GGGCC^C ApaLI 2673 GTGCAC G^TGCAC Asp700I 409 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2982 GGTACC G^GTACC BcgI 2281 GCAGGACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 2008 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3950 AGATCT A^GATCT BsmBI 1874 CGTCTC CGTCTC(1/5) Bsp1407I 4418 TGTACA T^GTACA BsrBI 2892 GAGCGG CCGCTC(-3/-3) BsrDI 3756 CATTGC GCAATG(2/0) BsrFI 4034 GCCGGC [GA]^CCGG[CT] BsrGI 4418 TGTACA T^GTACA BssSI 3398 CTCGTG CACGAG(-5/-1) BstAPI 1169 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 4034 GCCGGC [GA]^CCGG[CT] DrdI 2506 GACCCCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HincII 4515 GTTGAC GT[CT]^[GA]AC HindII 4515 GTTGAC GT[CT]^[GA]AC KpnI 2982 GGTACC GGTAC^C NaeI 4034 GCCGGC GCC^GGC NgoMIV 4034 GCCGGC G^CCGGC NspI 1484 GCATGC [GA]CATG^[CT] PaeR7I 2289 CTCGAG C^TCGAG PvuI 3956 CGATCG CGAT^CG SacI 2167 GAGCTC GAGCT^C SmaI 2510 CCCGGG CCC^GGG SpeI 3310 ACTAGT A^CTAGT SphI 1484 GCATGC GCATG^C SspBI 4418 TGTACA T^GTACA StuI 1840 AGGCCT AGG^CCT XbaI 198 TCTAGA T^CTAGA XhoI 2289 CTCGAG C^TCGAG XmaCI 2510 CCCGGG C^CCGGG XmaI 2510 CCCGGG C^CCGGG XmnI 409 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 3251 GTAGAC GT^[AC][GT]AC 3841 GTCTAC AccIII 2542 TCCGGA T^CCGGA 3493 TCCGGA Aor51HI 2873 AGCGCT AGC^GCT 3821 AGCGCT AvaI 2289 CTCGAG C^[CT]CG[GA]G 2510 CCCGGG AvrII 1522 CCTAGG C^CTAGG 1915 CCTAGG BbeI 782 GGCGCC GGCGC^C 3420 GGCGCC BlnI 1522 CCTAGG C^CTAGG 1915 CCTAGG BsaAI 2732 TACGTG [CT]AC^GT[GA] 3944 CACGTA BsaBI 1664 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3951 GATCTCGATC BseAI 2542 TCCGGA T^CCGGA 3493 TCCGGA BsoBI 2289 CTCGAG C^[CT]CG[GA]G 2510 CCCGGG BspEI 2542 TCCGGA T^CCGGA 3493 TCCGGA EagI 2336 CGGCCG C^GGCCG 4032 CGGCCG EclXI 2336 CGGCCG C^GGCCG 4032 CGGCCG Eco47III 2873 AGCGCT AGC^GCT 3821 AGCGCT Eco52I 2336 CGGCCG C^GGCCG 4032 CGGCCG KasI 782 GGCGCC G^GCGCC 3420 GGCGCC MamI 1664 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3951 GATCTCGATC MroI 2542 TCCGGA T^CCGGA 3493 TCCGGA NarI 782 GGCGCC GG^CGCC 3420 GGCGCC NheI 2102 GCTAGC G^CTAGC 4367 GCTAGC PflMI 39 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4160 CCACAAGGTGG SapI 1753 GAAGAGC GCTCTTC(1/4) 2607 GAAGAGC SfoI 782 GGCGCC GGC^GCC 3420 GGCGCC Van91I 39 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4160 CCACAAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcyI AfaI AhdI AluI AlwI AlwNI AocI AspEI AspHI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII