##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII BamHI BcgI BclI BglI
BsaBI BseAI BsiWI BspEI BspLU11I
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI KpnI
MamI MfeI MluI MroI MunI
NheI NotI NruI NspV PaeR7I
PinAI PmeI PshBI PvuI RsrII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1744 GACGTC GACGT^C
AclI 2359 AACGTT AA^CGTT
AcsI 2896 AAATTC [GA]^AATT[CT]
AflII 3564 CTTAAG C^TTAAG
AflIII 2757 ACGTGT A^C[GA][CT]GT
Aor51HI 3986 AGCGCT AGC^GCT
ApaI 3607 GGGCCC GGGCC^C
ApoI 2896 AAATTC [GA]^AATT[CT]
AspI 3370 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 18 CCTAGG C^CTAGG
BbrPI 2756 CACGTG CAC^GTG
BciVI 1571 GGATAC GTATCC(6/5)
BfrI 3564 CTTAAG C^TTAAG
BglII 1206 AGATCT A^GATCT
BlnI 18 CCTAGG C^CTAGG
BsaAI 2756 CACGTG [CT]AC^GT[GA]
BsmBI 2549 GAGACG CGTCTC(1/5)
Bsp1407I 360 TGTACA T^GTACA
BspDI 2240 ATCGAT AT^CGAT
BspHI 3279 TCATGA T^CATGA
BsrDI 877 GCAATG GCAATG(2/0)
BsrGI 360 TGTACA T^GTACA
BssSI 845 CACGAG CACGAG(-5/-1)
BstXI 58 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2240 ATCGAT AT^CGAT
DrdI 271 GACCCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3986 AGCGCT AGC^GCT
EcoNI 907 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 2280 AAGCTT A^AGCTT
HpaI 2816 GTTAAC GTT^AAC
NaeI 804 GCCGGC GCC^GGC
NdeI 3205 CATATG CA^TATG
NgoMIV 804 GCCGGC G^CCGGC
PacI 3225 TTAATTAA TTAAT^TAA
PflFI 3370 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2756 CACGTG CAC^GTG
PmlI 2756 CACGTG CAC^GTG
PshAI 1729 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2359 AACGTT AA^CGTT
PstI 1418 CTGCAG CTGCA^G
RcaI 3279 TCATGA T^CATGA
SacI 2718 GAGCTC GAGCT^C
SapI 2601 GAAGAGC GCTCTTC(1/4)
ScaI 1254 AGTACT AGT^ACT
SspBI 360 TGTACA T^GTACA
Tth111I 3370 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2998 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3830 GTAGAC GT^[AC][GT]AC
4130 GTCTAC
Alw44I 1642 GTGCAC G^TGCAC
3072 GTGCAC
AocI 2514 CCTAAGG CC^T[ACGT]AGG
3659 CCTCAGG
ApaLI 1642 GTGCAC G^TGCAC
3072 GTGCAC
BalI 55 TGGCCA TGG^CCA
67 TGGCCA
BbsI 710 GTCTTC GAAGAC(2/6)
3292 GTCTTC
BbvCI 2574 CCTCAGC CCTCAGC(-5/-2)
2612 CCTCAGC
BlpI 811 GCTGAGC GC^T[ACGT]AGC
1145 GCTCAGC
Bpu1102I 811 GCTGAGC GC^T[ACGT]AGC
1145 GCTCAGC
BpuAI 710 GTCTTC GAAGAC(2/6)
3292 GTCTTC
BsaI 826 GGTCTC GGTCTC(1/5)
3477 GGTCTC
BsaWI 1917 TCCGGT [AT]^CCGG[AT]
2476 TCCGGT
BsmI 3268 GAATGC GAATGC(1/-1)
1945 GCATTC
BsrBI 1841 CCGCTC CCGCTC(-3/-3)
2733 CCGCTC
BstYI 1062 AGATCC [GA]^GATC[CT]
1206 AGATCT
Bsu36I 2514 CCTAAGG CC^T[ACGT]AGG
3659 CCTCAGG
CelII 811 GCTGAGC GC^T[ACGT]AGC
1145 GCTCAGC
EaeI 55 TGGCCA [CT]^GGCC[GA]
67 TGGCCA
Eco81I 2514 CCTAAGG CC^T[ACGT]AGG
3659 CCTCAGG
EcoT22I 4291 ATGCAT ATGCA^T
4307 ATGCAT
KspI 577 CCGCGG CCGC^GG
639 CCGCGG
MflI 1062 AGATCC [GA]^GATC[CT]
1206 AGATCT
MluNI 55 TGGCCA TGG^CCA
67 TGGCCA
MscI 55 TGGCCA TGG^CCA
67 TGGCCA
NsiI 4291 ATGCAT ATGCA^T
4307 ATGCAT
NspI 864 GCATGT [GA]CATG^[CT]
3360 ACATGC
PflMI 844 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2219 CCAGCATTTGG
SacII 577 CCGCGG CCGC^GG
639 CCGCGG
StuI 1089 AGGCCT AGG^CCT
1692 AGGCCT
Van91I 844 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2219 CCAGCATTTGG
XhoII 1062 AGATCC [GA]^GATC[CT]
1206 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmaCI
XmaI