################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## Acc65I AccIII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AviII BamHI BcgI BclI BglI BsaBI BseAI BsiWI BspEI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI KpnI MamI MfeI MluI MroI MunI NheI NotI NruI NspV PaeR7I PinAI PmeI PshBI PvuI RsrII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1744 GACGTC GACGT^C AclI 2359 AACGTT AA^CGTT AcsI 2896 AAATTC [GA]^AATT[CT] AflII 3564 CTTAAG C^TTAAG AflIII 2757 ACGTGT A^C[GA][CT]GT Aor51HI 3986 AGCGCT AGC^GCT ApaI 3607 GGGCCC GGGCC^C ApoI 2896 AAATTC [GA]^AATT[CT] AspI 3370 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 18 CCTAGG C^CTAGG BbrPI 2756 CACGTG CAC^GTG BciVI 1571 GGATAC GTATCC(6/5) BfrI 3564 CTTAAG C^TTAAG BglII 1206 AGATCT A^GATCT BlnI 18 CCTAGG C^CTAGG BsaAI 2756 CACGTG [CT]AC^GT[GA] BsmBI 2549 GAGACG CGTCTC(1/5) Bsp1407I 360 TGTACA T^GTACA BspDI 2240 ATCGAT AT^CGAT BspHI 3279 TCATGA T^CATGA BsrDI 877 GCAATG GCAATG(2/0) BsrGI 360 TGTACA T^GTACA BssSI 845 CACGAG CACGAG(-5/-1) BstXI 58 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 2240 ATCGAT AT^CGAT DrdI 271 GACCCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3986 AGCGCT AGC^GCT EcoNI 907 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HindIII 2280 AAGCTT A^AGCTT HpaI 2816 GTTAAC GTT^AAC NaeI 804 GCCGGC GCC^GGC NdeI 3205 CATATG CA^TATG NgoMIV 804 GCCGGC G^CCGGC PacI 3225 TTAATTAA TTAAT^TAA PflFI 3370 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2756 CACGTG CAC^GTG PmlI 2756 CACGTG CAC^GTG PshAI 1729 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2359 AACGTT AA^CGTT PstI 1418 CTGCAG CTGCA^G RcaI 3279 TCATGA T^CATGA SacI 2718 GAGCTC GAGCT^C SapI 2601 GAAGAGC GCTCTTC(1/4) ScaI 1254 AGTACT AGT^ACT SspBI 360 TGTACA T^GTACA Tth111I 3370 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2998 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 3830 GTAGAC GT^[AC][GT]AC 4130 GTCTAC Alw44I 1642 GTGCAC G^TGCAC 3072 GTGCAC AocI 2514 CCTAAGG CC^T[ACGT]AGG 3659 CCTCAGG ApaLI 1642 GTGCAC G^TGCAC 3072 GTGCAC BalI 55 TGGCCA TGG^CCA 67 TGGCCA BbsI 710 GTCTTC GAAGAC(2/6) 3292 GTCTTC BbvCI 2574 CCTCAGC CCTCAGC(-5/-2) 2612 CCTCAGC BlpI 811 GCTGAGC GC^T[ACGT]AGC 1145 GCTCAGC Bpu1102I 811 GCTGAGC GC^T[ACGT]AGC 1145 GCTCAGC BpuAI 710 GTCTTC GAAGAC(2/6) 3292 GTCTTC BsaI 826 GGTCTC GGTCTC(1/5) 3477 GGTCTC BsaWI 1917 TCCGGT [AT]^CCGG[AT] 2476 TCCGGT BsmI 3268 GAATGC GAATGC(1/-1) 1945 GCATTC BsrBI 1841 CCGCTC CCGCTC(-3/-3) 2733 CCGCTC BstYI 1062 AGATCC [GA]^GATC[CT] 1206 AGATCT Bsu36I 2514 CCTAAGG CC^T[ACGT]AGG 3659 CCTCAGG CelII 811 GCTGAGC GC^T[ACGT]AGC 1145 GCTCAGC EaeI 55 TGGCCA [CT]^GGCC[GA] 67 TGGCCA Eco81I 2514 CCTAAGG CC^T[ACGT]AGG 3659 CCTCAGG EcoT22I 4291 ATGCAT ATGCA^T 4307 ATGCAT KspI 577 CCGCGG CCGC^GG 639 CCGCGG MflI 1062 AGATCC [GA]^GATC[CT] 1206 AGATCT MluNI 55 TGGCCA TGG^CCA 67 TGGCCA MscI 55 TGGCCA TGG^CCA 67 TGGCCA NsiI 4291 ATGCAT ATGCA^T 4307 ATGCAT NspI 864 GCATGT [GA]CATG^[CT] 3360 ACATGC PflMI 844 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2219 CCAGCATTTGG SacII 577 CCGCGG CCGC^GG 639 CCGCGG StuI 1089 AGGCCT AGG^CCT 1692 AGGCCT Van91I 844 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2219 CCAGCATTTGG XhoII 1062 AGATCC [GA]^GATC[CT] 1206 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmaCI XmaI