##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII AvrII BcgI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstXI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoRI EcoRV FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NcoI NdeI
NotI NruI PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 2314 GACGTC GACGT^C
AccI 1028 GTAGAC GT^[AC][GT]AC
AflIII 2190 ACGTGT A^C[GA][CT]GT
Alw44I 1532 GTGCAC G^TGCAC
AocI 2932 CCTTAGG CC^T[ACGT]AGG
ApaLI 1532 GTGCAC G^TGCAC
Asp700I 1432 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 42 GGATCC G^GATCC
BbeI 1570 GGCGCC GGCGC^C
BbrPI 2189 CACGTG CAC^GTG
BciVI 2161 GGATAC GTATCC(6/5)
BlpI 2287 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2287 GCTCAGC GC^T[ACGT]AGC
BsaAI 2189 CACGTG [CT]AC^GT[GA]
BsiEI 2474 CGGCCG CG[GA][CT]^CG
BsmBI 2243 CGTCTC CGTCTC(1/5)
BsrDI 3127 CATTGC GCAATG(2/0)
BssSI 2836 CTCGTG CACGAG(-5/-1)
BstBI 732 TTCGAA TT^CGAA
Bsu36I 2932 CCTTAGG CC^T[ACGT]AGG
CelII 2287 GCTCAGC GC^T[ACGT]AGC
EagI 2474 CGGCCG C^GGCCG
EclXI 2474 CGGCCG C^GGCCG
Eco52I 2474 CGGCCG C^GGCCG
Eco81I 2932 CCTTAGG CC^T[ACGT]AGG
EcoNI 2346 CCTACTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 2689 GGCCGGCC GGCCGG^CC
HindIII 1422 AAGCTT A^AGCTT
KasI 1570 GGCGCC G^GCGCC
NaeI 2690 GCCGGC GCC^GGC
NarI 1570 GGCGCC GG^CGCC
NgoMIV 2690 GCCGGC G^CCGGC
NheI 469 GCTAGC G^CTAGC
NspI 635 GCATGC [GA]CATG^[CT]
NspV 732 TTCGAA TT^CGAA
PflMI 3322 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2189 CACGTG CAC^GTG
PmlI 2189 CACGTG CAC^GTG
PshAI 3117 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2285 GAGCTC GAGCT^C
SfoI 1570 GGCGCC GGC^GCC
SfuI 732 TTCGAA TT^CGAA
SmaI 66 CCCGGG CCC^GGG
SphI 635 GCATGC GCATG^C
TfiI 1300 GAATC G^A[AT]TC
Van91I 3322 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3190 TCTAGA T^CTAGA
XmaCI 66 CCCGGG C^CCGGG
XmaI 66 CCCGGG C^CCGGG
XmnI 1432 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 331 AAATTC [GA]^AATT[CT]
1400 AAATTC
AcyI 1570 GGCGCC G[GA]^CG[CT]C
2314 GACGTC
ApoI 331 AAATTC [GA]^AATT[CT]
1400 AAATTC
AvaI 66 CCCGGG C^[CT]CG[GA]G
1574 CCCGAG
BalI 763 TGGCCA TGG^CCA
1338 TGGCCA
BclI 1555 TGATCA T^GATCA
3208 TGATCA
BsaHI 1570 GGCGCC G[GA]^CG[CT]C
2314 GACGTC
BsmI 872 GAATGC GAATGC(1/-1)
805 GCATTC
BsoBI 66 CCCGGG C^[CT]CG[GA]G
1574 CCCGAG
BspHI 1924 TCATGA T^CATGA
3844 TCATGA
BsrBI 743 GAGCGG CCGCTC(-3/-3)
2486 GAGCGG
BstAPI 1983 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2066 GCAAAGTATGC
BstEII 2889 GGTGACC G^GT[ACGT]ACC
2986 GGTCACC
BstPI 2889 GGTGACC G^GT[ACGT]ACC
2986 GGTCACC
DraI 442 TTTAAA TTT^AAA
3795 TTTAAA
DsaI 1903 CCACGG C^C[GA][CT]GG
2638 CCGTGG
EcoO65I 2889 GGTGACC G^GT[ACGT]ACC
2986 GGTCACC
EcoT22I 633 ATGCAT ATGCA^T
2208 ATGCAT
FauI 1880 GCGGG CCCGC(4/6)
2488 GCGGG
FbaI 1555 TGATCA T^GATCA
3208 TGATCA
HaeII 726 AGCGCC [GA]GCGC^[CT]
1570 GGCGCC
Hin1I 1570 GGCGCC G[GA]^CG[CT]C
2314 GACGTC
MluNI 763 TGGCCA TGG^CCA
1338 TGGCCA
MscI 763 TGGCCA TGG^CCA
1338 TGGCCA
MslI 919 CATTACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1955 CACCAGTATG
NsiI 633 ATGCAT ATGCA^T
2208 ATGCAT
RcaI 1924 TCATGA T^CATGA
3844 TCATGA
ScaI 1842 AGTACT AGT^ACT
2133 AGTACT
SexAI 1727 ACCAGGT A^CC[AT]GGT
2990 ACCTGGT
SspI 3830 AATATT AAT^ATT
3894 AATATT
StuI 1986 AGGCCT AGG^CCT
2666 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AspI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII