##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BcnI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspMI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI CfoI
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I FauI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI KspI MamI
MluI MroI MspA1I MspI MvnI
NaeI NarI NciI NgoMIV NotI
NruI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 2452 GTATAC GT^[AC][GT]AC
AclI 3457 AACGTT AA^CGTT
AflII 2712 CTTAAG C^TTAAG
AocI 2217 CCTTAGG CC^T[ACGT]AGG
ApaI 4394 GGGCCC GGGCC^C
Asp700I 1956 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 3919 GTGCTC G[AT]GC[AT]^C
BanII 4394 GGGCCC G[GA]GC[CT]^C
BciVI 1020 GGATAC GTATCC(6/5)
BfrI 2712 CTTAAG C^TTAAG
BlpI 1925 GCTGAGC GC^T[ACGT]AGC
BpmI 3175 CTGGAG CTGGAG(16/14)
Bpu1102I 1925 GCTGAGC GC^T[ACGT]AGC
BsgI 535 CTGCAC GTGCAG(16/14)
BsiHKAI 3919 GTGCTC G[AT]GC[AT]^C
BsmBI 1529 GAGACG CGTCTC(1/5)
Bsp1407I 371 TGTACA T^GTACA
BspHI 1357 TCATGA T^CATGA
BsrBI 1619 CCGCTC CCGCTC(-3/-3)
BsrGI 371 TGTACA T^GTACA
Bst1107I 2452 GTATAC GTA^TAC
BstXI 4069 CCAGAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2452 GTATAC GTA^TAC
Bsu36I 2217 CCTTAGG CC^T[ACGT]AGG
CelII 1925 GCTGAGC GC^T[ACGT]AGC
DrdI 1263 GACAAATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 789 CCATGG C^C[GA][CT]GG
Eco81I 2217 CCTTAGG CC^T[ACGT]AGG
EcoRV 2172 GATATC GAT^ATC
EcoT14I 789 CCATGG C^C[AT][AT]GG
EcoT22I 4620 ATGCAT ATGCA^T
HincII 3849 GTTGAC GT[CT]^[GA]AC
HindII 3849 GTTGAC GT[CT]^[GA]AC
HindIII 3656 AAGCTT A^AGCTT
MfeI 3927 CAATTG C^AATTG
MunI 3927 CAATTG C^AATTG
MwoI 1621 GCTCTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 789 CCATGG C^CATGG
NheI 1890 GCTAGC G^CTAGC
NsiI 4620 ATGCAT ATGCA^T
Psp1406I 3457 AACGTT AA^CGTT
RcaI 1357 TCATGA T^CATGA
SpeI 2456 ACTAGT A^CTAGT
SspBI 371 TGTACA T^GTACA
StyI 789 CCATGG C^C[AT][AT]GG
SwaI 3705 ATTTAAAT ATTT^AAAT
XbaI 1222 TCTAGA T^CTAGA
XmnI 1956 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BalI 1488 TGGCCA TGG^CCA
1861 TGGCCA
BanI 523 GGCACC G^G[CT][GA]CC
2634 GGTGCC
BsaJI 789 CCATGG C^C[ACGT][ACGT]GG
2599 CCCTGG
BsmAI 1529 GAGAC GTCTC(1/5)
2000 GAGAC
BsmI 4529 GAATGC GAATGC(1/-1)
1894 GCATTC
HgaI 1968 GACGC GACGC(5/10)
439 GCGTC
MaeII 3063 ACGT A^CGT
3458 ACGT
MluNI 1488 TGGCCA TGG^CCA
1861 TGGCCA
MscI 1488 TGGCCA TGG^CCA
1861 TGGCCA
PacI 723 TTAATTAA TTAAT^TAA
3252 TTAATTAA
PflMI 163 CCAGTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2018 CCATATTTTGG
PpuMI 4120 GGGTCCC [GA]G^G[AT]CC[CT]
4131 AGGTCCT
SapI 1749 GAAGAGC GCTCTTC(1/4)
2028 GAAGAGC
ScaI 1075 AGTACT AGT^ACT
3027 AGTACT
SfcI 1885 CTGTAG C^T[GA][CT]AG
2659 CTGTAG
TaqI 1319 TCGA T^CGA
1943 TCGA
TthHB8I 1319 TCGA T^CGA
1943 TCGA
Van91I 163 CCAGTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2018 CCATATTTTGG
XcmI 1921 CCAAGCTGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2273 CCAAGCAATTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AvaII
BbvI BclI BfaI BmyI BseRI
BsiYI BslI BsmFI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRI EcoRII FbaI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI NdeI
NdeII NlaIII NlaIV NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII