##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AvaI AvrII BbeI BciVI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspEI BspHI BsrGI BssSI
Bst1107I BstZ17I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoT22I FseI HincII HindII
HindIII HpaI KasI KspI MamI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 678 GACGTC GACGT^C
AccI 1554 GTCTAC GT^[AC][GT]AC
AccII 1042 CGCG CG^CG
AcsI 3143 GAATTT [GA]^AATT[CT]
AcyI 678 GACGTC G[GA]^CG[CT]C
AflIII 1945 ACATGT A^C[GA][CT]GT
Alw44I 534 GTGCAC G^TGCAC
ApaLI 534 GTGCAC G^TGCAC
ApoI 3143 GAATTT [GA]^AATT[CT]
AviII 658 TGCGCA TGC^GCA
BamHI 1729 GGATCC G^GATCC
BbrPI 33 CACGTG CAC^GTG
BbvCI 2494 CCTCAGC CCTCAGC(-5/-2)
BcgI 2838 CGACCTATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2040 TGATCA T^GATCA
BlpI 986 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 986 GCTCAGC GC^T[ACGT]AGC
BsaHI 678 GACGTC G[GA]^CG[CT]C
BspDI 1617 ATCGAT AT^CGAT
BspLU11I 1945 ACATGT A^CATGT
BsrDI 2518 CATTGC GCAATG(2/0)
BsrFI 1429 ACCGGC [GA]^CCGG[CT]
BssHII 1041 GCGCGC G^CGCGC
BstBI 1599 TTCGAA TT^CGAA
BstUI 1042 CGCG CG^CG
CelII 986 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1429 ACCGGC [GA]^CCGG[CT]
ClaI 1617 ATCGAT AT^CGAT
DraIII 558 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 2748 GATATC GAT^ATC
FbaI 2040 TGATCA T^GATCA
FspI 658 TGCGCA TGC^GCA
HgaI 1025 GACGC GACGC(5/10)
Hin1I 678 GACGTC G[GA]^CG[CT]C
MfeI 2875 CAATTG C^AATTG
MunI 2875 CAATTG C^AATTG
MvnI 1042 CGCG CG^CG
NcoI 1696 CCATGG C^CATGG
NspV 1599 TTCGAA TT^CGAA
PmaCI 33 CACGTG CAC^GTG
PmlI 33 CACGTG CAC^GTG
SapI 21 GAAGAGC GCTCTTC(1/4)
SfuI 1599 TTCGAA TT^CGAA
SphI 1457 GCATGC GCATG^C
StuI 9 AGGCCT AGG^CCT
XbaI 3008 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2314 GGTACC G^GTACC
2929 GGTACC
AocI 702 CCTAAGG CC^T[ACGT]AGG
1559 CCTGAGG
ApaI 790 GGGCCC GGGCC^C
1918 GGGCCC
Asp718I 2314 GGTACC G^GTACC
2929 GGTACC
AspHI 534 GTGCAC G[AT]GC[AT]^C
2297 GTGCTC
AspI 1025 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1548 GACCCTGTC
BalI 1376 TGGCCA TGG^CCA
1693 TGGCCA
BsaI 625 GAGACC GGTCTC(1/5)
1546 GAGACC
BsiHKAI 534 GTGCAC G[AT]GC[AT]^C
2297 GTGCTC
BsmI 917 GAATGC GAATGC(1/-1)
1583 GCATTC
BstAPI 1276 GCAAGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1475 GCACCACATGC
BstEII 113 GGTGACC G^GT[ACGT]ACC
2580 GGTAACC
BstPI 113 GGTGACC G^GT[ACGT]ACC
2580 GGTAACC
BstXI 454 CCAGGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2324 CCAGTGCCTTGG
Bsu36I 702 CCTAAGG CC^T[ACGT]AGG
1559 CCTGAGG
DraI 1955 TTTAAA TTT^AAA
3050 TTTAAA
DsaI 1696 CCATGG C^C[GA][CT]GG
2349 CCACGG
EarI 1204 CTCTTC CTCTTC(1/4)
21 GAAGAG
Eco81I 702 CCTAAGG CC^T[ACGT]AGG
1559 CCTGAGG
EcoO65I 113 GGTGACC G^GT[ACGT]ACC
2580 GGTAACC
HaeII 441 AGCGCC [GA]GCGC^[CT]
1521 AGCGCC
KpnI 2314 GGTACC GGTAC^C
2929 GGTACC
Ksp632I 1204 CTCTTC CTCTTC(1/4)
21 GAAGAG
MluNI 1376 TGGCCA TGG^CCA
1693 TGGCCA
MscI 1376 TGGCCA TGG^CCA
1693 TGGCCA
PflFI 1025 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1548 GACCCTGTC
PflMI 1184 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1922 CCAGCTGGTGG
Tth111I 1025 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1548 GACCCTGTC
Van91I 1184 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1922 CCAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII