################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I CelII ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoT22I FbaI FseI FspI HaeII KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspV PacI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcsI 1800 GAATTT [GA]^AATT[CT] AcyI 267 GACGCC G[GA]^CG[CT]C AgeI 1557 ACCGGT A^CCGGT ApoI 1800 GAATTT [GA]^AATT[CT] AspI 1727 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 678 CTCGAG C^[CT]CG[GA]G BalI 492 TGGCCA TGG^CCA BamHI 2355 GGATCC G^GATCC BsaAI 1001 CACGTA [CT]AC^GT[GA] BsaHI 267 GACGCC G[GA]^CG[CT]C BsaI 2348 GAGACC GGTCTC(1/5) BsoBI 678 CTCGAG C^[CT]CG[GA]G BsrBI 1381 CCGCTC CCGCTC(-3/-3) BsrDI 2834 CATTGC GCAATG(2/0) BsrFI 1557 ACCGGT [GA]^CCGG[CT] BstAPI 943 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 2355 GGATCC [GA]^GATC[CT] Cfr10I 1557 ACCGGT [GA]^CCGG[CT] DraIII 1881 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1862 GACTCTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 492 TGGCCA [CT]^GGCC[GA] EcoNI 3104 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 26 GATATC GAT^ATC Hin1I 267 GACGCC G[GA]^CG[CT]C HindIII 35 AAGCTT A^AGCTT MflI 2355 GGATCC [GA]^GATC[CT] MluNI 492 TGGCCA TGG^CCA MscI 492 TGGCCA TGG^CCA MslI 2275 CATGGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NruI 961 TCGCGA TCG^CGA PaeR7I 678 CTCGAG C^TCGAG PflFI 1727 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1557 ACCGGT A^CCGGT SacI 823 GAGCTC GAGCT^C SalI 501 GTCGAC G^TCGAC SexAI 2156 ACCTGGT A^CC[AT]GGT SphI 2547 GCATGC GCATG^C Tth111I 1727 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2976 TCTAGA T^CTAGA XhoI 678 CTCGAG C^TCGAG XhoII 2355 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 501 GTCGAC GT^[AC][GT]AC 3264 GTAGAC AocI 1697 CCTAAGG CC^T[ACGT]AGG 3414 CCTGAGG AspHI 823 GAGCTC G[AT]GC[AT]^C 2270 GTGCTC BanII 655 GAGCCC G[GA]GC[CT]^C 823 GAGCTC BbsI 1321 GAAGAC GAAGAC(2/6) 2779 GAAGAC BpuAI 1321 GAAGAC GAAGAC(2/6) 2779 GAAGAC BsaBI 353 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2066 GATGGACATC BsaWI 261 ACCGGA [AT]^CCGG[AT] 1557 ACCGGT BsiHKAI 823 GAGCTC G[AT]GC[AT]^C 2270 GTGCTC BsmBI 3184 CGTCTC CGTCTC(1/5) 265 GAGACG BstEII 2851 GGTAACC G^GT[ACGT]ACC 3397 GGTTACC BstPI 2851 GGTAACC G^GT[ACGT]ACC 3397 GGTTACC Bsu36I 1697 CCTAAGG CC^T[ACGT]AGG 3414 CCTGAGG CfoI 1026 GCGC GCG^C 1158 GCGC Eco81I 1697 CCTAAGG CC^T[ACGT]AGG 3414 CCTGAGG EcoO65I 2851 GGTAACC G^GT[ACGT]ACC 3397 GGTTACC HgaI 267 GACGC GACGC(5/10) 710 GACGC HhaI 1026 GCGC GCG^C 1158 GCGC HinP1I 1026 GCGC G^CGC 1158 GCGC HpaI 577 GTTAAC GTT^AAC 2020 GTTAAC MamI 353 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2066 GATGGACATC PstI 1667 CTGCAG CTGCA^G 1923 CTGCAG SapI 2318 GAAGAGC GCTCTTC(1/4) 1166 GCTCTTC ScaI 474 AGTACT AGT^ACT 1063 AGTACT SspI 65 AATATT AAT^ATT 2625 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AvaII BbvI BcgI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I