##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BanI        
BbeI        BbrPI       BbvCI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BseAI       BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRI       EcoT22I     FbaI        FseI        FspI        
HaeII       KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NsiI        
NspV        PacI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SbfI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       StuI        SwaI        XcmI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcsI         1800  GAATTT               [GA]^AATT[CT]
AcyI          267  GACGCC               G[GA]^CG[CT]C
AgeI         1557  ACCGGT               A^CCGGT
ApoI         1800  GAATTT               [GA]^AATT[CT]
AspI         1727  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          678  CTCGAG               C^[CT]CG[GA]G
BalI          492  TGGCCA               TGG^CCA
BamHI        2355  GGATCC               G^GATCC
BsaAI        1001  CACGTA               [CT]AC^GT[GA]
BsaHI         267  GACGCC               G[GA]^CG[CT]C
BsaI         2348  GAGACC               GGTCTC(1/5)
BsoBI         678  CTCGAG               C^[CT]CG[GA]G
BsrBI        1381  CCGCTC               CCGCTC(-3/-3)
BsrDI        2834  CATTGC               GCAATG(2/0)
BsrFI        1557  ACCGGT               [GA]^CCGG[CT]
BstAPI        943  GCATCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        2355  GGATCC               [GA]^GATC[CT]
Cfr10I       1557  ACCGGT               [GA]^CCGG[CT]
DraIII       1881  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1862  GACTCTCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          492  TGGCCA               [CT]^GGCC[GA]
EcoNI        3104  CCTGATGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV          26  GATATC               GAT^ATC
Hin1I         267  GACGCC               G[GA]^CG[CT]C
HindIII        35  AAGCTT               A^AGCTT
MflI         2355  GGATCC               [GA]^GATC[CT]
MluNI         492  TGGCCA               TGG^CCA
MscI          492  TGGCCA               TGG^CCA
MslI         2275  CATGGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI          961  TCGCGA               TCG^CGA
PaeR7I        678  CTCGAG               C^TCGAG
PflFI        1727  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1557  ACCGGT               A^CCGGT
SacI          823  GAGCTC               GAGCT^C
SalI          501  GTCGAC               G^TCGAC
SexAI        2156  ACCTGGT              A^CC[AT]GGT
SphI         2547  GCATGC               GCATG^C
Tth111I      1727  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2976  TCTAGA               T^CTAGA
XhoI          678  CTCGAG               C^TCGAG
XhoII        2355  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          501  GTCGAC               GT^[AC][GT]AC
             3264  GTAGAC              
AocI         1697  CCTAAGG              CC^T[ACGT]AGG
             3414  CCTGAGG             
AspHI         823  GAGCTC               G[AT]GC[AT]^C
             2270  GTGCTC              
BanII         655  GAGCCC               G[GA]GC[CT]^C
              823  GAGCTC              
BbsI         1321  GAAGAC               GAAGAC(2/6)
             2779  GAAGAC              
BpuAI        1321  GAAGAC               GAAGAC(2/6)
             2779  GAAGAC              
BsaBI         353  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2066  GATGGACATC          
BsaWI         261  ACCGGA               [AT]^CCGG[AT]
             1557  ACCGGT              
BsiHKAI       823  GAGCTC               G[AT]GC[AT]^C
             2270  GTGCTC              
BsmBI        3184  CGTCTC               CGTCTC(1/5)
              265  GAGACG              
BstEII       2851  GGTAACC              G^GT[ACGT]ACC
             3397  GGTTACC             
BstPI        2851  GGTAACC              G^GT[ACGT]ACC
             3397  GGTTACC             
Bsu36I       1697  CCTAAGG              CC^T[ACGT]AGG
             3414  CCTGAGG             
CfoI         1026  GCGC                 GCG^C
             1158  GCGC                
Eco81I       1697  CCTAAGG              CC^T[ACGT]AGG
             3414  CCTGAGG             
EcoO65I      2851  GGTAACC              G^GT[ACGT]ACC
             3397  GGTTACC             
HgaI          267  GACGC                GACGC(5/10)
              710  GACGC               
HhaI         1026  GCGC                 GCG^C
             1158  GCGC                
HinP1I       1026  GCGC                 G^CGC
             1158  GCGC                
HpaI          577  GTTAAC               GTT^AAC
             2020  GTTAAC              
MamI          353  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2066  GATGGACATC          
PstI         1667  CTGCAG               CTGCA^G
             1923  CTGCAG              
SapI         2318  GAAGAGC              GCTCTTC(1/4)
             1166  GCTCTTC             
ScaI          474  AGTACT               AGT^ACT
             1063  AGTACT              
SspI           65  AATATT               AAT^ATT
             2625  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AvaII       BbvI        BcgI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspHI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I