##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BanI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I CelII ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoT22I FbaI FseI FspI
HaeII KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NsiI
NspV PacI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI XcmI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 1800 GAATTT [GA]^AATT[CT]
AcyI 267 GACGCC G[GA]^CG[CT]C
AgeI 1557 ACCGGT A^CCGGT
ApoI 1800 GAATTT [GA]^AATT[CT]
AspI 1727 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 678 CTCGAG C^[CT]CG[GA]G
BalI 492 TGGCCA TGG^CCA
BamHI 2355 GGATCC G^GATCC
BsaAI 1001 CACGTA [CT]AC^GT[GA]
BsaHI 267 GACGCC G[GA]^CG[CT]C
BsaI 2348 GAGACC GGTCTC(1/5)
BsoBI 678 CTCGAG C^[CT]CG[GA]G
BsrBI 1381 CCGCTC CCGCTC(-3/-3)
BsrDI 2834 CATTGC GCAATG(2/0)
BsrFI 1557 ACCGGT [GA]^CCGG[CT]
BstAPI 943 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 2355 GGATCC [GA]^GATC[CT]
Cfr10I 1557 ACCGGT [GA]^CCGG[CT]
DraIII 1881 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1862 GACTCTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 492 TGGCCA [CT]^GGCC[GA]
EcoNI 3104 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 26 GATATC GAT^ATC
Hin1I 267 GACGCC G[GA]^CG[CT]C
HindIII 35 AAGCTT A^AGCTT
MflI 2355 GGATCC [GA]^GATC[CT]
MluNI 492 TGGCCA TGG^CCA
MscI 492 TGGCCA TGG^CCA
MslI 2275 CATGGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 961 TCGCGA TCG^CGA
PaeR7I 678 CTCGAG C^TCGAG
PflFI 1727 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1557 ACCGGT A^CCGGT
SacI 823 GAGCTC GAGCT^C
SalI 501 GTCGAC G^TCGAC
SexAI 2156 ACCTGGT A^CC[AT]GGT
SphI 2547 GCATGC GCATG^C
Tth111I 1727 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2976 TCTAGA T^CTAGA
XhoI 678 CTCGAG C^TCGAG
XhoII 2355 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 501 GTCGAC GT^[AC][GT]AC
3264 GTAGAC
AocI 1697 CCTAAGG CC^T[ACGT]AGG
3414 CCTGAGG
AspHI 823 GAGCTC G[AT]GC[AT]^C
2270 GTGCTC
BanII 655 GAGCCC G[GA]GC[CT]^C
823 GAGCTC
BbsI 1321 GAAGAC GAAGAC(2/6)
2779 GAAGAC
BpuAI 1321 GAAGAC GAAGAC(2/6)
2779 GAAGAC
BsaBI 353 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2066 GATGGACATC
BsaWI 261 ACCGGA [AT]^CCGG[AT]
1557 ACCGGT
BsiHKAI 823 GAGCTC G[AT]GC[AT]^C
2270 GTGCTC
BsmBI 3184 CGTCTC CGTCTC(1/5)
265 GAGACG
BstEII 2851 GGTAACC G^GT[ACGT]ACC
3397 GGTTACC
BstPI 2851 GGTAACC G^GT[ACGT]ACC
3397 GGTTACC
Bsu36I 1697 CCTAAGG CC^T[ACGT]AGG
3414 CCTGAGG
CfoI 1026 GCGC GCG^C
1158 GCGC
Eco81I 1697 CCTAAGG CC^T[ACGT]AGG
3414 CCTGAGG
EcoO65I 2851 GGTAACC G^GT[ACGT]ACC
3397 GGTTACC
HgaI 267 GACGC GACGC(5/10)
710 GACGC
HhaI 1026 GCGC GCG^C
1158 GCGC
HinP1I 1026 GCGC G^CGC
1158 GCGC
HpaI 577 GTTAAC GTT^AAC
2020 GTTAAC
MamI 353 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2066 GATGGACATC
PstI 1667 CTGCAG CTGCA^G
1923 CTGCAG
SapI 2318 GAAGAGC GCTCTTC(1/4)
1166 GCTCTTC
ScaI 474 AGTACT AGT^ACT
1063 AGTACT
SspI 65 AATATT AAT^ATT
2625 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BbvI BcgI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrI BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I