##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
Aor51HI AscI Asp718I AspEI AvrII
BamHI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BsrGI
Bst1107I BstXI BstZ17I CelII ClaI
DraIII DrdI Eam1105I Eco47III EcoRV
FbaI FseI HpaI KpnI MfeI
MluI MroI MunI NaeI NgoMIV
PacI PaeR7I PflMI PleI PmeI
PshAI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfiI SgrAI
SmaI SmlI SpeI Sse8387I SspBI
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 73 enzymes) #
###################################
AccI 1309 GTCTAC GT^[AC][GT]AC
AcyI 202 GGCGCC G[GA]^CG[CT]C
AocI 1712 CCTCAGG CC^T[ACGT]AGG
ApaI 242 GGGCCC GGGCC^C
AseI 1001 ATTAAT AT^TAAT
AsnI 1001 ATTAAT AT^TAAT
AspI 2379 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 238 CTCGGG C^[CT]CG[GA]G
AviII 3035 TGCGCA TGC^GCA
BbeI 202 GGCGCC GGCGC^C
BbrPI 2603 CACGTG CAC^GTG
BbsI 3639 GTCTTC GAAGAC(2/6)
BbvCI 2310 GCTGAGG CCTCAGC(-5/-2)
BcgI 1817 GCAAGCATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 380 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3639 GTCTTC GAAGAC(2/6)
BsaBI 1718 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 202 GGCGCC G[GA]^CG[CT]C
BsaI 2978 GGTCTC GGTCTC(1/5)
BsiEI 378 CGGCCG CG[GA][CT]^CG
BsoBI 238 CTCGGG C^[CT]CG[GA]G
BspHI 2068 TCATGA T^CATGA
BsrBI 120 GAGCGG CCGCTC(-3/-3)
BsrDI 2863 CATTGC GCAATG(2/0)
BssHII 138 GCGCGC G^CGCGC
BstBI 504 TTCGAA TT^CGAA
BstEII 3142 GGTAACC G^GT[ACGT]ACC
BstPI 3142 GGTAACC G^GT[ACGT]ACC
BstYI 1966 AGATCC [GA]^GATC[CT]
Bsu36I 1712 CCTCAGG CC^T[ACGT]AGG
CpoI 96 CGGTCCG CG^G[AT]CCG
EagI 378 CGGCCG C^GGCCG
EclXI 378 CGGCCG C^GGCCG
Eco52I 378 CGGCCG C^GGCCG
Eco81I 1712 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3142 GGTAACC G^GT[ACGT]ACC
EcoRI 2471 GAATTC G^AATTC
EcoT14I 3149 CCATGG C^C[AT][AT]GG
FspI 3035 TGCGCA TGC^GCA
HaeII 202 GGCGCC [GA]GCGC^[CT]
HgaI 1417 GACGC GACGC(5/10)
Hin1I 202 GGCGCC G[GA]^CG[CT]C
HincII 2884 GTTGAC GT[CT]^[GA]AC
HindII 2884 GTTGAC GT[CT]^[GA]AC
KasI 202 GGCGCC G^GCGCC
MamI 1718 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1966 AGATCC [GA]^GATC[CT]
NarI 202 GGCGCC GG^CGCC
NcoI 3149 CCATGG C^CATGG
NheI 4028 GCTAGC G^CTAGC
NotI 377 GCGGCCGC GC^GGCCGC
NruI 1066 TCGCGA TCG^CGA
NspV 504 TTCGAA TT^CGAA
PflFI 2379 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2603 CACGTG CAC^GTG
PmlI 2603 CACGTG CAC^GTG
PpuMI 1258 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1001 ATTAAT AT^TAAT
PstI 3795 CTGCAG CTGCA^G
RcaI 2068 TCATGA T^CATGA
RsrII 96 CGGTCCG CG^G[AT]CCG
SacI 3047 GAGCTC GAGCT^C
SfoI 202 GGCGCC GGC^GCC
SfuI 504 TTCGAA TT^CGAA
SnaBI 1637 TACGTA TAC^GTA
SphI 2191 GCATGC GCATG^C
StuI 468 AGGCCT AGG^CCT
StyI 3149 CCATGG C^C[AT][AT]GG
SwaI 3707 ATTTAAAT ATTT^AAAT
Tth111I 2379 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2720 TCTAGA T^CTAGA
XcmI 1158 CCATGCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1966 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AclI 2881 AACGTT AA^CGTT
4269 AACGTT
AfaI 4179 GTAC GT^AC
4235 GTAC
AflIII 555 ACATGT A^C[GA][CT]GT
3114 ACATGT
AgeI 689 ACCGGT A^CCGGT
1759 ACCGGT
AlwI 1967 GATCC GGATC(4/5)
3528 GGATC
AlwNI 1314 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2448 CAGACACTG
Asp700I 1037 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3155 GAAATTGTTC
BalI 1155 TGGCCA TGG^CCA
1882 TGGCCA
BanI 202 GGCGCC G^G[CT][GA]CC
841 GGTGCC
BanII 242 GGGCCC G[GA]GC[CT]^C
3047 GAGCTC
BpmI 464 CTCCAG CTGGAG(16/14)
103 CTGGAG
BsaAI 1637 TACGTA [CT]AC^GT[GA]
2603 CACGTG
BsaWI 689 ACCGGT [AT]^CCGG[AT]
1759 ACCGGT
BspLU11I 555 ACATGT A^CATGT
3114 ACATGT
BssSI 452 CACGAG CACGAG(-5/-1)
3837 CACGAG
BstAPI 2932 GCAAGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4032 GCATTGTTTGC
DpnII 1967 GATC ^GATC
3529 GATC
DraII 241 GGGGCCC [GA]G^G[ACGT]CC[CT]
1258 GGGACCT
EcoNI 1357 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2983 CCTGTCGGAGG
EcoO109I 241 GGGGCCC [GA]G^G[ACGT]CC[CT]
1258 GGGACCT
EcoT22I 2193 ATGCAT ATGCA^T
2906 ATGCAT
KspI 381 CCGCGG CCGC^GG
402 CCGCGG
MboI 1967 GATC ^GATC
3529 GATC
MluNI 1155 TGGCCA TGG^CCA
1882 TGGCCA
MscI 1155 TGGCCA TGG^CCA
1882 TGGCCA
NdeI 932 CATATG CA^TATG
3922 CATATG
NdeII 1967 GATC ^GATC
3529 GATC
NsiI 2193 ATGCAT ATGCA^T
2906 ATGCAT
PinAI 689 ACCGGT A^CCGGT
1759 ACCGGT
Psp1406I 2881 AACGTT AA^CGTT
4269 AACGTT
RsaI 4179 GTAC GT^AC
4235 GTAC
SacII 381 CCGCGG CCGC^GG
402 CCGCGG
Sau3AI 1967 GATC ^GATC
3529 GATC
XmnI 1037 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3155 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcsI AluI Alw44I
ApaLI ApoI AspHI AvaII BbvI
BcnI BfaI BmyI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraI DsaI EaeI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV NspI Sau96I
ScrFI SfaNI SfcI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I