##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccII AclI AgeI Alw44I ApaLI
AscI AseI AsnI AspI AviII
BbeI BclI BglII BsaBI BsiEI
BsiWI BspDI BspHI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoRV EcoT22I FbaI FseI FspI
HpaI KasI KspI MamI MfeI
MluI MunI MvnI NarI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 458 GACGTC GACGT^C
Acc65I 3934 GGTACC G^GTACC
AccI 3320 GTAGAC GT^[AC][GT]AC
AccIII 929 TCCGGA T^CCGGA
AflII 3291 CTTAAG C^TTAAG
AhdI 3691 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 535 AGCGCT AGC^GCT
Asp718I 3934 GGTACC G^GTACC
AspEI 3691 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3839 CCTAGG C^CTAGG
BbsI 10 GTCTTC GAAGAC(2/6)
BciVI 1582 GTATCC GTATCC(6/5)
BfrI 3291 CTTAAG C^TTAAG
BlnI 3839 CCTAGG C^CTAGG
BpuAI 10 GTCTTC GAAGAC(2/6)
BsaWI 929 TCCGGA [AT]^CCGG[AT]
BseAI 929 TCCGGA T^CCGGA
BsmBI 506 GAGACG CGTCTC(1/5)
BsmI 2649 GCATTC GAATGC(1/-1)
Bsp1407I 2180 TGTACA T^GTACA
BspEI 929 TCCGGA T^CCGGA
BspLU11I 2669 ACATGT A^CATGT
BsrGI 2180 TGTACA T^GTACA
BstEII 341 GGTGACC G^GT[ACGT]ACC
BstPI 341 GGTGACC G^GT[ACGT]ACC
DraI 4108 TTTAAA TTT^AAA
DraIII 723 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3691 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 535 AGCGCT AGC^GCT
EcoO65I 341 GGTGACC G^GT[ACGT]ACC
EcoRI 2089 GAATTC G^AATTC
HincII 1951 GTTGAC GT[CT]^[GA]AC
HindII 1951 GTTGAC GT[CT]^[GA]AC
HindIII 4883 AAGCTT A^AGCTT
KpnI 3934 GGTACC GGTAC^C
MroI 929 TCCGGA T^CCGGA
NheI 2223 GCTAGC G^CTAGC
ScaI 3425 AGTACT AGT^ACT
SfiI 2949 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3436 CCCGGG CCC^GGG
SspBI 2180 TGTACA T^GTACA
TaqI 627 TCGA T^CGA
TthHB8I 627 TCGA T^CGA
XmaCI 3436 CCCGGG C^CCGGG
XmaI 3436 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 2669 ACATGT A^C[GA][CT]GT
4636 ACGTGT
Asp700I 4059 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4135 GAAAGTTTTC
AvaI 3436 CCCGGG C^[CT]CG[GA]G
3788 CCCGAG
BamHI 1247 GGATCC G^GATCC
4298 GGATCC
BbrPI 3877 CACGTG CAC^GTG
4635 CACGTG
BcgI 3913 CGACAGAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2162 GCAGAAGTCTCG
BsaAI 3877 CACGTG [CT]AC^GT[GA]
4635 CACGTG
BsoBI 3436 CCCGGG C^[CT]CG[GA]G
3788 CCCGAG
BsrBI 875 CCGCTC CCGCTC(-3/-3)
2894 CCGCTC
BsrDI 1087 CATTGC GCAATG(2/0)
2854 GCAATG
BstAPI 324 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2572 GCATCTTCTGC
CfoI 262 GCGC GCG^C
536 GCGC
HaeII 261 AGCGCC [GA]GCGC^[CT]
535 AGCGCT
HhaI 262 GCGC GCG^C
536 GCGC
HinP1I 262 GCGC G^CGC
536 GCGC
NaeI 739 GCCGGC GCC^GGC
3308 GCCGGC
NgoMIV 739 GCCGGC G^CCGGC
3308 GCCGGC
PmaCI 3877 CACGTG CAC^GTG
4635 CACGTG
PmlI 3877 CACGTG CAC^GTG
4635 CACGTG
XmnI 4059 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4135 GAAAGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XbaI
XcmI XhoII