##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AhdI AscI
AseI AsnI Asp718I AspEI AvrII
BclI BlnI BsaBI BsiWI BspDI
BspLU11I BssSI BstBI BstEII BstPI
ClaI DraIII EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MunI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflMI PmeI PshAI
PshBI Psp1406I PvuI SalI SexAI
SfuI SgrAI SnaBI SpeI SphI
SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 34 CTTAAG C^TTAAG
Alw44I 3419 GTGCAC G^TGCAC
Aor51HI 2079 AGCGCT AGC^GCT
ApaLI 3419 GTGCAC G^TGCAC
Asp700I 283 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1512 TGCGCA TGC^GCA
BamHI 3159 GGATCC G^GATCC
BbeI 814 GGCGCC GGCGC^C
BcgI 307 GCACAGATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1413 GTATCC GTATCC(6/5)
BfrI 34 CTTAAG C^TTAAG
BlpI 182 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 182 GCTGAGC GC^T[ACGT]AGC
BsiEI 385 CGGTCG CG[GA][CT]^CG
BsmBI 3215 GAGACG CGTCTC(1/5)
BspHI 674 TCATGA T^CATGA
BsrDI 2484 GCAATG GCAATG(2/0)
BssHII 225 GCGCGC G^CGCGC
Bst1107I 3007 GTATAC GTA^TAC
BstAPI 1666 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3007 GTATAC GTA^TAC
CelII 182 GCTGAGC GC^T[ACGT]AGC
CpoI 84 CGGACCG CG^G[AT]CCG
DraI 4063 TTTAAA TTT^AAA
DrdI 86 GACCGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2079 AGCGCT AGC^GCT
EcoNI 2107 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1901 GAATTC G^AATTC
FspI 1512 TGCGCA TGC^GCA
HincII 4120 GTCAAC GT[CT]^[GA]AC
HindII 4120 GTCAAC GT[CT]^[GA]AC
KasI 814 GGCGCC G^GCGCC
KspI 550 CCGCGG CCGC^GG
NarI 814 GGCGCC GG^CGCC
NheI 2341 GCTAGC G^CTAGC
RcaI 674 TCATGA T^CATGA
RsrII 84 CGGACCG CG^G[AT]CCG
SacII 550 CCGCGG CCGC^GG
ScaI 1965 AGTACT AGT^ACT
SfiI 991 GGCCACGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 814 GGCGCC GGC^GCC
SspI 4093 AATATT AAT^ATT
XcmI 3141 CCAACAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 283 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 3007 GTATAC GT^[AC][GT]AC
3792 GTCTAC
AccIII 198 TCCGGA T^CCGGA
3975 TCCGGA
AflIII 3415 ACACGT A^C[GA][CT]GT
3423 ACACGT
AgeI 1935 ACCGGT A^CCGGT
2635 ACCGGT
ApaI 1429 GGGCCC GGGCC^C
2245 GGGCCC
AspI 1607 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2352 GACTGTGTC
AvaI 968 CCCGGG C^[CT]CG[GA]G
2567 CCCGGG
BanI 489 GGTGCC G^G[CT][GA]CC
814 GGCGCC
BbrPI 3416 CACGTG CAC^GTG
3424 CACGTG
BbvCI 1029 CCTCAGC CCTCAGC(-5/-2)
622 GCTGAGG
BglII 367 AGATCT A^GATCT
2604 AGATCT
BseAI 198 TCCGGA T^CCGGA
3975 TCCGGA
BsmI 1799 GAATGC GAATGC(1/-1)
3512 GAATGC
BsoBI 968 CCCGGG C^[CT]CG[GA]G
2567 CCCGGG
Bsp1407I 299 TGTACA T^GTACA
3739 TGTACA
BspEI 198 TCCGGA T^CCGGA
3975 TCCGGA
BsrFI 1935 ACCGGT [GA]^CCGG[CT]
2635 ACCGGT
BsrGI 299 TGTACA T^GTACA
3739 TGTACA
BstXI 1362 CCATTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2901 CCAAAAATTTGG
Cfr10I 1935 ACCGGT [GA]^CCGG[CT]
2635 ACCGGT
MroI 198 TCCGGA T^CCGGA
3975 TCCGGA
NcoI 938 CCATGG C^CATGG
1974 CCATGG
PflFI 1607 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2352 GACTGTGTC
PinAI 1935 ACCGGT A^CCGGT
2635 ACCGGT
PmaCI 3416 CACGTG CAC^GTG
3424 CACGTG
PmlI 3416 CACGTG CAC^GTG
3424 CACGTG
SacI 185 GAGCTC GAGCT^C
3364 GAGCTC
SapI 2548 GAAGAGC GCTCTTC(1/4)
3525 GAAGAGC
SbfI 1663 CCTGCAGG CCTGCA^GG
2410 CCTGCAGG
SmaI 968 CCCGGG CCC^GGG
2567 CCCGGG
Sse8387I 1663 CCTGCAGG CCTGCA^GG
2410 CCTGCAGG
SspBI 299 TGTACA T^GTACA
3739 TGTACA
StuI 3764 AGGCCT AGG^CCT
3783 AGGCCT
Tth111I 1607 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2352 GACTGTGTC
XmaCI 968 CCCGGG C^CCGGG
2567 CCCGGG
XmaI 968 CCCGGG C^CCGGG
2567 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BalI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII