##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AccI        AccII       AccIII      AclI        AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AspEI       AviII       BglI        BseAI       BsiEI       
BsiWI       BsmBI       BspEI       BsrBI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstZ17I     
CpoI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      FseI        FspI        HgaI        KspI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
SspI        SwaI        XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII        2367  GACGTC               GACGT^C
Acc65I       4118  GGTACC               G^GTACC
AflII        3698  CTTAAG               C^TTAAG
AocI         2540  CCTCAGG              CC^T[ACGT]AGG
ApaI         1327  GGGCCC               GGGCC^C
AseI         2613  ATTAAT               AT^TAAT
AsnI         2613  ATTAAT               AT^TAAT
Asp718I      4118  GGTACC               G^GTACC
AvaI         1348  CCCGAG               C^[CT]CG[GA]G
BamHI        4168  GGATCC               G^GATCC
BbeI          695  GGCGCC               GGCGC^C
BbrPI         136  CACGTG               CAC^GTG
BcgI         3801  GCAACAGATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         566  GTATCC               GTATCC(6/5)
BclI          820  TGATCA               T^GATCA
BfrI         3698  CTTAAG               C^TTAAG
BglII           4  AGATCT               A^GATCT
BsaAI         136  CACGTG               [CT]AC^GT[GA]
BsaBI        1268  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          153  GAGACC               GGTCTC(1/5)
BsaWI        4115  TCCGGT               [AT]^CCGG[AT]
BsoBI        1348  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     3885  TGTACA               T^GTACA
BspDI         852  ATCGAT               AT^CGAT
BsrFI        1224  ACCGGC               [GA]^CCGG[CT]
BsrGI        3885  TGTACA               T^GTACA
BstAPI        740  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       2540  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1224  ACCGGC               [GA]^CCGG[CT]
ClaI          852  ATCGAT               AT^CGAT
DraIII       2603  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       2540  CCTCAGG              CC^T[ACGT]AGG
EcoRI         933  GAATTC               G^AATTC
EcoRV         798  GATATC               GAT^ATC
EcoT22I      3546  ATGCAT               ATGCA^T
FauI         4076  CCCGC                CCCGC(4/6)
FbaI          820  TGATCA               T^GATCA
HindIII      3124  AAGCTT               A^AGCTT
KasI          695  GGCGCC               G^GCGCC
KpnI         4118  GGTACC               GGTAC^C
MamI         1268  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI          695  GGCGCC               GG^CGCC
NcoI         2320  CCATGG               C^CATGG
NsiI         3546  ATGCAT               ATGCA^T
PmaCI         136  CACGTG               CAC^GTG
PmlI          136  CACGTG               CAC^GTG
PshBI        2613  ATTAAT               AT^TAAT
SbfI          746  CCTGCAGG             CCTGCA^GG
ScaI         2738  AGTACT               AGT^ACT
SfoI          695  GGCGCC               GGC^GCC
SpeI         1896  ACTAGT               A^CTAGT
Sse8387I      746  CCTGCAGG             CCTGCA^GG
SspBI        3885  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcyI          695  GGCGCC               G[GA]^CG[CT]C
             2367  GACGTC              
AflIII       1546  ACATGT               A^C[GA][CT]GT
             2850  ACATGT              
Asp700I      2678  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3097  GAACCAGTTC          
AspI          954  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3058  GACCAGGTC           
BalI          916  TGGCCA               TGG^CCA
             1575  TGGCCA              
BbvCI         663  CCTCAGC              CCTCAGC(-5/-2)
             4009  CCTCAGC             
BlpI         3121  GCTAAGC              GC^T[ACGT]AGC
             3918  GCTTAGC             
Bpu1102I     3121  GCTAAGC              GC^T[ACGT]AGC
             3918  GCTTAGC             
BsaHI         695  GGCGCC               G[GA]^CG[CT]C
             2367  GACGTC              
BsmI         1421  GCATTC               GAATGC(1/-1)
             2005  GCATTC              
BspHI         399  TCATGA               T^CATGA
              941  TCATGA              
BspLU11I     1546  ACATGT               A^CATGT
             2850  ACATGT              
BstEII        574  GGTCACC              G^GT[ACGT]ACC
             4095  GGTCACC             
BstPI         574  GGTCACC              G^GT[ACGT]ACC
             4095  GGTCACC             
BstXI        1205  CCAGAATCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1234  CCATTATGCTGG        
BstYI           4  AGATCT               [GA]^GATC[CT]
             4168  GGATCC              
CelII        3121  GCTAAGC              GC^T[ACGT]AGC
             3918  GCTTAGC             
DrdI          708  GACGAGAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2333  GACCTTGAGGTC        
DsaI          570  CCGTGG               C^C[GA][CT]GG
             2320  CCATGG              
EcoNI        1875  CCTGAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4009  CCTCAGCCAGG         
EcoO65I       574  GGTCACC              G^GT[ACGT]ACC
             4095  GGTCACC             
Hin1I         695  GGCGCC               G[GA]^CG[CT]C
             2367  GACGTC              
MflI            4  AGATCT               [GA]^GATC[CT]
             4168  GGATCC              
MluNI         916  TGGCCA               TGG^CCA
             1575  TGGCCA              
MscI          916  TGGCCA               TGG^CCA
             1575  TGGCCA              
MspA1I         50  CAGCTG               C[AC]G^C[GT]G
              239  CAGCTG              
PflFI         954  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3058  GACCAGGTC           
PstI          747  CTGCAG               CTGCA^G
             2362  CTGCAG              
PvuII          50  CAGCTG               CAG^CTG
              239  CAGCTG              
RcaI          399  TCATGA               T^CATGA
              941  TCATGA              
SexAI         519  ACCAGGT              A^CC[AT]GGT
             3059  ACCAGGT             
SfcI          747  CTGCAG               C^T[GA][CT]AG
             2362  CTGCAG              
StuI          252  AGGCCT               AGG^CCT
             4017  AGGCCT              
Tth111I       954  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3058  GACCAGGTC           
XhoII           4  AGATCT               [GA]^GATC[CT]
             4168  GGATCC              
XmnI         2678  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3097  GAACCAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       AvrII       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BlnI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaI        HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I