##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccI AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AviII BglI BseAI BsiEI
BsiWI BsmBI BspEI BsrBI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I FseI FspI HgaI KspI
MfeI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SfiI
SfuI SgrAI SmaI SnaBI SphI
SspI SwaI XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 2367 GACGTC GACGT^C
Acc65I 4118 GGTACC G^GTACC
AflII 3698 CTTAAG C^TTAAG
AocI 2540 CCTCAGG CC^T[ACGT]AGG
ApaI 1327 GGGCCC GGGCC^C
AseI 2613 ATTAAT AT^TAAT
AsnI 2613 ATTAAT AT^TAAT
Asp718I 4118 GGTACC G^GTACC
AvaI 1348 CCCGAG C^[CT]CG[GA]G
BamHI 4168 GGATCC G^GATCC
BbeI 695 GGCGCC GGCGC^C
BbrPI 136 CACGTG CAC^GTG
BcgI 3801 GCAACAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 566 GTATCC GTATCC(6/5)
BclI 820 TGATCA T^GATCA
BfrI 3698 CTTAAG C^TTAAG
BglII 4 AGATCT A^GATCT
BsaAI 136 CACGTG [CT]AC^GT[GA]
BsaBI 1268 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 153 GAGACC GGTCTC(1/5)
BsaWI 4115 TCCGGT [AT]^CCGG[AT]
BsoBI 1348 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3885 TGTACA T^GTACA
BspDI 852 ATCGAT AT^CGAT
BsrFI 1224 ACCGGC [GA]^CCGG[CT]
BsrGI 3885 TGTACA T^GTACA
BstAPI 740 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2540 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1224 ACCGGC [GA]^CCGG[CT]
ClaI 852 ATCGAT AT^CGAT
DraIII 2603 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2540 CCTCAGG CC^T[ACGT]AGG
EcoRI 933 GAATTC G^AATTC
EcoRV 798 GATATC GAT^ATC
EcoT22I 3546 ATGCAT ATGCA^T
FauI 4076 CCCGC CCCGC(4/6)
FbaI 820 TGATCA T^GATCA
HindIII 3124 AAGCTT A^AGCTT
KasI 695 GGCGCC G^GCGCC
KpnI 4118 GGTACC GGTAC^C
MamI 1268 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 695 GGCGCC GG^CGCC
NcoI 2320 CCATGG C^CATGG
NsiI 3546 ATGCAT ATGCA^T
PmaCI 136 CACGTG CAC^GTG
PmlI 136 CACGTG CAC^GTG
PshBI 2613 ATTAAT AT^TAAT
SbfI 746 CCTGCAGG CCTGCA^GG
ScaI 2738 AGTACT AGT^ACT
SfoI 695 GGCGCC GGC^GCC
SpeI 1896 ACTAGT A^CTAGT
Sse8387I 746 CCTGCAGG CCTGCA^GG
SspBI 3885 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 695 GGCGCC G[GA]^CG[CT]C
2367 GACGTC
AflIII 1546 ACATGT A^C[GA][CT]GT
2850 ACATGT
Asp700I 2678 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3097 GAACCAGTTC
AspI 954 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3058 GACCAGGTC
BalI 916 TGGCCA TGG^CCA
1575 TGGCCA
BbvCI 663 CCTCAGC CCTCAGC(-5/-2)
4009 CCTCAGC
BlpI 3121 GCTAAGC GC^T[ACGT]AGC
3918 GCTTAGC
Bpu1102I 3121 GCTAAGC GC^T[ACGT]AGC
3918 GCTTAGC
BsaHI 695 GGCGCC G[GA]^CG[CT]C
2367 GACGTC
BsmI 1421 GCATTC GAATGC(1/-1)
2005 GCATTC
BspHI 399 TCATGA T^CATGA
941 TCATGA
BspLU11I 1546 ACATGT A^CATGT
2850 ACATGT
BstEII 574 GGTCACC G^GT[ACGT]ACC
4095 GGTCACC
BstPI 574 GGTCACC G^GT[ACGT]ACC
4095 GGTCACC
BstXI 1205 CCAGAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1234 CCATTATGCTGG
BstYI 4 AGATCT [GA]^GATC[CT]
4168 GGATCC
CelII 3121 GCTAAGC GC^T[ACGT]AGC
3918 GCTTAGC
DrdI 708 GACGAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2333 GACCTTGAGGTC
DsaI 570 CCGTGG C^C[GA][CT]GG
2320 CCATGG
EcoNI 1875 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4009 CCTCAGCCAGG
EcoO65I 574 GGTCACC G^GT[ACGT]ACC
4095 GGTCACC
Hin1I 695 GGCGCC G[GA]^CG[CT]C
2367 GACGTC
MflI 4 AGATCT [GA]^GATC[CT]
4168 GGATCC
MluNI 916 TGGCCA TGG^CCA
1575 TGGCCA
MscI 916 TGGCCA TGG^CCA
1575 TGGCCA
MspA1I 50 CAGCTG C[AC]G^C[GT]G
239 CAGCTG
PflFI 954 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3058 GACCAGGTC
PstI 747 CTGCAG CTGCA^G
2362 CTGCAG
PvuII 50 CAGCTG CAG^CTG
239 CAGCTG
RcaI 399 TCATGA T^CATGA
941 TCATGA
SexAI 519 ACCAGGT A^CC[AT]GGT
3059 ACCAGGT
SfcI 747 CTGCAG C^T[GA][CT]AG
2362 CTGCAG
StuI 252 AGGCCT AGG^CCT
4017 AGGCCT
Tth111I 954 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3058 GACCAGGTC
XhoII 4 AGATCT [GA]^GATC[CT]
4168 GGATCC
XmnI 2678 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3097 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII AvrII
BanI BanII BbsI BbvI BcnI
BfaI BlnI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I