################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AccI AccII AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AspEI AviII BglI BseAI BsiEI BsiWI BsmBI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CpoI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI HgaI KspI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SfiI SfuI SgrAI SmaI SnaBI SphI SspI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 2367 GACGTC GACGT^C Acc65I 4118 GGTACC G^GTACC AflII 3698 CTTAAG C^TTAAG AocI 2540 CCTCAGG CC^T[ACGT]AGG ApaI 1327 GGGCCC GGGCC^C AseI 2613 ATTAAT AT^TAAT AsnI 2613 ATTAAT AT^TAAT Asp718I 4118 GGTACC G^GTACC AvaI 1348 CCCGAG C^[CT]CG[GA]G BamHI 4168 GGATCC G^GATCC BbeI 695 GGCGCC GGCGC^C BbrPI 136 CACGTG CAC^GTG BcgI 3801 GCAACAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 566 GTATCC GTATCC(6/5) BclI 820 TGATCA T^GATCA BfrI 3698 CTTAAG C^TTAAG BglII 4 AGATCT A^GATCT BsaAI 136 CACGTG [CT]AC^GT[GA] BsaBI 1268 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 153 GAGACC GGTCTC(1/5) BsaWI 4115 TCCGGT [AT]^CCGG[AT] BsoBI 1348 CCCGAG C^[CT]CG[GA]G Bsp1407I 3885 TGTACA T^GTACA BspDI 852 ATCGAT AT^CGAT BsrFI 1224 ACCGGC [GA]^CCGG[CT] BsrGI 3885 TGTACA T^GTACA BstAPI 740 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2540 CCTCAGG CC^T[ACGT]AGG Cfr10I 1224 ACCGGC [GA]^CCGG[CT] ClaI 852 ATCGAT AT^CGAT DraIII 2603 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2540 CCTCAGG CC^T[ACGT]AGG EcoRI 933 GAATTC G^AATTC EcoRV 798 GATATC GAT^ATC EcoT22I 3546 ATGCAT ATGCA^T FauI 4076 CCCGC CCCGC(4/6) FbaI 820 TGATCA T^GATCA HindIII 3124 AAGCTT A^AGCTT KasI 695 GGCGCC G^GCGCC KpnI 4118 GGTACC GGTAC^C MamI 1268 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 695 GGCGCC GG^CGCC NcoI 2320 CCATGG C^CATGG NsiI 3546 ATGCAT ATGCA^T PmaCI 136 CACGTG CAC^GTG PmlI 136 CACGTG CAC^GTG PshBI 2613 ATTAAT AT^TAAT SbfI 746 CCTGCAGG CCTGCA^GG ScaI 2738 AGTACT AGT^ACT SfoI 695 GGCGCC GGC^GCC SpeI 1896 ACTAGT A^CTAGT Sse8387I 746 CCTGCAGG CCTGCA^GG SspBI 3885 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AcyI 695 GGCGCC G[GA]^CG[CT]C 2367 GACGTC AflIII 1546 ACATGT A^C[GA][CT]GT 2850 ACATGT Asp700I 2678 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3097 GAACCAGTTC AspI 954 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3058 GACCAGGTC BalI 916 TGGCCA TGG^CCA 1575 TGGCCA BbvCI 663 CCTCAGC CCTCAGC(-5/-2) 4009 CCTCAGC BlpI 3121 GCTAAGC GC^T[ACGT]AGC 3918 GCTTAGC Bpu1102I 3121 GCTAAGC GC^T[ACGT]AGC 3918 GCTTAGC BsaHI 695 GGCGCC G[GA]^CG[CT]C 2367 GACGTC BsmI 1421 GCATTC GAATGC(1/-1) 2005 GCATTC BspHI 399 TCATGA T^CATGA 941 TCATGA BspLU11I 1546 ACATGT A^CATGT 2850 ACATGT BstEII 574 GGTCACC G^GT[ACGT]ACC 4095 GGTCACC BstPI 574 GGTCACC G^GT[ACGT]ACC 4095 GGTCACC BstXI 1205 CCAGAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1234 CCATTATGCTGG BstYI 4 AGATCT [GA]^GATC[CT] 4168 GGATCC CelII 3121 GCTAAGC GC^T[ACGT]AGC 3918 GCTTAGC DrdI 708 GACGAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2333 GACCTTGAGGTC DsaI 570 CCGTGG C^C[GA][CT]GG 2320 CCATGG EcoNI 1875 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4009 CCTCAGCCAGG EcoO65I 574 GGTCACC G^GT[ACGT]ACC 4095 GGTCACC Hin1I 695 GGCGCC G[GA]^CG[CT]C 2367 GACGTC MflI 4 AGATCT [GA]^GATC[CT] 4168 GGATCC MluNI 916 TGGCCA TGG^CCA 1575 TGGCCA MscI 916 TGGCCA TGG^CCA 1575 TGGCCA MspA1I 50 CAGCTG C[AC]G^C[GT]G 239 CAGCTG PflFI 954 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3058 GACCAGGTC PstI 747 CTGCAG CTGCA^G 2362 CTGCAG PvuII 50 CAGCTG CAG^CTG 239 CAGCTG RcaI 399 TCATGA T^CATGA 941 TCATGA SexAI 519 ACCAGGT A^CC[AT]GGT 3059 ACCAGGT SfcI 747 CTGCAG C^T[GA][CT]AG 2362 CTGCAG StuI 252 AGGCCT AGG^CCT 4017 AGGCCT Tth111I 954 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3058 GACCAGGTC XhoII 4 AGATCT [GA]^GATC[CT] 4168 GGATCC XmnI 2678 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3097 GAACCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII AvrII BanI BanII BbsI BbvI BcnI BfaI BlnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I