##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
ApaI AscI Asp718I AvaI BbeI
BcgI BclI BfrI BsaBI BseAI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI ClaI CpoI
EagI EclXI Eco52I EcoO65I FbaI
FseI HincII HindII HindIII HpaI
KasI KpnI MamI MluI MroI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PmeI
PshAI Psp1406I PvuI RsrII SacI
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SwaI
TaqI TthHB8I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2150 GACGTC GACGT^C
AhdI 4053 GACTGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1204 AGCGCT AGC^GCT
Asp700I 1759 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4053 GACTGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1710 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1193 TGCGCA TGC^GCA
BamHI 1026 GGATCC G^GATCC
BanI 3146 GGCACC G^G[CT][GA]CC
BbrPI 2024 CACGTG CAC^GTG
BbsI 3931 GTCTTC GAAGAC(2/6)
BbvCI 1576 GCTGAGG CCTCAGC(-5/-2)
BciVI 275 GGATAC GTATCC(6/5)
BpuAI 3931 GTCTTC GAAGAC(2/6)
BsiEI 745 CGGTCG CG[GA][CT]^CG
BsmI 921 GCATTC GAATGC(1/-1)
BspHI 2198 TCATGA T^CATGA
BsrBI 711 CCGCTC CCGCTC(-3/-3)
Bst1107I 3297 GTATAC GTA^TAC
BstZ17I 3297 GTATAC GTA^TAC
DraIII 2803 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2129 GACCACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4053 GACTGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1204 AGCGCT AGC^GCT
EcoT22I 1317 ATGCAT ATGCA^T
FspI 1193 TGCGCA TGC^GCA
KspI 1014 CCGCGG CCGC^GG
MfeI 2716 CAATTG C^AATTG
MslI 22 CATTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2716 CAATTG C^AATTG
NheI 3984 GCTAGC G^CTAGC
NsiI 1317 ATGCAT ATGCA^T
NspI 2347 GCATGC [GA]CATG^[CT]
PflFI 1710 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2024 CACGTG CAC^GTG
PmlI 2024 CACGTG CAC^GTG
RcaI 2198 TCATGA T^CATGA
SacII 1014 CCGCGG CCGC^GG
SbfI 2326 CCTGCAGG CCTGCA^GG
SphI 2347 GCATGC GCATG^C
Sse8387I 2326 CCTGCAGG CCTGCA^GG
Tth111I 1710 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1759 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 1015 CGCG CG^CG
3621 CGCG
AcyI 874 GACGCC G[GA]^CG[CT]C
2150 GACGTC
AgeI 1043 ACCGGT A^CCGGT
1218 ACCGGT
Alw44I 767 GTGCAC G^TGCAC
3788 GTGCAC
AocI 637 CCTCAGG CC^T[ACGT]AGG
3082 CCTTAGG
ApaLI 767 GTGCAC G^TGCAC
3788 GTGCAC
AseI 357 ATTAAT AT^TAAT
543 ATTAAT
AsnI 357 ATTAAT AT^TAAT
543 ATTAAT
AvrII 1567 CCTAGG C^CTAGG
4298 CCTAGG
BglI 2897 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3487 GCCTGTTAGGC
BlnI 1567 CCTAGG C^CTAGG
4298 CCTAGG
BlpI 3426 GCTTAGC GC^T[ACGT]AGC
3742 GCTCAGC
Bpu1102I 3426 GCTTAGC GC^T[ACGT]AGC
3742 GCTCAGC
BsaAI 1244 CACGTA [CT]AC^GT[GA]
2024 CACGTG
BsaHI 874 GACGCC G[GA]^CG[CT]C
2150 GACGTC
BsaWI 1043 ACCGGT [AT]^CCGG[AT]
1218 ACCGGT
BspMI 617 ACCTGC ACCTGC(4/8)
2329 GCAGGT
BsrDI 1120 GCAATG GCAATG(2/0)
1880 GCAATG
BsrFI 1043 ACCGGT [GA]^CCGG[CT]
1218 ACCGGT
BstUI 1015 CGCG CG^CG
3621 CGCG
Bsu36I 637 CCTCAGG CC^T[ACGT]AGG
3082 CCTTAGG
CelII 3426 GCTTAGC GC^T[ACGT]AGC
3742 GCTCAGC
Cfr10I 1043 ACCGGT [GA]^CCGG[CT]
1218 ACCGGT
Eco81I 637 CCTCAGG CC^T[ACGT]AGG
3082 CCTTAGG
EcoNI 3810 CCTGCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4006 CCTGTCCCAGG
EcoRV 247 GATATC GAT^ATC
2091 GATATC
Hin1I 874 GACGCC G[GA]^CG[CT]C
2150 GACGTC
MvnI 1015 CGCG CG^CG
3621 CGCG
PflMI 57 CCAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
266 CCATGGAATGG
PinAI 1043 ACCGGT A^CCGGT
1218 ACCGGT
PshBI 357 ATTAAT AT^TAAT
543 ATTAAT
PstI 1051 CTGCAG CTGCA^G
2327 CTGCAG
PvuII 2314 CAGCTG CAG^CTG
3643 CAGCTG
ScaI 101 AGTACT AGT^ACT
3219 AGTACT
SpeI 2190 ACTAGT A^CTAGT
3012 ACTAGT
StuI 1506 AGGCCT AGG^CCT
3079 AGGCCT
Van91I 57 CCAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
266 CCATGGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII