##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
AocI Aor51HI ApaLI AscI Asp718I
AvaI AvrII BamHI BbeI BbvCI
BcgI BciVI BclI BcnI BfrI
BlnI BsaBI BsaI BsiEI BsiWI
BsmBI BsoBI BspDI BsrBI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FbaI FseI HaeII
HindIII KasI KpnI KspI MamI
MfeI MluI MunI NarI NciI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 3508 GACGTC GACGT^C
AccI 710 GTAGAC GT^[AC][GT]AC
AccIII 2740 TCCGGA T^CCGGA
AhdI 4200 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3437 ATTAAT AT^TAAT
AsnI 3437 ATTAAT AT^TAAT
AspEI 4200 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1853 TGCGCA TGC^GCA
BalI 1798 TGGCCA TGG^CCA
BbrPI 1897 CACGTG CAC^GTG
BglI 1804 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1379 AGATCT A^GATCT
BsaAI 1897 CACGTG [CT]AC^GT[GA]
BseAI 2740 TCCGGA T^CCGGA
BspEI 2740 TCCGGA T^CCGGA
BspHI 2395 TCATGA T^CATGA
BspLU11I 3153 ACATGT A^CATGT
BsrFI 3280 GCCGGC [GA]^CCGG[CT]
Cfr10I 3280 GCCGGC [GA]^CCGG[CT]
DraIII 4096 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1445 CCATGG C^C[GA][CT]GG
EaeI 1798 TGGCCA [CT]^GGCC[GA]
Eam1105I 4200 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2375 GAATTC G^AATTC
EcoT14I 1445 CCATGG C^C[AT][AT]GG
EcoT22I 3147 ATGCAT ATGCA^T
FspI 1853 TGCGCA TGC^GCA
HgaI 3043 GACGC GACGC(5/10)
HpaI 3457 GTTAAC GTT^AAC
MluNI 1798 TGGCCA TGG^CCA
MroI 2740 TCCGGA T^CCGGA
MscI 1798 TGGCCA TGG^CCA
NaeI 3280 GCCGGC GCC^GGC
NcoI 1445 CCATGG C^CATGG
NgoMIV 3280 GCCGGC G^CCGGC
NheI 1825 GCTAGC G^CTAGC
NsiI 3147 ATGCAT ATGCA^T
PmaCI 1897 CACGTG CAC^GTG
PmlI 1897 CACGTG CAC^GTG
PshBI 3437 ATTAAT AT^TAAT
RcaI 2395 TCATGA T^CATGA
SacI 58 GAGCTC GAGCT^C
SapI 1933 GAAGAGC GCTCTTC(1/4)
ScaI 3066 AGTACT AGT^ACT
SexAI 3196 ACCAGGT A^CC[AT]GGT
SmlI 3608 CTTGAG C^T[CT][GA]AG
SphI 571 GCATGC GCATG^C
StyI 1445 CCATGG C^C[AT][AT]GG
TaqI 3004 TCGA T^CGA
TthHB8I 3004 TCGA T^CGA
XcmI 4098 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 3043 GACGCC G[GA]^CG[CT]C
3508 GACGTC
AflIII 1898 ACGTGT A^C[GA][CT]GT
3153 ACATGT
ApaI 8 GGGCCC GGGCC^C
37 GGGCCC
Asp700I 1564 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3019 GAATGGCTTC
AspHI 58 GAGCTC G[AT]GC[AT]^C
4146 GTGCTC
AspI 805 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3824 GACTCTGTC
BanI 2566 GGCACC G^G[CT][GA]CC
3270 GGCACC
BlpI 664 GCTCAGC GC^T[ACGT]AGC
4158 GCTGAGC
Bpu1102I 664 GCTCAGC GC^T[ACGT]AGC
4158 GCTGAGC
BsaHI 3043 GACGCC G[GA]^CG[CT]C
3508 GACGTC
BsaWI 1945 ACCGGA [AT]^CCGG[AT]
2740 TCCGGA
BseRI 1521 CTCCTC GAGGAG(10/8)
656 GAGGAG
BsiHKAI 58 GAGCTC G[AT]GC[AT]^C
4146 GTGCTC
Bsp1407I 1862 TGTACA T^GTACA
3186 TGTACA
BsrGI 1862 TGTACA T^GTACA
3186 TGTACA
BssHII 43 GCGCGC G^CGCGC
3128 GCGCGC
CelII 664 GCTCAGC GC^T[ACGT]AGC
4158 GCTGAGC
EcoNI 1906 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4128 CCTTTGGAAGG
Hin1I 3043 GACGCC G[GA]^CG[CT]C
3508 GACGTC
HincII 1415 GTTGAC GT[CT]^[GA]AC
3457 GTTAAC
HindII 1415 GTTGAC GT[CT]^[GA]AC
3457 GTTAAC
MspA1I 737 CAGCTG C[AC]G^C[GT]G
2751 CAGCTG
PflFI 805 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3824 GACTCTGTC
PflMI 3251 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4095 CCACCATGTGG
PvuII 737 CAGCTG CAG^CTG
2751 CAGCTG
SpeI 174 ACTAGT A^CTAGT
290 ACTAGT
SspBI 1862 TGTACA T^GTACA
3186 TGTACA
SspI 1084 AATATT AAT^ATT
3548 AATATT
Tth111I 805 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3824 GACTCTGTC
Van91I 3251 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4095 CCACCATGTGG
XmnI 1564 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3019 GAATGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII