##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AviII BbeI BclI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmI BspDI BspEI BspHI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV FbaI FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
KspI MamI MluI MroI MvnI
NarI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI SspI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 1388 AAATTT [GA]^AATT[CT]
AflII 1620 CTTAAG C^TTAAG
AocI 325 CCTTAGG CC^T[ACGT]AGG
ApoI 1388 AAATTT [GA]^AATT[CT]
Asp700I 1336 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1648 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1805 GGATCC G^GATCC
BbrPI 3028 CACGTG CAC^GTG
BcgI 3294 GCAGTGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1216 GGATAC GTATCC(6/5)
BfrI 1620 CTTAAG C^TTAAG
BsaWI 2733 ACCGGA [AT]^CCGG[AT]
BsmBI 2453 GAGACG CGTCTC(1/5)
BspLU11I 2280 ACATGT A^CATGT
BsrBI 3538 GAGCGG CCGCTC(-3/-3)
BsrDI 2108 CATTGC GCAATG(2/0)
BsrFI 1477 GCCGGC [GA]^CCGG[CT]
BstEII 2729 GGTGACC G^GT[ACGT]ACC
BstPI 2729 GGTGACC G^GT[ACGT]ACC
Bsu36I 325 CCTTAGG CC^T[ACGT]AGG
CfoI 130 GCGC GCG^C
Cfr10I 1477 GCCGGC [GA]^CCGG[CT]
Eco81I 325 CCTTAGG CC^T[ACGT]AGG
EcoO65I 2729 GGTGACC G^GT[ACGT]ACC
EcoT22I 1530 ATGCAT ATGCA^T
HgaI 2138 GCGTC GACGC(5/10)
HhaI 130 GCGC GCG^C
HinP1I 130 GCGC G^CGC
MfeI 29 CAATTG C^AATTG
MunI 29 CAATTG C^AATTG
NaeI 1477 GCCGGC GCC^GGC
NgoMIV 1477 GCCGGC G^CCGGC
NsiI 1530 ATGCAT ATGCA^T
PflFI 1648 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 212 CCAATACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3028 CACGTG CAC^GTG
PmlI 3028 CACGTG CAC^GTG
PshAI 1571 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 3014 CAGCTG CAG^CTG
SbfI 2556 CCTGCAGG CCTGCA^GG
SexAI 54 ACCAGGT A^CC[AT]GGT
SpeI 1162 ACTAGT A^CTAGT
Sse8387I 2556 CCTGCAGG CCTGCA^GG
Tth111I 1648 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 212 CCAATACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1336 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 2103 GTGCAC G^TGCAC
3277 GTGCAC
ApaI 1319 GGGCCC GGGCC^C
2414 GGGCCC
ApaLI 2103 GTGCAC G^TGCAC
3277 GTGCAC
AvrII 699 CCTAGG C^CTAGG
3997 CCTAGG
BbsI 3901 GTCTTC GAAGAC(2/6)
3938 GTCTTC
BbvCI 113 CCTCAGC CCTCAGC(-5/-2)
4061 GCTGAGG
BcnI 164 CCGGG CC^[GC]GG
2507 CCCGG
BglI 731 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1321 GCCCCAGCGGC
BglII 1066 AGATCT A^GATCT
2006 AGATCT
BlnI 699 CCTAGG C^CTAGG
3997 CCTAGG
BpuAI 3901 GTCTTC GAAGAC(2/6)
3938 GTCTTC
Bsp1407I 464 TGTACA T^GTACA
4256 TGTACA
BsrGI 464 TGTACA T^GTACA
4256 TGTACA
DrdI 3836 GACAACTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4167 GACTGCTGGGTC
NciI 164 CCGGG CC^[GC]GG
2507 CCCGG
NcoI 1724 CCATGG C^CATGG
3682 CCATGG
NdeI 743 CATATG CA^TATG
4018 CATATG
NspI 814 GCATGT [GA]CATG^[CT]
2280 ACATGT
PpuMI 1576 AGGTCCC [GA]G^G[AT]CC[CT]
4065 AGGACCC
SapI 1662 GCTCTTC GCTCTTC(1/4)
1794 GCTCTTC
ScaI 259 AGTACT AGT^ACT
410 AGTACT
SspBI 464 TGTACA T^GTACA
4256 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
Acc65I AccI AciI AfaI AflIII
AluI AlwI AlwNI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII