##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccIII AclI AgeI ApaI AscI
AspI AviII BcgI BciVI BsaWI
BseAI BsiWI BspEI Bst1107I BstEII
BstPI BstZ17I CpoI DrdI EcoNI
EcoO65I EcoRV FseI FspI HpaI
KspI MluI MroI NaeI NdeI
NgoMIV NruI PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SgrAI
SnaBI SpeI Sse8387I Tth111I XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 724 GACGTC GACGT^C
Acc65I 4854 GGTACC G^GTACC
AhdI 4935 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3501 GTGCAC G^TGCAC
AlwNI 30 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 3864 AGCGCT AGC^GCT
ApaLI 3501 GTGCAC G^TGCAC
Asp700I 2336 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4854 GGTACC G^GTACC
AspEI 4935 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 672 GGATCC G^GATCC
BglII 1105 AGATCT A^GATCT
BlpI 515 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 515 GCTCAGC GC^T[ACGT]AGC
BsmBI 38 GAGACG CGTCTC(1/5)
Bsp1407I 1790 TGTACA T^GTACA
BspDI 500 ATCGAT AT^CGAT
BspHI 3031 TCATGA T^CATGA
BspLU11I 2385 ACATGT A^CATGT
BspMI 1606 ACCTGC ACCTGC(4/8)
BsrFI 321 ACCGGC [GA]^CCGG[CT]
BsrGI 1790 TGTACA T^GTACA
BssHII 417 GCGCGC G^CGCGC
BssSI 2003 CTCGTG CACGAG(-5/-1)
BstAPI 3325 GCATTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3457 TTCGAA TT^CGAA
BstXI 399 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 515 GCTCAGC GC^T[ACGT]AGC
Cfr10I 321 ACCGGC [GA]^CCGG[CT]
ClaI 500 ATCGAT AT^CGAT
DraIII 4956 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4935 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3864 AGCGCT AGC^GCT
KpnI 4854 GGTACC GGTAC^C
NcoI 399 CCATGG C^CATGG
NheI 5100 GCTAGC G^CTAGC
NotI 579 GCGGCCGC GC^GGCCGC
NspV 3457 TTCGAA TT^CGAA
RcaI 3031 TCATGA T^CATGA
SacI 918 GAGCTC GAGCT^C
SapI 1535 GAAGAGC GCTCTTC(1/4)
SexAI 4850 ACCTGGT A^CC[AT]GGT
SfiI 173 GGCCTGTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3457 TTCGAA TT^CGAA
SphI 1796 GCATGC GCATG^C
SspBI 1790 TGTACA T^GTACA
SspI 2822 AATATT AAT^ATT
StuI 5006 AGGCCT AGG^CCT
SwaI 4240 ATTTAAAT ATTT^AAAT
XmnI 2336 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AflII 4233 CTTAAG C^TTAAG
4989 CTTAAG
AocI 4518 CCTGAGG CC^T[ACGT]AGG
5140 CCTTAGG
AseI 1973 ATTAAT AT^TAAT
2687 ATTAAT
AsnI 1973 ATTAAT AT^TAAT
2687 ATTAAT
AvrII 85 CCTAGG C^CTAGG
126 CCTAGG
BalI 1119 TGGCCA TGG^CCA
3771 TGGCCA
BbrPI 1932 CACGTG CAC^GTG
2893 CACGTG
BbvCI 2832 CCTCAGC CCTCAGC(-5/-2)
167 GCTGAGG
BclI 1000 TGATCA T^GATCA
1854 TGATCA
BfrI 4233 CTTAAG C^TTAAG
4989 CTTAAG
BlnI 85 CCTAGG C^CTAGG
126 CCTAGG
BsaAI 1932 CACGTG [CT]AC^GT[GA]
2893 CACGTG
BsaBI 1306 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1451 GATGTTCATC
BsaI 479 GAGACC GGTCTC(1/5)
1824 GAGACC
BsrBI 513 CCGCTC CCGCTC(-3/-3)
577 GAGCGG
BsrDI 3472 CATTGC GCAATG(2/0)
4880 CATTGC
Bsu36I 4518 CCTGAGG CC^T[ACGT]AGG
5140 CCTTAGG
DraI 4241 TTTAAA TTT^AAA
4724 TTTAAA
DsaI 399 CCATGG C^C[GA][CT]GG
593 CCACGG
EagI 422 CGGCCG C^GGCCG
580 CGGCCG
EclXI 422 CGGCCG C^GGCCG
580 CGGCCG
Eco52I 422 CGGCCG C^GGCCG
580 CGGCCG
Eco81I 4518 CCTGAGG CC^T[ACGT]AGG
5140 CCTTAGG
EcoRI 1501 GAATTC G^AATTC
2717 GAATTC
EcoT22I 4356 ATGCAT ATGCA^T
4500 ATGCAT
FbaI 1000 TGATCA T^GATCA
1854 TGATCA
HincII 3748 GTTGAC GT[CT]^[GA]AC
5018 GTTGAC
HindII 3748 GTTGAC GT[CT]^[GA]AC
5018 GTTGAC
MamI 1306 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1451 GATGTTCATC
MluNI 1119 TGGCCA TGG^CCA
3771 TGGCCA
MscI 1119 TGGCCA TGG^CCA
3771 TGGCCA
NsiI 4356 ATGCAT ATGCA^T
4500 ATGCAT
PflMI 1340 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3221 CCAGCATTTGG
PmaCI 1932 CACGTG CAC^GTG
2893 CACGTG
PmlI 1932 CACGTG CAC^GTG
2893 CACGTG
PshBI 1973 ATTAAT AT^TAAT
2687 ATTAAT
PstI 6 CTGCAG CTGCA^G
372 CTGCAG
ScaI 764 AGTACT AGT^ACT
4762 AGTACT
SmaI 60 CCCGGG CCC^GGG
668 CCCGGG
Van91I 1340 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3221 CCAGCATTTGG
XmaCI 60 CCCGGG C^CCGGG
668 CCCGGG
XmaI 60 CCCGGG C^CCGGG
668 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII