##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI ApaI ApoI
AscI Asp718I AspEI AviII AvrII
BamHI BbrPI BbvCI BfrI BlnI
BsaAI BsaBI BseAI BsiWI BsmBI
BsmI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DraI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NdeI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI SacII
SalI SapI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 3087 GTCTAC GT^[AC][GT]AC
Aor51HI 136 AGCGCT AGC^GCT
AseI 386 ATTAAT AT^TAAT
AsnI 386 ATTAAT AT^TAAT
Asp700I 193 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 408 GTATCC GTATCC(6/5)
BclI 1201 TGATCA T^GATCA
BglII 2772 AGATCT A^GATCT
BsiEI 5 CGGCCG CG[GA][CT]^CG
BspHI 528 TCATGA T^CATGA
BsrFI 176 GCCGGC [GA]^CCGG[CT]
Cfr10I 176 GCCGGC [GA]^CCGG[CT]
CpoI 1694 CGGACCG CG^G[AT]CCG
DrdI 3486 GACATAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco47III 136 AGCGCT AGC^GCT
Eco52I 5 CGGCCG C^GGCCG
FbaI 1201 TGATCA T^GATCA
HincII 1456 GTTGAC GT[CT]^[GA]AC
HindII 1456 GTTGAC GT[CT]^[GA]AC
HindIII 1987 AAGCTT A^AGCTT
NaeI 176 GCCGGC GCC^GGC
NgoMIV 176 GCCGGC G^CCGGC
PaeR7I 1323 CTCGAG C^TCGAG
PshAI 3120 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 386 ATTAAT AT^TAAT
RcaI 528 TCATGA T^CATGA
RsrII 1694 CGGACCG CG^G[AT]CCG
ScaI 747 AGTACT AGT^ACT
SmaI 127 CCCGGG CCC^GGG
SphI 3512 GCATGC GCATG^C
SspI 3902 AATATT AAT^ATT
XbaI 2940 TCTAGA T^CTAGA
XhoI 1323 CTCGAG C^TCGAG
XmaCI 127 CCCGGG C^CCGGG
XmaI 127 CCCGGG C^CCGGG
XmnI 193 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 52 GGCGCC G[GA]^CG[CT]C
2803 GGCGCC
Alw44I 331 GTGCAC G^TGCAC
2601 GTGCAC
ApaLI 331 GTGCAC G^TGCAC
2601 GTGCAC
AspI 1411 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2617 GACTGTGTC
BbeI 52 GGCGCC GGCGC^C
2803 GGCGCC
BglI 176 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1590 GCCTCATGGGC
BsaHI 52 GGCGCC G[GA]^CG[CT]C
2803 GGCGCC
Bsp1407I 213 TGTACA T^GTACA
2846 TGTACA
BspLU11I 942 ACATGT A^CATGT
2859 ACATGT
BsrBI 355 CCGCTC CCGCTC(-3/-3)
1034 GAGCGG
BsrDI 3533 CATTGC GCAATG(2/0)
3750 GCAATG
BsrGI 213 TGTACA T^GTACA
2846 TGTACA
DraIII 1092 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1272 CACCCAGTG
EarI 1125 CTCTTC CTCTTC(1/4)
3307 CTCTTC
HgaI 1459 GACGC GACGC(5/10)
441 GCGTC
Hin1I 52 GGCGCC G[GA]^CG[CT]C
2803 GGCGCC
KasI 52 GGCGCC G^GCGCC
2803 GGCGCC
Ksp632I 1125 CTCTTC CTCTTC(1/4)
3307 CTCTTC
NarI 52 GGCGCC GG^CGCC
2803 GGCGCC
NheI 1316 GCTAGC G^CTAGC
2477 GCTAGC
PflFI 1411 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2617 GACTGTGTC
PflMI 1784 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3538 CCACTTGCTGG
SacI 1064 GAGCTC GAGCT^C
3586 GAGCTC
SfoI 52 GGCGCC GGC^GCC
2803 GGCGCC
SmlI 76 CTCAAG C^T[CT][GA]AG
1323 CTCGAG
SspBI 213 TGTACA T^GTACA
2846 TGTACA
Tth111I 1411 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2617 GACTGTGTC
Van91I 1784 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3538 CCACTTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcgI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII