################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AclI AcsI AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII BbvCI BciVI BclI BglII BsmI Bsp1407I BspDI BspHI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI Eam1105I EarI EcoO65I EcoRI EcoRV FbaI FseI FspI HpaI Ksp632I KspI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 687 GGTACC G^GTACC AccI 1333 GTCGAC GT^[AC][GT]AC AccIII 3043 TCCGGA T^CCGGA AflII 582 CTTAAG C^TTAAG ApaI 1997 GGGCCC GGGCC^C Asp718I 687 GGTACC G^GTACC AspI 2998 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 170 CCTAGG C^CTAGG BamHI 1462 GGATCC G^GATCC BbeI 731 GGCGCC GGCGC^C BbsI 1880 GAAGAC GAAGAC(2/6) BfrI 582 CTTAAG C^TTAAG BlnI 170 CCTAGG C^CTAGG BpuAI 1880 GAAGAC GAAGAC(2/6) BsaI 3054 GAGACC GGTCTC(1/5) BsaWI 3043 TCCGGA [AT]^CCGG[AT] BseAI 3043 TCCGGA T^CCGGA BsiEI 696 CGGCCG CG[GA][CT]^CG BsiWI 823 CGTACG C^GTACG BspEI 3043 TCCGGA T^CCGGA BssHII 1061 GCGCGC G^CGCGC BssSI 1501 CACGAG CACGAG(-5/-1) DraI 3267 TTTAAA TTT^AAA EagI 696 CGGCCG C^GGCCG EclXI 696 CGGCCG C^GGCCG Eco52I 696 CGGCCG C^GGCCG EcoT22I 663 ATGCAT ATGCA^T KasI 731 GGCGCC G^GCGCC KpnI 687 GGTACC GGTAC^C MfeI 3283 CAATTG C^AATTG MluI 820 ACGCGT A^CGCGT MroI 3043 TCCGGA T^CCGGA MunI 3283 CAATTG C^AATTG NarI 731 GGCGCC GG^CGCC NcoI 2296 CCATGG C^CATGG NsiI 663 ATGCAT ATGCA^T PflFI 2998 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2459 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1845 CTGCAG CTGCA^G SacI 2268 GAGCTC GAGCT^C SalI 1333 GTCGAC G^TCGAC SfcI 1845 CTGCAG C^T[GA][CT]AG SfoI 731 GGCGCC GGC^GCC Tsp509I 3284 AATT ^AATT Tth111I 2998 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2459 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AocI 1623 CCTGAGG CC^T[ACGT]AGG 2308 CCTGAGG Aor51HI 2394 AGCGCT AGC^GCT 2811 AGCGCT BbrPI 2565 CACGTG CAC^GTG 2604 CACGTG BcgI 739 GCAAGGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2993 GCAAAGACATCG BlpI 2265 GCTGAGC GC^T[ACGT]AGC 3117 GCTGAGC Bpu1102I 2265 GCTGAGC GC^T[ACGT]AGC 3117 GCTGAGC BsaBI 150 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1961 GATCCGCATC BsmBI 3179 CGTCTC CGTCTC(1/5) 288 GAGACG BspLU11I 1786 ACATGT A^CATGT 2660 ACATGT BsrDI 181 CATTGC GCAATG(2/0) 1109 CATTGC BstXI 496 CCAGGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1684 CCATTTGCATGG BstYI 1462 GGATCC [GA]^GATC[CT] 2404 GGATCT Bsu36I 1623 CCTGAGG CC^T[ACGT]AGG 2308 CCTGAGG CelII 2265 GCTGAGC GC^T[ACGT]AGC 3117 GCTGAGC DraIII 554 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2601 CACCACGTG Eco47III 2394 AGCGCT AGC^GCT 2811 AGCGCT Eco81I 1623 CCTGAGG CC^T[ACGT]AGG 2308 CCTGAGG HincII 1050 GTCAAC GT[CT]^[GA]AC 1333 GTCGAC HindII 1050 GTCAAC GT[CT]^[GA]AC 1333 GTCGAC HindIII 350 AAGCTT A^AGCTT 3263 AAGCTT MamI 150 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1961 GATCCGCATC MflI 1462 GGATCC [GA]^GATC[CT] 2404 GGATCT PmaCI 2565 CACGTG CAC^GTG 2604 CACGTG PmlI 2565 CACGTG CAC^GTG 2604 CACGTG PshAI 2721 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2975 GACCTCTGTC SfiI 1764 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1773 GGCCGCTGCGGCC SmaI 595 CCCGGG CCC^GGG 2934 CCCGGG TfiI 1619 GAATC G^A[AT]TC 2186 GATTC XcmI 1072 CCAACGCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3315 CCAGTCCCACTGTGG XhoII 1462 GGATCC [GA]^GATC[CT] 2404 GGATCT XmaCI 595 CCCGGG C^CCGGG 2934 CCCGGG XmaI 595 CCCGGG C^CCGGG 2934 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StuI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I