##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AcsI AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AviII BbvCI
BciVI BclI BglII BsmI Bsp1407I
BspDI BspHI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI Eam1105I EarI EcoO65I EcoRI
EcoRV FbaI FseI FspI HpaI
Ksp632I KspI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SapI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 687 GGTACC G^GTACC
AccI 1333 GTCGAC GT^[AC][GT]AC
AccIII 3043 TCCGGA T^CCGGA
AflII 582 CTTAAG C^TTAAG
ApaI 1997 GGGCCC GGGCC^C
Asp718I 687 GGTACC G^GTACC
AspI 2998 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 170 CCTAGG C^CTAGG
BamHI 1462 GGATCC G^GATCC
BbeI 731 GGCGCC GGCGC^C
BbsI 1880 GAAGAC GAAGAC(2/6)
BfrI 582 CTTAAG C^TTAAG
BlnI 170 CCTAGG C^CTAGG
BpuAI 1880 GAAGAC GAAGAC(2/6)
BsaI 3054 GAGACC GGTCTC(1/5)
BsaWI 3043 TCCGGA [AT]^CCGG[AT]
BseAI 3043 TCCGGA T^CCGGA
BsiEI 696 CGGCCG CG[GA][CT]^CG
BsiWI 823 CGTACG C^GTACG
BspEI 3043 TCCGGA T^CCGGA
BssHII 1061 GCGCGC G^CGCGC
BssSI 1501 CACGAG CACGAG(-5/-1)
DraI 3267 TTTAAA TTT^AAA
EagI 696 CGGCCG C^GGCCG
EclXI 696 CGGCCG C^GGCCG
Eco52I 696 CGGCCG C^GGCCG
EcoT22I 663 ATGCAT ATGCA^T
KasI 731 GGCGCC G^GCGCC
KpnI 687 GGTACC GGTAC^C
MfeI 3283 CAATTG C^AATTG
MluI 820 ACGCGT A^CGCGT
MroI 3043 TCCGGA T^CCGGA
MunI 3283 CAATTG C^AATTG
NarI 731 GGCGCC GG^CGCC
NcoI 2296 CCATGG C^CATGG
NsiI 663 ATGCAT ATGCA^T
PflFI 2998 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2459 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1845 CTGCAG CTGCA^G
SacI 2268 GAGCTC GAGCT^C
SalI 1333 GTCGAC G^TCGAC
SfcI 1845 CTGCAG C^T[GA][CT]AG
SfoI 731 GGCGCC GGC^GCC
Tsp509I 3284 AATT ^AATT
Tth111I 2998 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2459 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 1623 CCTGAGG CC^T[ACGT]AGG
2308 CCTGAGG
Aor51HI 2394 AGCGCT AGC^GCT
2811 AGCGCT
BbrPI 2565 CACGTG CAC^GTG
2604 CACGTG
BcgI 739 GCAAGGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2993 GCAAAGACATCG
BlpI 2265 GCTGAGC GC^T[ACGT]AGC
3117 GCTGAGC
Bpu1102I 2265 GCTGAGC GC^T[ACGT]AGC
3117 GCTGAGC
BsaBI 150 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1961 GATCCGCATC
BsmBI 3179 CGTCTC CGTCTC(1/5)
288 GAGACG
BspLU11I 1786 ACATGT A^CATGT
2660 ACATGT
BsrDI 181 CATTGC GCAATG(2/0)
1109 CATTGC
BstXI 496 CCAGGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1684 CCATTTGCATGG
BstYI 1462 GGATCC [GA]^GATC[CT]
2404 GGATCT
Bsu36I 1623 CCTGAGG CC^T[ACGT]AGG
2308 CCTGAGG
CelII 2265 GCTGAGC GC^T[ACGT]AGC
3117 GCTGAGC
DraIII 554 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2601 CACCACGTG
Eco47III 2394 AGCGCT AGC^GCT
2811 AGCGCT
Eco81I 1623 CCTGAGG CC^T[ACGT]AGG
2308 CCTGAGG
HincII 1050 GTCAAC GT[CT]^[GA]AC
1333 GTCGAC
HindII 1050 GTCAAC GT[CT]^[GA]AC
1333 GTCGAC
HindIII 350 AAGCTT A^AGCTT
3263 AAGCTT
MamI 150 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1961 GATCCGCATC
MflI 1462 GGATCC [GA]^GATC[CT]
2404 GGATCT
PmaCI 2565 CACGTG CAC^GTG
2604 CACGTG
PmlI 2565 CACGTG CAC^GTG
2604 CACGTG
PshAI 2721 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2975 GACCTCTGTC
SfiI 1764 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1773 GGCCGCTGCGGCC
SmaI 595 CCCGGG CCC^GGG
2934 CCCGGG
TfiI 1619 GAATC G^A[AT]TC
2186 GATTC
XcmI 1072 CCAACGCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3315 CCAGTCCCACTGTGG
XhoII 1462 GGATCC [GA]^GATC[CT]
2404 GGATCT
XmaCI 595 CCCGGG C^CCGGG
2934 CCCGGG
XmaI 595 CCCGGG C^CCGGG
2934 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I