##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
BamHI BanI BbeI BbrPI BciVI
BclI BglI BglII BlpI Bpu1102I
BsaBI BsaI BsiWI BspDI BsrFI
BssHII Bst1107I BstBI BstUI BstZ17I
CelII Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FbaI FseI HgaI HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI RsrII SacII SalI SexAI
SfiI SfoI SfuI SgrAI SwaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 728 GTCTAC GT^[AC][GT]AC
AccIII 1263 TCCGGA T^CCGGA
AclI 2397 AACGTT AA^CGTT
AflII 4078 CTTAAG C^TTAAG
AocI 2769 CCTGAGG CC^T[ACGT]AGG
ApaI 2984 GGGCCC GGGCC^C
AspI 3367 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1442 TGCGCA TGC^GCA
BbvCI 1771 GCTGAGG CCTCAGC(-5/-2)
BfrI 4078 CTTAAG C^TTAAG
BsaWI 1263 TCCGGA [AT]^CCGG[AT]
BseAI 1263 TCCGGA T^CCGGA
BsiEI 862 CGATCG CG[GA][CT]^CG
BsmBI 1008 GAGACG CGTCTC(1/5)
BspEI 1263 TCCGGA T^CCGGA
BspLU11I 2597 ACATGT A^CATGT
BsrBI 706 CCGCTC CCGCTC(-3/-3)
BsrDI 3408 GCAATG GCAATG(2/0)
BstAPI 2395 GCAACGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 654 GGTCACC G^GT[ACGT]ACC
BstPI 654 GGTCACC G^GT[ACGT]ACC
Bsu36I 2769 CCTGAGG CC^T[ACGT]AGG
DraI 4247 TTTAAA TTT^AAA
DraIII 922 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2769 CCTGAGG CC^T[ACGT]AGG
EcoO65I 654 GGTCACC G^GT[ACGT]ACC
EcoRI 1029 GAATTC G^AATTC
EcoT22I 4011 ATGCAT ATGCA^T
FspI 1442 TGCGCA TGC^GCA
HincII 598 GTCAAC GT[CT]^[GA]AC
HindII 598 GTCAAC GT[CT]^[GA]AC
MroI 1263 TCCGGA T^CCGGA
NdeI 202 CATATG CA^TATG
NsiI 4011 ATGCAT ATGCA^T
PaeR7I 2412 CTCGAG C^TCGAG
PflFI 3367 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2397 AACGTT AA^CGTT
PvuI 862 CGATCG CGAT^CG
SapI 2216 GCTCTTC GCTCTTC(1/4)
SbfI 711 CCTGCAGG CCTGCA^GG
ScaI 1513 AGTACT AGT^ACT
SnaBI 805 TACGTA TAC^GTA
SpeI 3040 ACTAGT A^CTAGT
Sse8387I 711 CCTGCAGG CCTGCA^GG
Tth111I 3367 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3379 TCTAGA T^CTAGA
XhoI 2412 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 2026 GACGTC GACGT^C
4087 GACGTC
AcyI 2026 GACGTC G[GA]^CG[CT]C
4087 GACGTC
AflIII 2342 ACACGT A^C[GA][CT]GT
2597 ACATGT
Alw44I 1177 GTGCAC G^TGCAC
1804 GTGCAC
ApaLI 1177 GTGCAC G^TGCAC
1804 GTGCAC
AvrII 1054 CCTAGG C^CTAGG
3238 CCTAGG
BalI 107 TGGCCA TGG^CCA
3667 TGGCCA
BcgI 2404 GCAGGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2546 GCAAGCTCATCG
BlnI 1054 CCTAGG C^CTAGG
3238 CCTAGG
BsaHI 2026 GACGTC G[GA]^CG[CT]C
4087 GACGTC
BsgI 2934 CTGCAC GTGCAG(16/14)
2787 GTGCAG
BsmI 2245 GAATGC GAATGC(1/-1)
815 GCATTC
EcoNI 2631 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3130 CCTTCGCAAGG
FauI 1678 CCCGC CCCGC(4/6)
2011 CCCGC
Hin1I 2026 GACGTC G[GA]^CG[CT]C
4087 GACGTC
MluNI 107 TGGCCA TGG^CCA
3667 TGGCCA
MscI 107 TGGCCA TGG^CCA
3667 TGGCCA
PvuII 289 CAGCTG CAG^CTG
2518 CAGCTG
SacI 1837 GAGCTC GAGCT^C
2574 GAGCTC
SmaI 1304 CCCGGG CCC^GGG
2440 CCCGGG
SphI 695 GCATGC GCATG^C
4009 GCATGC
SspI 2128 AATATT AAT^ATT
2854 AATATT
StuI 21 AGGCCT AGG^CCT
684 AGGCCT
XmaCI 1304 CCCGGG C^CCGGG
2440 CCCGGG
XmaI 1304 CCCGGG C^CCGGG
2440 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrGI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI