################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## Acc65I AccII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI BamHI BanI BbeI BbrPI BciVI BclI BglI BglII BlpI Bpu1102I BsaBI BsaI BsiWI BspDI BsrFI BssHII Bst1107I BstBI BstUI BstZ17I CelII Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FbaI FseI HgaI HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SwaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 728 GTCTAC GT^[AC][GT]AC AccIII 1263 TCCGGA T^CCGGA AclI 2397 AACGTT AA^CGTT AflII 4078 CTTAAG C^TTAAG AocI 2769 CCTGAGG CC^T[ACGT]AGG ApaI 2984 GGGCCC GGGCC^C AspI 3367 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1442 TGCGCA TGC^GCA BbvCI 1771 GCTGAGG CCTCAGC(-5/-2) BfrI 4078 CTTAAG C^TTAAG BsaWI 1263 TCCGGA [AT]^CCGG[AT] BseAI 1263 TCCGGA T^CCGGA BsiEI 862 CGATCG CG[GA][CT]^CG BsmBI 1008 GAGACG CGTCTC(1/5) BspEI 1263 TCCGGA T^CCGGA BspLU11I 2597 ACATGT A^CATGT BsrBI 706 CCGCTC CCGCTC(-3/-3) BsrDI 3408 GCAATG GCAATG(2/0) BstAPI 2395 GCAACGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 654 GGTCACC G^GT[ACGT]ACC BstPI 654 GGTCACC G^GT[ACGT]ACC Bsu36I 2769 CCTGAGG CC^T[ACGT]AGG DraI 4247 TTTAAA TTT^AAA DraIII 922 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2769 CCTGAGG CC^T[ACGT]AGG EcoO65I 654 GGTCACC G^GT[ACGT]ACC EcoRI 1029 GAATTC G^AATTC EcoT22I 4011 ATGCAT ATGCA^T FspI 1442 TGCGCA TGC^GCA HincII 598 GTCAAC GT[CT]^[GA]AC HindII 598 GTCAAC GT[CT]^[GA]AC MroI 1263 TCCGGA T^CCGGA NdeI 202 CATATG CA^TATG NsiI 4011 ATGCAT ATGCA^T PaeR7I 2412 CTCGAG C^TCGAG PflFI 3367 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 2397 AACGTT AA^CGTT PvuI 862 CGATCG CGAT^CG SapI 2216 GCTCTTC GCTCTTC(1/4) SbfI 711 CCTGCAGG CCTGCA^GG ScaI 1513 AGTACT AGT^ACT SnaBI 805 TACGTA TAC^GTA SpeI 3040 ACTAGT A^CTAGT Sse8387I 711 CCTGCAGG CCTGCA^GG Tth111I 3367 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3379 TCTAGA T^CTAGA XhoI 2412 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AatII 2026 GACGTC GACGT^C 4087 GACGTC AcyI 2026 GACGTC G[GA]^CG[CT]C 4087 GACGTC AflIII 2342 ACACGT A^C[GA][CT]GT 2597 ACATGT Alw44I 1177 GTGCAC G^TGCAC 1804 GTGCAC ApaLI 1177 GTGCAC G^TGCAC 1804 GTGCAC AvrII 1054 CCTAGG C^CTAGG 3238 CCTAGG BalI 107 TGGCCA TGG^CCA 3667 TGGCCA BcgI 2404 GCAGGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2546 GCAAGCTCATCG BlnI 1054 CCTAGG C^CTAGG 3238 CCTAGG BsaHI 2026 GACGTC G[GA]^CG[CT]C 4087 GACGTC BsgI 2934 CTGCAC GTGCAG(16/14) 2787 GTGCAG BsmI 2245 GAATGC GAATGC(1/-1) 815 GCATTC EcoNI 2631 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3130 CCTTCGCAAGG FauI 1678 CCCGC CCCGC(4/6) 2011 CCCGC Hin1I 2026 GACGTC G[GA]^CG[CT]C 4087 GACGTC MluNI 107 TGGCCA TGG^CCA 3667 TGGCCA MscI 107 TGGCCA TGG^CCA 3667 TGGCCA PvuII 289 CAGCTG CAG^CTG 2518 CAGCTG SacI 1837 GAGCTC GAGCT^C 2574 GAGCTC SmaI 1304 CCCGGG CCC^GGG 2440 CCCGGG SphI 695 GCATGC GCATG^C 4009 GCATGC SspI 2128 AATATT AAT^ATT 2854 AATATT StuI 21 AGGCCT AGG^CCT 684 AGGCCT XmaCI 1304 CCCGGG C^CCGGG 2440 CCCGGG XmaI 1304 CCCGGG C^CCGGG 2440 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrGI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI