##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccIII AclI AscI Asp700I BbeI
BciVI BclI BseAI BsiWI BsmBI
BspEI BsrBI EagI EclXI Eco52I
EcoRV EcoT22I FbaI FseI HindIII
HpaI KasI KspI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NotI NruI NsiI PacI PaeR7I
PmeI Psp1406I SacII SalI SbfI
ScaI SfiI SfoI SmaI SnaBI
Sse8387I SwaI XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 4013 GACGTC GACGT^C
Acc65I 115 GGTACC G^GTACC
AccII 3949 CGCG CG^CG
AgeI 1555 ACCGGT A^CCGGT
AhdI 2044 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1429 AGCGCT AGC^GCT
ApaI 3215 GGGCCC GGGCC^C
Asp718I 115 GGTACC G^GTACC
AspEI 2044 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3332 TGCGCA TGC^GCA
AvrII 2680 CCTAGG C^CTAGG
BamHI 925 GGATCC G^GATCC
BbrPI 4367 CACGTG CAC^GTG
BbvCI 1041 CCTCAGC CCTCAGC(-5/-2)
BcgI 3354 CGATCGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4345 AGATCT A^GATCT
BlnI 2680 CCTAGG C^CTAGG
BlpI 2107 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2107 GCTCAGC GC^T[ACGT]AGC
BsaBI 3851 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1555 ACCGGT [AT]^CCGG[AT]
BsiEI 3354 CGATCG CG[GA][CT]^CG
BspDI 3352 ATCGAT AT^CGAT
BspHI 3883 TCATGA T^CATGA
BssHII 3948 GCGCGC G^CGCGC
BssSI 3906 CTCGTG CACGAG(-5/-1)
Bst1107I 891 GTATAC GTA^TAC
BstAPI 1110 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1613 TTCGAA TT^CGAA
BstEII 255 GGTCACC G^GT[ACGT]ACC
BstPI 255 GGTCACC G^GT[ACGT]ACC
BstUI 3949 CGCG CG^CG
BstZ17I 891 GTATAC GTA^TAC
CelII 2107 GCTCAGC GC^T[ACGT]AGC
ClaI 3352 ATCGAT AT^CGAT
CpoI 2467 CGGACCG CG^G[AT]CCG
DrdI 853 GACAAAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2044 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1429 AGCGCT AGC^GCT
EcoO65I 255 GGTCACC G^GT[ACGT]ACC
EcoRI 4338 GAATTC G^AATTC
FspI 3332 TGCGCA TGC^GCA
KpnI 115 GGTACC GGTAC^C
MamI 3851 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 3949 CGCG CG^CG
NspV 1613 TTCGAA TT^CGAA
PinAI 1555 ACCGGT A^CCGGT
PmaCI 4367 CACGTG CAC^GTG
PmlI 4367 CACGTG CAC^GTG
PvuI 3354 CGATCG CGAT^CG
RcaI 3883 TCATGA T^CATGA
RsrII 2467 CGGACCG CG^G[AT]CCG
SacI 4221 GAGCTC GAGCT^C
SexAI 2606 ACCAGGT A^CC[AT]GGT
SfuI 1613 TTCGAA TT^CGAA
SgrAI 1554 CACCGGTG C[GA]^CCGG[CT]G
SpeI 598 ACTAGT A^CTAGT
SspI 1748 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 2230 AAATTC [GA]^AATT[CT]
4338 GAATTC
AflII 2949 CTTAAG C^TTAAG
3118 CTTAAG
Alw44I 2812 GTGCAC G^TGCAC
3614 GTGCAC
ApaLI 2812 GTGCAC G^TGCAC
3614 GTGCAC
ApoI 2230 AAATTC [GA]^AATT[CT]
4338 GAATTC
AseI 85 ATTAAT AT^TAAT
4681 ATTAAT
AsnI 85 ATTAAT AT^TAAT
4681 ATTAAT
AspI 1989 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3005 GACCTTGTC
BanI 115 GGTACC G^G[CT][GA]CC
4493 GGCACC
BfrI 2949 CTTAAG C^TTAAG
3118 CTTAAG
BsaAI 2878 CACGTA [CT]AC^GT[GA]
4367 CACGTG
BsgI 470 CTGCAC GTGCAG(16/14)
3891 CTGCAC
BsmI 3800 GAATGC GAATGC(1/-1)
2844 GCATTC
Bsp1407I 2432 TGTACA T^GTACA
2980 TGTACA
BsrDI 4262 CATTGC GCAATG(2/0)
1914 GCAATG
BsrFI 680 ACCGGC [GA]^CCGG[CT]
1555 ACCGGT
BsrGI 2432 TGTACA T^GTACA
2980 TGTACA
BstXI 1493 CCATCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1610 CCATTCGAATGG
Cfr10I 680 ACCGGC [GA]^CCGG[CT]
1555 ACCGGT
DraI 4713 TTTAAA TTT^AAA
4743 TTTAAA
FauI 4307 CCCGC CCCGC(4/6)
1407 GCGGG
NcoI 3576 CCATGG C^CATGG
3759 CCATGG
NdeI 896 CATATG CA^TATG
3936 CATATG
NheI 147 GCTAGC G^CTAGC
2668 GCTAGC
PflFI 1989 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3005 GACCTTGTC
PshAI 211 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3735 GACAAGTGTC
PshBI 85 ATTAAT AT^TAAT
4681 ATTAAT
SphI 2399 GCATGC GCATG^C
3952 GCATGC
SspBI 2432 TGTACA T^GTACA
2980 TGTACA
StuI 4043 AGGCCT AGG^CCT
4086 AGGCCT
Tth111I 1989 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3005 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI AspHI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII