################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BclI BlnI BlpI Bpu1102I BsaHI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I KasI KpnI KspI MluI MroI MvnI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfoI SfuI SgrAI Sse8387I SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 2704 GTCTAC GT^[AC][GT]AC AflII 2954 CTTAAG C^TTAAG ApaI 1232 GGGCCC GGGCC^C AspI 1945 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1333 TGGCCA TGG^CCA BcgI 270 GCACTTAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2208 GTATCC GTATCC(6/5) BfrI 2954 CTTAAG C^TTAAG BglI 3788 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 704 AGATCT A^GATCT BsaAI 460 TACGTA [CT]AC^GT[GA] BsaBI 2031 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 32 TCCGGT [AT]^CCGG[AT] BsmBI 3305 GAGACG CGTCTC(1/5) BsrFI 266 GCCGGC [GA]^CCGG[CT] BssSI 760 CTCGTG CACGAG(-5/-1) BstEII 1950 GGTCACC G^GT[ACGT]ACC BstPI 1950 GGTCACC G^GT[ACGT]ACC CfoI 385 GCGC GCG^C Cfr10I 266 GCCGGC [GA]^CCGG[CT] DraIII 3837 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1333 TGGCCA [CT]^GGCC[GA] EcoO65I 1950 GGTCACC G^GT[ACGT]ACC EcoRI 4065 GAATTC G^AATTC FauI 1633 GCGGG CCCGC(4/6) HhaI 385 GCGC GCG^C HinP1I 385 GCGC G^CGC HincII 997 GTTAAC GT[CT]^[GA]AC HindII 997 GTTAAC GT[CT]^[GA]AC HindIII 1717 AAGCTT A^AGCTT HpaI 997 GTTAAC GTT^AAC MaeII 461 ACGT A^CGT MamI 2031 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 606 CAATTG C^AATTG MluNI 1333 TGGCCA TGG^CCA MscI 1333 TGGCCA TGG^CCA MunI 606 CAATTG C^AATTG NaeI 266 GCCGGC GCC^GGC NgoMIV 266 GCCGGC G^CCGGC PflFI 1945 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3575 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 3401 GAGCTC GAGCT^C ScaI 529 AGTACT AGT^ACT SfiI 3787 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1043 CCCGGG CCC^GGG SnaBI 460 TACGTA TAC^GTA SpeI 3247 ACTAGT A^CTAGT Tth111I 1945 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2492 TCTAGA T^CTAGA XmaCI 1043 CCCGGG C^CCGGG XmaI 1043 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AvaI 1043 CCCGGG C^[CT]CG[GA]G 3908 CCCGAG BsmI 1520 GAATGC GAATGC(1/-1) 3851 GCATTC BsoBI 1043 CCCGGG C^[CT]CG[GA]G 3908 CCCGAG Bsp1407I 2904 TGTACA T^GTACA 3006 TGTACA BspHI 836 TCATGA T^CATGA 3324 TCATGA BsrGI 2904 TGTACA T^GTACA 3006 TGTACA BstAPI 1061 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1860 GCAATCACTGC BstXI 3230 CCATCGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3531 CCATTGCTGTGG EcoNI 1189 CCTTTAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2876 CCTGACAAAGG HgaI 4 GACGC GACGC(5/10) 3307 GACGC NdeI 20 CATATG CA^TATG 922 CATATG RcaI 836 TCATGA T^CATGA 3324 TCATGA SapI 4039 GAAGAGC GCTCTTC(1/4) 2683 GCTCTTC SexAI 1946 ACCAGGT A^CC[AT]GGT 2534 ACCAGGT SphI 221 GCATGC GCATG^C 1444 GCATGC SspBI 2904 TGTACA T^GTACA 3006 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII