##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbvCI
BclI BlnI BlpI Bpu1102I BsaHI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I KasI KpnI KspI MluI
MroI MvnI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfoI SfuI SgrAI Sse8387I
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 2704 GTCTAC GT^[AC][GT]AC
AflII 2954 CTTAAG C^TTAAG
ApaI 1232 GGGCCC GGGCC^C
AspI 1945 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1333 TGGCCA TGG^CCA
BcgI 270 GCACTTAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2208 GTATCC GTATCC(6/5)
BfrI 2954 CTTAAG C^TTAAG
BglI 3788 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 704 AGATCT A^GATCT
BsaAI 460 TACGTA [CT]AC^GT[GA]
BsaBI 2031 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 32 TCCGGT [AT]^CCGG[AT]
BsmBI 3305 GAGACG CGTCTC(1/5)
BsrFI 266 GCCGGC [GA]^CCGG[CT]
BssSI 760 CTCGTG CACGAG(-5/-1)
BstEII 1950 GGTCACC G^GT[ACGT]ACC
BstPI 1950 GGTCACC G^GT[ACGT]ACC
CfoI 385 GCGC GCG^C
Cfr10I 266 GCCGGC [GA]^CCGG[CT]
DraIII 3837 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1333 TGGCCA [CT]^GGCC[GA]
EcoO65I 1950 GGTCACC G^GT[ACGT]ACC
EcoRI 4065 GAATTC G^AATTC
FauI 1633 GCGGG CCCGC(4/6)
HhaI 385 GCGC GCG^C
HinP1I 385 GCGC G^CGC
HincII 997 GTTAAC GT[CT]^[GA]AC
HindII 997 GTTAAC GT[CT]^[GA]AC
HindIII 1717 AAGCTT A^AGCTT
HpaI 997 GTTAAC GTT^AAC
MaeII 461 ACGT A^CGT
MamI 2031 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 606 CAATTG C^AATTG
MluNI 1333 TGGCCA TGG^CCA
MscI 1333 TGGCCA TGG^CCA
MunI 606 CAATTG C^AATTG
NaeI 266 GCCGGC GCC^GGC
NgoMIV 266 GCCGGC G^CCGGC
PflFI 1945 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3575 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3401 GAGCTC GAGCT^C
ScaI 529 AGTACT AGT^ACT
SfiI 3787 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1043 CCCGGG CCC^GGG
SnaBI 460 TACGTA TAC^GTA
SpeI 3247 ACTAGT A^CTAGT
Tth111I 1945 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2492 TCTAGA T^CTAGA
XmaCI 1043 CCCGGG C^CCGGG
XmaI 1043 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaI 1043 CCCGGG C^[CT]CG[GA]G
3908 CCCGAG
BsmI 1520 GAATGC GAATGC(1/-1)
3851 GCATTC
BsoBI 1043 CCCGGG C^[CT]CG[GA]G
3908 CCCGAG
Bsp1407I 2904 TGTACA T^GTACA
3006 TGTACA
BspHI 836 TCATGA T^CATGA
3324 TCATGA
BsrGI 2904 TGTACA T^GTACA
3006 TGTACA
BstAPI 1061 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1860 GCAATCACTGC
BstXI 3230 CCATCGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3531 CCATTGCTGTGG
EcoNI 1189 CCTTTAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2876 CCTGACAAAGG
HgaI 4 GACGC GACGC(5/10)
3307 GACGC
NdeI 20 CATATG CA^TATG
922 CATATG
RcaI 836 TCATGA T^CATGA
3324 TCATGA
SapI 4039 GAAGAGC GCTCTTC(1/4)
2683 GCTCTTC
SexAI 1946 ACCAGGT A^CC[AT]GGT
2534 ACCAGGT
SphI 221 GCATGC GCATG^C
1444 GCATGC
SspBI 2904 TGTACA T^GTACA
3006 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII