##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Aor51HI AscI AseI
AsnI Asp718I AvrII BamHI BbrPI
BcgI BfrI BglII BlnI BsaAI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI Eco47III
EcoNI EcoRV FseI HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AhdI 1878 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1333 GGGCCC GGGCC^C
AspEI 1878 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2408 TGCGCA TGC^GCA
BalI 3613 TGGCCA TGG^CCA
BbeI 2440 GGCGCC GGCGC^C
BlpI 2527 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2527 GCTAAGC GC^T[ACGT]AGC
BsiEI 3483 CGGCCG CG[GA][CT]^CG
BsmBI 3222 GAGACG CGTCTC(1/5)
BspHI 2153 TCATGA T^CATGA
BsrDI 1415 GCAATG GCAATG(2/0)
BssSI 356 CTCGTG CACGAG(-5/-1)
CelII 2527 GCTAAGC GC^T[ACGT]AGC
DraI 3921 TTTAAA TTT^AAA
DraIII 3762 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 941 GACATTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3483 CGGCCG C^GGCCG
Eam1105I 1878 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3483 CGGCCG C^GGCCG
Eco52I 3483 CGGCCG C^GGCCG
EcoRI 541 GAATTC G^AATTC
EcoT22I 4175 ATGCAT ATGCA^T
FspI 2408 TGCGCA TGC^GCA
HindIII 1736 AAGCTT A^AGCTT
KasI 2440 GGCGCC G^GCGCC
MluNI 3613 TGGCCA TGG^CCA
MscI 3613 TGGCCA TGG^CCA
NarI 2440 GGCGCC GG^CGCC
NsiI 4175 ATGCAT ATGCA^T
PpuMI 3546 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 513 CAGCTG CAG^CTG
RcaI 2153 TCATGA T^CATGA
SapI 349 GAAGAGC GCTCTTC(1/4)
ScaI 2507 AGTACT AGT^ACT
SfoI 2440 GGCGCC GGC^GCC
SmaI 46 CCCGGG CCC^GGG
SphI 927 GCATGC GCATG^C
XmaCI 46 CCCGGG C^CCGGG
XmaI 46 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 26 CGCG CG^CG
4056 CGCG
Alw44I 1628 GTGCAC G^TGCAC
3796 GTGCAC
AocI 67 CCTGAGG CC^T[ACGT]AGG
2515 CCTGAGG
ApaLI 1628 GTGCAC G^TGCAC
3796 GTGCAC
Asp700I 938 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2872 GAAAATGTTC
AspI 1573 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2368 GACCACGTC
BbsI 938 GAAGAC GAAGAC(2/6)
3414 GTCTTC
BciVI 589 GGATAC GTATCC(6/5)
1021 GGATAC
BpuAI 938 GAAGAC GAAGAC(2/6)
3414 GTCTTC
BsaWI 1606 TCCGGT [AT]^CCGG[AT]
2653 ACCGGA
BsmI 1931 GAATGC GAATGC(1/-1)
3635 GCATTC
BspMI 79 GCAGGT ACCTGC(4/8)
1165 GCAGGT
BsrBI 93 GAGCGG CCGCTC(-3/-3)
1123 GAGCGG
BstEII 244 GGTGACC G^GT[ACGT]ACC
2083 GGTTACC
BstPI 244 GGTGACC G^GT[ACGT]ACC
2083 GGTTACC
BstUI 26 CGCG CG^CG
4056 CGCG
Bsu36I 67 CCTGAGG CC^T[ACGT]AGG
2515 CCTGAGG
DraII 500 AGGCCCC [GA]G^G[ACGT]CC[CT]
3546 AGGACCC
Eco81I 67 CCTGAGG CC^T[ACGT]AGG
2515 CCTGAGG
EcoO109I 500 AGGCCCC [GA]G^G[ACGT]CC[CT]
3546 AGGACCC
EcoO65I 244 GGTGACC G^GT[ACGT]ACC
2083 GGTTACC
HincII 1360 GTTGAC GT[CT]^[GA]AC
1771 GTCAAC
HindII 1360 GTTGAC GT[CT]^[GA]AC
1771 GTCAAC
MspA1I 513 CAGCTG C[AC]G^C[GT]G
790 CAGCGG
MvnI 26 CGCG CG^CG
4056 CGCG
NaeI 3078 GCCGGC GCC^GGC
3481 GCCGGC
NgoMIV 3078 GCCGGC G^CCGGC
3481 GCCGGC
PflFI 1573 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2368 GACCACGTC
SmlI 811 CTCAAG C^T[CT][GA]AG
3313 CTCAAG
Tth111I 1573 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2368 GACCACGTC
XmnI 938 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2872 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PshAI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII