##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccIII AgeI ApaI AscI
AseI AsnI AspI BbvCI BglII
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BstBI
BstEII BstPI ClaI DrdI EcoO65I
EcoRI EcoRV EcoT22I FseI HincII
HindII HpaI MluI MroI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI PvuI SacI SalI SapI
SfiI SfuI SmaI SpeI SphI
SspBI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 4458 GGTACC G^GTACC
AclI 2525 AACGTT AA^CGTT
AflIII 3282 ACGTGT A^C[GA][CT]GT
AhdI 128 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3322 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4458 GGTACC G^GTACC
AspEI 128 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4453 CCTAGG C^CTAGG
BamHI 1203 GGATCC G^GATCC
BbrPI 3281 CACGTG CAC^GTG
BcgI 1536 CGACGACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2881 TGATCA T^GATCA
BglI 4258 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4453 CCTAGG C^CTAGG
BsaBI 2046 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssHII 152 GCGCGC G^CGCGC
Bst1107I 3915 GTATAC GTA^TAC
BstAPI 11 GCACTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3915 GTATAC GTA^TAC
CpoI 26 CGGTCCG CG^G[AT]CCG
Eam1105I 128 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2881 TGATCA T^GATCA
KpnI 4458 GGTACC GGTAC^C
MamI 2046 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 907 CAATTG C^AATTG
MunI 907 CAATTG C^AATTG
NcoI 437 CCATGG C^CATGG
NdeI 4017 CATATG CA^TATG
PmaCI 3281 CACGTG CAC^GTG
PmlI 3281 CACGTG CAC^GTG
Psp1406I 2525 AACGTT AA^CGTT
RsrII 26 CGGTCCG CG^G[AT]CCG
SbfI 4509 CCTGCAGG CCTGCA^GG
ScaI 4587 AGTACT AGT^ACT
SgrAI 1530 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 4533 TACGTA TAC^GTA
Sse8387I 4509 CCTGCAGG CCTGCA^GG
StuI 3476 AGGCCT AGG^CCT
XmnI 3322 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2389 GTCTAC GT^[AC][GT]AC
3915 GTATAC
AflII 1078 CTTAAG C^TTAAG
3674 CTTAAG
Aor51HI 747 AGCGCT AGC^GCT
1629 AGCGCT
AviII 51 TGCGCA TGC^GCA
3143 TGCGCA
BciVI 4355 GTATCC GTATCC(6/5)
4502 GTATCC
BfrI 1078 CTTAAG C^TTAAG
3674 CTTAAG
BsiEI 31 CGGCCG CG[GA][CT]^CG
138 CGGCCG
BsmBI 234 GAGACG CGTCTC(1/5)
401 GAGACG
BstXI 4034 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4276 CCAAAACCCTGG
BstYI 1203 GGATCC [GA]^GATC[CT]
1995 GGATCT
DraI 3517 TTTAAA TTT^AAA
4656 TTTAAA
EagI 31 CGGCCG C^GGCCG
138 CGGCCG
EclXI 31 CGGCCG C^GGCCG
138 CGGCCG
Eco47III 747 AGCGCT AGC^GCT
1629 AGCGCT
Eco52I 31 CGGCCG C^GGCCG
138 CGGCCG
FspI 51 TGCGCA TGC^GCA
3143 TGCGCA
MflI 1203 GGATCC [GA]^GATC[CT]
1995 GGATCT
PflMI 310 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1365 CCAGTCCCTGG
PshAI 3029 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3399 GACAAATGTC
SexAI 824 ACCAGGT A^CC[AT]GGT
3429 ACCAGGT
SspI 1574 AATATT AAT^ATT
3874 AATATT
Van91I 310 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1365 CCAGTCCCTGG
XbaI 2816 TCTAGA T^CTAGA
3347 TCTAGA
XcmI 1340 CCAATGCCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2831 CCATTTGCAAAATGG
XhoII 1203 GGATCC [GA]^GATC[CT]
1995 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RcaI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I