##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AclI AhdI AocI
Aor51HI AscI AspEI AviII AvrII
BclI BglII BlnI BseAI BsiWI
BspDI BspEI BspHI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FauI FbaI FseI FspI
KspI MluI MroI NaeI NgoMIV
NotI NruI NspV PacI PmeI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 2936 GGTACC G^GTACC
AccII 3890 CGCG CG^CG
AcyI 572 GGCGCC G[GA]^CG[CT]C
AflII 2600 CTTAAG C^TTAAG
AgeI 3055 ACCGGT A^CCGGT
Alw44I 4144 GTGCAC G^TGCAC
ApaI 4118 GGGCCC GGGCC^C
ApaLI 4144 GTGCAC G^TGCAC
Asp718I 2936 GGTACC G^GTACC
AspI 696 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 572 GGCGCC GGCGC^C
BbrPI 1472 CACGTG CAC^GTG
BfrI 2600 CTTAAG C^TTAAG
BsaAI 1472 CACGTG [CT]AC^GT[GA]
BsaBI 384 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 572 GGCGCC G[GA]^CG[CT]C
BsaI 3690 GGTCTC GGTCTC(1/5)
BsiEI 1794 CGGTCG CG[GA][CT]^CG
BsmBI 1407 CGTCTC CGTCTC(1/5)
Bsp1407I 2634 TGTACA T^GTACA
BspLU11I 2694 ACATGT A^CATGT
BsrBI 2207 GAGCGG CCGCTC(-3/-3)
BsrFI 3055 ACCGGT [GA]^CCGG[CT]
BsrGI 2634 TGTACA T^GTACA
BssSI 1659 CACGAG CACGAG(-5/-1)
BstAPI 1741 GCATCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3890 CGCG CG^CG
Cfr10I 3055 ACCGGT [GA]^CCGG[CT]
DraIII 2783 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 1787 GATATC GAT^ATC
EcoT22I 2744 ATGCAT ATGCA^T
HgaI 1406 GCGTC GACGC(5/10)
Hin1I 572 GGCGCC G[GA]^CG[CT]C
HindIII 4178 AAGCTT A^AGCTT
KasI 572 GGCGCC G^GCGCC
KpnI 2936 GGTACC GGTAC^C
MamI 384 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 4137 CATCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 3890 CGCG CG^CG
NarI 572 GGCGCC GG^CGCC
NcoI 3675 CCATGG C^CATGG
NdeI 4500 CATATG CA^TATG
NsiI 2744 ATGCAT ATGCA^T
PaeR7I 3942 CTCGAG C^TCGAG
PflFI 696 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3055 ACCGGT A^CCGGT
PmaCI 1472 CACGTG CAC^GTG
PmlI 1472 CACGTG CAC^GTG
SexAI 3498 ACCTGGT A^CC[AT]GGT
SfiI 3065 GGCCCTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 572 GGCGCC GGC^GCC
SspBI 2634 TGTACA T^GTACA
SwaI 4539 ATTTAAAT ATTT^AAAT
Tth111I 696 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1783 TCTAGA T^CTAGA
XcmI 109 CCAGAGTAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3942 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2690 GTCTAC GT^[AC][GT]AC
3976 GTCTAC
AflIII 2694 ACATGT A^C[GA][CT]GT
3980 ACACGT
AseI 1349 ATTAAT AT^TAAT
4490 ATTAAT
AsnI 1349 ATTAAT AT^TAAT
4490 ATTAAT
BbsI 361 GAAGAC GAAGAC(2/6)
3835 GAAGAC
BbvCI 2858 CCTCAGC CCTCAGC(-5/-2)
874 GCTGAGG
BciVI 89 GTATCC GTATCC(6/5)
649 GTATCC
BcnI 443 CCGGG CC^[GC]GG
4140 CCCGG
BpuAI 361 GAAGAC GAAGAC(2/6)
3835 GAAGAC
BsaWI 158 ACCGGA [AT]^CCGG[AT]
3055 ACCGGT
BstXI 316 CCACTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3795 CCAGCTCTCTGG
CfoI 573 GCGC GCG^C
1372 GCGC
DraI 2531 TTTAAA TTT^AAA
4540 TTTAAA
DsaI 821 CCACGG C^C[GA][CT]GG
3675 CCATGG
HaeII 572 GGCGCC [GA]GCGC^[CT]
1371 GGCGCT
HhaI 573 GCGC GCG^C
1372 GCGC
HinP1I 573 GCGC G^CGC
1372 GCGC
HincII 2683 GTTAAC GT[CT]^[GA]AC
4253 GTTAAC
HindII 2683 GTTAAC GT[CT]^[GA]AC
4253 GTTAAC
HpaI 2683 GTTAAC GTT^AAC
4253 GTTAAC
MfeI 3 CAATTG C^AATTG
2221 CAATTG
MunI 3 CAATTG C^AATTG
2221 CAATTG
NciI 443 CCGGG CC^[GC]GG
4140 CCCGG
NheI 422 GCTAGC G^CTAGC
1447 GCTAGC
PpuMI 273 AGGACCT [GA]G^G[AT]CC[CT]
1942 AGGACCT
PshBI 1349 ATTAAT AT^TAAT
4490 ATTAAT
SacI 2736 GAGCTC GAGCT^C
2879 GAGCTC
SapI 2204 GAAGAGC GCTCTTC(1/4)
2881 GCTCTTC
SspI 1769 AATATT AAT^ATT
1924 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BalI BamHI BanI BanII
BbvI BcgI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI