################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII AccIII AclI AhdI AocI Aor51HI AscI AspEI AviII AvrII BclI BglII BlnI BseAI BsiWI BspDI BspEI BspHI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FauI FbaI FseI FspI KspI MluI MroI NaeI NgoMIV NotI NruI NspV PacI PmeI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 2936 GGTACC G^GTACC AccII 3890 CGCG CG^CG AcyI 572 GGCGCC G[GA]^CG[CT]C AflII 2600 CTTAAG C^TTAAG AgeI 3055 ACCGGT A^CCGGT Alw44I 4144 GTGCAC G^TGCAC ApaI 4118 GGGCCC GGGCC^C ApaLI 4144 GTGCAC G^TGCAC Asp718I 2936 GGTACC G^GTACC AspI 696 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 572 GGCGCC GGCGC^C BbrPI 1472 CACGTG CAC^GTG BfrI 2600 CTTAAG C^TTAAG BsaAI 1472 CACGTG [CT]AC^GT[GA] BsaBI 384 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 572 GGCGCC G[GA]^CG[CT]C BsaI 3690 GGTCTC GGTCTC(1/5) BsiEI 1794 CGGTCG CG[GA][CT]^CG BsmBI 1407 CGTCTC CGTCTC(1/5) Bsp1407I 2634 TGTACA T^GTACA BspLU11I 2694 ACATGT A^CATGT BsrBI 2207 GAGCGG CCGCTC(-3/-3) BsrFI 3055 ACCGGT [GA]^CCGG[CT] BsrGI 2634 TGTACA T^GTACA BssSI 1659 CACGAG CACGAG(-5/-1) BstAPI 1741 GCATCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 3890 CGCG CG^CG Cfr10I 3055 ACCGGT [GA]^CCGG[CT] DraIII 2783 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 1787 GATATC GAT^ATC EcoT22I 2744 ATGCAT ATGCA^T HgaI 1406 GCGTC GACGC(5/10) Hin1I 572 GGCGCC G[GA]^CG[CT]C HindIII 4178 AAGCTT A^AGCTT KasI 572 GGCGCC G^GCGCC KpnI 2936 GGTACC GGTAC^C MamI 384 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 4137 CATCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 3890 CGCG CG^CG NarI 572 GGCGCC GG^CGCC NcoI 3675 CCATGG C^CATGG NdeI 4500 CATATG CA^TATG NsiI 2744 ATGCAT ATGCA^T PaeR7I 3942 CTCGAG C^TCGAG PflFI 696 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3055 ACCGGT A^CCGGT PmaCI 1472 CACGTG CAC^GTG PmlI 1472 CACGTG CAC^GTG SexAI 3498 ACCTGGT A^CC[AT]GGT SfiI 3065 GGCCCTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 572 GGCGCC GGC^GCC SspBI 2634 TGTACA T^GTACA SwaI 4539 ATTTAAAT ATTT^AAAT Tth111I 696 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1783 TCTAGA T^CTAGA XcmI 109 CCAGAGTAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3942 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 2690 GTCTAC GT^[AC][GT]AC 3976 GTCTAC AflIII 2694 ACATGT A^C[GA][CT]GT 3980 ACACGT AseI 1349 ATTAAT AT^TAAT 4490 ATTAAT AsnI 1349 ATTAAT AT^TAAT 4490 ATTAAT BbsI 361 GAAGAC GAAGAC(2/6) 3835 GAAGAC BbvCI 2858 CCTCAGC CCTCAGC(-5/-2) 874 GCTGAGG BciVI 89 GTATCC GTATCC(6/5) 649 GTATCC BcnI 443 CCGGG CC^[GC]GG 4140 CCCGG BpuAI 361 GAAGAC GAAGAC(2/6) 3835 GAAGAC BsaWI 158 ACCGGA [AT]^CCGG[AT] 3055 ACCGGT BstXI 316 CCACTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3795 CCAGCTCTCTGG CfoI 573 GCGC GCG^C 1372 GCGC DraI 2531 TTTAAA TTT^AAA 4540 TTTAAA DsaI 821 CCACGG C^C[GA][CT]GG 3675 CCATGG HaeII 572 GGCGCC [GA]GCGC^[CT] 1371 GGCGCT HhaI 573 GCGC GCG^C 1372 GCGC HinP1I 573 GCGC G^CGC 1372 GCGC HincII 2683 GTTAAC GT[CT]^[GA]AC 4253 GTTAAC HindII 2683 GTTAAC GT[CT]^[GA]AC 4253 GTTAAC HpaI 2683 GTTAAC GTT^AAC 4253 GTTAAC MfeI 3 CAATTG C^AATTG 2221 CAATTG MunI 3 CAATTG C^AATTG 2221 CAATTG NciI 443 CCGGG CC^[GC]GG 4140 CCCGG NheI 422 GCTAGC G^CTAGC 1447 GCTAGC PpuMI 273 AGGACCT [GA]G^G[AT]CC[CT] 1942 AGGACCT PshBI 1349 ATTAAT AT^TAAT 4490 ATTAAT SacI 2736 GAGCTC GAGCT^C 2879 GAGCTC SapI 2204 GAAGAGC GCTCTTC(1/4) 2881 GCTCTTC SspI 1769 AATATT AAT^ATT 1924 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanI BanII BbvI BcgI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI