##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI AocI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII BfrI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrGI Bst1107I
BstBI BstZ17I Bsu36I ClaI DraI
DrdI Eam1105I Eco81I EcoRI EcoRV
FseI FspI HindIII HpaI KpnI
MfeI MluI MroI MunI NcoI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
SbfI SfiI SfuI SnaBI Sse8387I
SspBI SspI SwaI TfiI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1627 GACGTC GACGT^C
AvrII 2164 CCTAGG C^CTAGG
BamHI 1200 GGATCC G^GATCC
BbeI 2183 GGCGCC GGCGC^C
BciVI 90 GTATCC GTATCC(6/5)
BglI 2013 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1659 AGATCT A^GATCT
BlnI 2164 CCTAGG C^CTAGG
BlpI 1720 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1720 GCTGAGC GC^T[ACGT]AGC
BsaBI 2257 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2706 TCCGGT [AT]^CCGG[AT]
BsmI 939 GAATGC GAATGC(1/-1)
BspHI 2558 TCATGA T^CATGA
BsrBI 2112 CCGCTC CCGCTC(-3/-3)
BssHII 2452 GCGCGC G^CGCGC
BstEII 1612 GGTCACC G^GT[ACGT]ACC
BstPI 1612 GGTCACC G^GT[ACGT]ACC
CelII 1720 GCTGAGC GC^T[ACGT]AGC
CpoI 1119 CGGACCG CG^G[AT]CCG
DraIII 2118 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1063 CCTCTCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1612 GGTCACC G^GT[ACGT]ACC
EcoT22I 2483 ATGCAT ATGCA^T
KasI 2183 GGCGCC G^GCGCC
KspI 51 CCGCGG CCGC^GG
MamI 2257 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2183 GGCGCC GG^CGCC
NheI 1244 GCTAGC G^CTAGC
NotI 53 GCGGCCGC GC^GGCCGC
NsiI 2483 ATGCAT ATGCA^T
RcaI 2558 TCATGA T^CATGA
RsrII 1119 CGGACCG CG^G[AT]CCG
SacI 1439 GAGCTC GAGCT^C
SacII 51 CCGCGG CCGC^GG
SalI 44 GTCGAC G^TCGAC
SapI 1073 GAAGAGC GCTCTTC(1/4)
ScaI 549 AGTACT AGT^ACT
SexAI 1235 ACCAGGT A^CC[AT]GGT
SfoI 2183 GGCGCC GGC^GCC
SgrAI 1889 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1984 CCCGGG CCC^GGG
SpeI 400 ACTAGT A^CTAGT
SphI 2498 GCATGC GCATG^C
XbaI 469 TCTAGA T^CTAGA
XmaCI 1984 CCCGGG C^CCGGG
XmaI 1984 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 686 AAATTT [GA]^AATT[CT]
2689 AAATTC
Alw44I 1760 GTGCAC G^TGCAC
2829 GTGCAC
Aor51HI 1767 AGCGCT AGC^GCT
2620 AGCGCT
ApaLI 1760 GTGCAC G^TGCAC
2829 GTGCAC
ApoI 686 AAATTT [GA]^AATT[CT]
2689 AAATTC
BbrPI 2118 CACGTG CAC^GTG
2248 CACGTG
BcgI 769 CGATGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1098 GCATGGTGCTCG
BclI 873 TGATCA T^GATCA
930 TGATCA
BsaAI 2118 CACGTG [CT]AC^GT[GA]
2248 CACGTG
BsiEI 28 CGGCCG CG[GA][CT]^CG
54 CGGCCG
BsrDI 1741 CATTGC GCAATG(2/0)
3019 GCAATG
BstAPI 731 GCATACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1585 GCAGGTGGTGC
BstYI 1200 GGATCC [GA]^GATC[CT]
1659 AGATCT
DsaI 51 CCGCGG C^C[GA][CT]GG
2849 CCACGG
EagI 28 CGGCCG C^GGCCG
54 CGGCCG
EclXI 28 CGGCCG C^GGCCG
54 CGGCCG
Eco47III 1767 AGCGCT AGC^GCT
2620 AGCGCT
Eco52I 28 CGGCCG C^GGCCG
54 CGGCCG
FbaI 873 TGATCA T^GATCA
930 TGATCA
MflI 1200 GGATCC [GA]^GATC[CT]
1659 AGATCT
NdeI 140 CATATG CA^TATG
787 CATATG
PflMI 1003 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1093 CCAGCGCATGG
PmaCI 2118 CACGTG CAC^GTG
2248 CACGTG
PmlI 2118 CACGTG CAC^GTG
2248 CACGTG
PvuII 2174 CAGCTG CAG^CTG
2422 CAGCTG
SmlI 2056 CTCAAG C^T[CT][GA]AG
3032 CTCAAG
Van91I 1003 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1093 CCAGCGCATGG
XcmI 2014 CCAGGGAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2187 CCAAGTACATTCTGG
XhoII 1200 GGATCC [GA]^GATC[CT]
1659 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I