################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AviII BfrI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI DraI DrdI Eam1105I Eco81I EcoRI EcoRV FseI FspI HindIII HpaI KpnI MfeI MluI MroI MunI NcoI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI SbfI SfiI SfuI SnaBI Sse8387I SspBI SspI SwaI TfiI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1627 GACGTC GACGT^C AvrII 2164 CCTAGG C^CTAGG BamHI 1200 GGATCC G^GATCC BbeI 2183 GGCGCC GGCGC^C BciVI 90 GTATCC GTATCC(6/5) BglI 2013 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1659 AGATCT A^GATCT BlnI 2164 CCTAGG C^CTAGG BlpI 1720 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1720 GCTGAGC GC^T[ACGT]AGC BsaBI 2257 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2706 TCCGGT [AT]^CCGG[AT] BsmI 939 GAATGC GAATGC(1/-1) BspHI 2558 TCATGA T^CATGA BsrBI 2112 CCGCTC CCGCTC(-3/-3) BssHII 2452 GCGCGC G^CGCGC BstEII 1612 GGTCACC G^GT[ACGT]ACC BstPI 1612 GGTCACC G^GT[ACGT]ACC CelII 1720 GCTGAGC GC^T[ACGT]AGC CpoI 1119 CGGACCG CG^G[AT]CCG DraIII 2118 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 1063 CCTCTCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1612 GGTCACC G^GT[ACGT]ACC EcoT22I 2483 ATGCAT ATGCA^T KasI 2183 GGCGCC G^GCGCC KspI 51 CCGCGG CCGC^GG MamI 2257 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 2183 GGCGCC GG^CGCC NheI 1244 GCTAGC G^CTAGC NotI 53 GCGGCCGC GC^GGCCGC NsiI 2483 ATGCAT ATGCA^T RcaI 2558 TCATGA T^CATGA RsrII 1119 CGGACCG CG^G[AT]CCG SacI 1439 GAGCTC GAGCT^C SacII 51 CCGCGG CCGC^GG SalI 44 GTCGAC G^TCGAC SapI 1073 GAAGAGC GCTCTTC(1/4) ScaI 549 AGTACT AGT^ACT SexAI 1235 ACCAGGT A^CC[AT]GGT SfoI 2183 GGCGCC GGC^GCC SgrAI 1889 CACCGGCG C[GA]^CCGG[CT]G SmaI 1984 CCCGGG CCC^GGG SpeI 400 ACTAGT A^CTAGT SphI 2498 GCATGC GCATG^C XbaI 469 TCTAGA T^CTAGA XmaCI 1984 CCCGGG C^CCGGG XmaI 1984 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 686 AAATTT [GA]^AATT[CT] 2689 AAATTC Alw44I 1760 GTGCAC G^TGCAC 2829 GTGCAC Aor51HI 1767 AGCGCT AGC^GCT 2620 AGCGCT ApaLI 1760 GTGCAC G^TGCAC 2829 GTGCAC ApoI 686 AAATTT [GA]^AATT[CT] 2689 AAATTC BbrPI 2118 CACGTG CAC^GTG 2248 CACGTG BcgI 769 CGATGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1098 GCATGGTGCTCG BclI 873 TGATCA T^GATCA 930 TGATCA BsaAI 2118 CACGTG [CT]AC^GT[GA] 2248 CACGTG BsiEI 28 CGGCCG CG[GA][CT]^CG 54 CGGCCG BsrDI 1741 CATTGC GCAATG(2/0) 3019 GCAATG BstAPI 731 GCATACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1585 GCAGGTGGTGC BstYI 1200 GGATCC [GA]^GATC[CT] 1659 AGATCT DsaI 51 CCGCGG C^C[GA][CT]GG 2849 CCACGG EagI 28 CGGCCG C^GGCCG 54 CGGCCG EclXI 28 CGGCCG C^GGCCG 54 CGGCCG Eco47III 1767 AGCGCT AGC^GCT 2620 AGCGCT Eco52I 28 CGGCCG C^GGCCG 54 CGGCCG FbaI 873 TGATCA T^GATCA 930 TGATCA MflI 1200 GGATCC [GA]^GATC[CT] 1659 AGATCT NdeI 140 CATATG CA^TATG 787 CATATG PflMI 1003 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1093 CCAGCGCATGG PmaCI 2118 CACGTG CAC^GTG 2248 CACGTG PmlI 2118 CACGTG CAC^GTG 2248 CACGTG PvuII 2174 CAGCTG CAG^CTG 2422 CAGCTG SmlI 2056 CTCAAG C^T[CT][GA]AG 3032 CTCAAG Van91I 1003 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1093 CCAGCGCATGG XcmI 2014 CCAGGGAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2187 CCAAGTACATTCTGG XhoII 1200 GGATCC [GA]^GATC[CT] 1659 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflFI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I