################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII AccI AccII AccIII AclI AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AviII AvrII BamHI BanII BbrPI BbvCI BcgI BclI BglI BglII BlnI BsaAI BsaBI BsaI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoT22I FbaI FseI FspI HgaI HindIII HpaI KspI MamI MfeI MluI MroI MunI MvnI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 502 GGTACC G^GTACC AcyI 2993 GGCGCC G[GA]^CG[CT]C AflII 3277 CTTAAG C^TTAAG AgeI 2617 ACCGGT A^CCGGT AlwNI 2555 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 2783 ATTAAT AT^TAAT AsnI 2783 ATTAAT AT^TAAT Asp718I 502 GGTACC G^GTACC AvaI 3380 CCCGAG C^[CT]CG[GA]G BbeI 2993 GGCGCC GGCGC^C BbsI 146 GAAGAC GAAGAC(2/6) BciVI 2258 GTATCC GTATCC(6/5) BcnI 262 CCCGG CC^[GC]GG BfrI 3277 CTTAAG C^TTAAG BlpI 21 GCTGAGC GC^T[ACGT]AGC Bpu1102I 21 GCTGAGC GC^T[ACGT]AGC BpuAI 146 GAAGAC GAAGAC(2/6) BsaHI 2993 GGCGCC G[GA]^CG[CT]C BsmBI 1917 CGTCTC CGTCTC(1/5) BsoBI 3380 CCCGAG C^[CT]CG[GA]G BsrFI 2617 ACCGGT [GA]^CCGG[CT] BssSI 2906 CTCGTG CACGAG(-5/-1) CelII 21 GCTGAGC GC^T[ACGT]AGC Cfr10I 2617 ACCGGT [GA]^CCGG[CT] DsaI 3666 CCGTGG C^C[GA][CT]GG EcoRV 1134 GATATC GAT^ATC FauI 106 CCCGC CCCGC(4/6) Hin1I 2993 GGCGCC G[GA]^CG[CT]C HincII 1230 GTTGAC GT[CT]^[GA]AC HindII 1230 GTTGAC GT[CT]^[GA]AC KasI 2993 GGCGCC G^GCGCC KpnI 502 GGTACC GGTAC^C NarI 2993 GGCGCC GG^CGCC NciI 262 CCCGG CC^[GC]GG NdeI 3216 CATATG CA^TATG PflMI 2852 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2617 ACCGGT A^CCGGT PshAI 3830 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2783 ATTAAT AT^TAAT PstI 3551 CTGCAG CTGCA^G SfoI 2993 GGCGCC GGC^GCC StuI 1857 AGGCCT AGG^CCT Van91I 2852 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1502 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 107 CCGC CCGC(-3/-1) 2 GCGG BsmFI 2696 GGGAC GGGAC(10/14) 3791 GTCCC BsmI 3521 GAATGC GAATGC(1/-1) 3166 GCATTC BspHI 1976 TCATGA T^CATGA 3146 TCATGA BsrDI 1210 CATTGC GCAATG(2/0) 1523 CATTGC BstEII 1017 GGTGACC G^GT[ACGT]ACC 1302 GGTGACC BstPI 1017 GGTGACC G^GT[ACGT]ACC 1302 GGTGACC BstYI 1800 GGATCT [GA]^GATC[CT] 2734 AGATCC DraIII 526 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG 2112 CACTCAGTG DrdI 1888 GACTTTTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2616 GACCGGTTGGTC EcoNI 1812 CCTCAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2175 CCTCTGAGAGG EcoO65I 1017 GGTGACC G^GT[ACGT]ACC 1302 GGTGACC EcoRI 1053 GAATTC G^AATTC 2517 GAATTC MflI 1800 GGATCT [GA]^GATC[CT] 2734 AGATCC MspA1I 816 CAGCTG C[AC]G^C[GT]G 3161 CAGCTG PleI 411 GAGTC GAGTC(4/5) 2575 GAGTC PpuMI 3562 AGGACCC [GA]G^G[AT]CC[CT] 3789 GGGTCCC PvuII 816 CAGCTG CAG^CTG 3161 CAGCTG RcaI 1976 TCATGA T^CATGA 3146 TCATGA SfaNI 1470 GATGC GCATC(5/9) 2655 GATGC SmlI 1111 CTCAAG C^T[CT][GA]AG 3277 CTTAAG XcmI 1912 CCAAACGTCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2111 CCACTCAGTGAATGG XhoII 1800 GGATCT [GA]^GATC[CT] 2734 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AspHI AvaII BalI BanI BbvI BfaI BmyI BpmI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I