##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflIII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I AspEI
AspI AviII AvrII BamHI BanII
BbrPI BbvCI BcgI BclI BglI
BglII BlnI BsaAI BsaBI BsaI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoT22I FbaI FseI FspI HgaI
HindIII HpaI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 502 GGTACC G^GTACC
AcyI 2993 GGCGCC G[GA]^CG[CT]C
AflII 3277 CTTAAG C^TTAAG
AgeI 2617 ACCGGT A^CCGGT
AlwNI 2555 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 2783 ATTAAT AT^TAAT
AsnI 2783 ATTAAT AT^TAAT
Asp718I 502 GGTACC G^GTACC
AvaI 3380 CCCGAG C^[CT]CG[GA]G
BbeI 2993 GGCGCC GGCGC^C
BbsI 146 GAAGAC GAAGAC(2/6)
BciVI 2258 GTATCC GTATCC(6/5)
BcnI 262 CCCGG CC^[GC]GG
BfrI 3277 CTTAAG C^TTAAG
BlpI 21 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 21 GCTGAGC GC^T[ACGT]AGC
BpuAI 146 GAAGAC GAAGAC(2/6)
BsaHI 2993 GGCGCC G[GA]^CG[CT]C
BsmBI 1917 CGTCTC CGTCTC(1/5)
BsoBI 3380 CCCGAG C^[CT]CG[GA]G
BsrFI 2617 ACCGGT [GA]^CCGG[CT]
BssSI 2906 CTCGTG CACGAG(-5/-1)
CelII 21 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2617 ACCGGT [GA]^CCGG[CT]
DsaI 3666 CCGTGG C^C[GA][CT]GG
EcoRV 1134 GATATC GAT^ATC
FauI 106 CCCGC CCCGC(4/6)
Hin1I 2993 GGCGCC G[GA]^CG[CT]C
HincII 1230 GTTGAC GT[CT]^[GA]AC
HindII 1230 GTTGAC GT[CT]^[GA]AC
KasI 2993 GGCGCC G^GCGCC
KpnI 502 GGTACC GGTAC^C
NarI 2993 GGCGCC GG^CGCC
NciI 262 CCCGG CC^[GC]GG
NdeI 3216 CATATG CA^TATG
PflMI 2852 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2617 ACCGGT A^CCGGT
PshAI 3830 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2783 ATTAAT AT^TAAT
PstI 3551 CTGCAG CTGCA^G
SfoI 2993 GGCGCC GGC^GCC
StuI 1857 AGGCCT AGG^CCT
Van91I 2852 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1502 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 107 CCGC CCGC(-3/-1)
2 GCGG
BsmFI 2696 GGGAC GGGAC(10/14)
3791 GTCCC
BsmI 3521 GAATGC GAATGC(1/-1)
3166 GCATTC
BspHI 1976 TCATGA T^CATGA
3146 TCATGA
BsrDI 1210 CATTGC GCAATG(2/0)
1523 CATTGC
BstEII 1017 GGTGACC G^GT[ACGT]ACC
1302 GGTGACC
BstPI 1017 GGTGACC G^GT[ACGT]ACC
1302 GGTGACC
BstYI 1800 GGATCT [GA]^GATC[CT]
2734 AGATCC
DraIII 526 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
2112 CACTCAGTG
DrdI 1888 GACTTTTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2616 GACCGGTTGGTC
EcoNI 1812 CCTCAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2175 CCTCTGAGAGG
EcoO65I 1017 GGTGACC G^GT[ACGT]ACC
1302 GGTGACC
EcoRI 1053 GAATTC G^AATTC
2517 GAATTC
MflI 1800 GGATCT [GA]^GATC[CT]
2734 AGATCC
MspA1I 816 CAGCTG C[AC]G^C[GT]G
3161 CAGCTG
PleI 411 GAGTC GAGTC(4/5)
2575 GAGTC
PpuMI 3562 AGGACCC [GA]G^G[AT]CC[CT]
3789 GGGTCCC
PvuII 816 CAGCTG CAG^CTG
3161 CAGCTG
RcaI 1976 TCATGA T^CATGA
3146 TCATGA
SfaNI 1470 GATGC GCATC(5/9)
2655 GATGC
SmlI 1111 CTCAAG C^T[CT][GA]AG
3277 CTTAAG
XcmI 1912 CCAAACGTCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2111 CCACTCAGTGAATGG
XhoII 1800 GGATCT [GA]^GATC[CT]
2734 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BalI BanI BbvI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I