################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII AclI AcyI AflII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI AspEI BbeI BbrPI BcgI BciVI BclI BfrI BsaHI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstPI ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI Hin1I HindIII HpaI KasI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 3293 GTATAC GT^[AC][GT]AC AccIII 2996 TCCGGA T^CCGGA AcsI 1759 GAATTT [GA]^AATT[CT] AocI 3510 CCTGAGG CC^T[ACGT]AGG Aor51HI 1713 AGCGCT AGC^GCT ApoI 1759 GAATTT [GA]^AATT[CT] Asp700I 468 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 2483 GAGCTC G[AT]GC[AT]^C AviII 3318 TGCGCA TGC^GCA BamHI 166 GGATCC G^GATCC BglI 1499 GCCATCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 735 AGATCT A^GATCT BsaAI 3638 CACGTA [CT]AC^GT[GA] BsaWI 2996 TCCGGA [AT]^CCGG[AT] BseAI 2996 TCCGGA T^CCGGA BsiHKAI 2483 GAGCTC G[AT]GC[AT]^C BspEI 2996 TCCGGA T^CCGGA BspLU11I 1047 ACATGT A^CATGT BsrFI 2166 GCCGGC [GA]^CCGG[CT] Bst1107I 3293 GTATAC GTA^TAC BstZ17I 3293 GTATAC GTA^TAC Bsu36I 3510 CCTGAGG CC^T[ACGT]AGG Cfr10I 2166 GCCGGC [GA]^CCGG[CT] DraIII 2728 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 298 GACCTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1235 CTCTTC CTCTTC(1/4) Eco47III 1713 AGCGCT AGC^GCT Eco81I 3510 CCTGAGG CC^T[ACGT]AGG FspI 3318 TGCGCA TGC^GCA HincII 1181 GTCAAC GT[CT]^[GA]AC HindII 1181 GTCAAC GT[CT]^[GA]AC Ksp632I 1235 CTCTTC CTCTTC(1/4) KspI 659 CCGCGG CCGC^GG MroI 2996 TCCGGA T^CCGGA NaeI 2166 GCCGGC GCC^GGC NgoMIV 2166 GCCGGC G^CCGGC PflMI 1885 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 2483 GAGCTC GAGCT^C SacII 659 CCGCGG CCGC^GG ScaI 1989 AGTACT AGT^ACT SexAI 1703 ACCTGGT A^CC[AT]GGT TaqI 476 TCGA T^CGA TthHB8I 476 TCGA T^CGA Van91I 1885 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3547 TCTAGA T^CTAGA XmnI 468 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 1047 ACATGT A^C[GA][CT]GT 3880 ACGTGT AspI 564 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1155 GACAGTGTC AvrII 3030 CCTAGG C^CTAGG 3471 CCTAGG BalI 937 TGGCCA TGG^CCA 3169 TGGCCA BlnI 3030 CCTAGG C^CTAGG 3471 CCTAGG BlpI 905 GCTCAGC GC^T[ACGT]AGC 3270 GCTTAGC Bpu1102I 905 GCTCAGC GC^T[ACGT]AGC 3270 GCTTAGC BsaBI 1678 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2560 GATCAGCATC BsmI 2337 GAATGC GAATGC(1/-1) 2527 GAATGC BsrDI 512 CATTGC GCAATG(2/0) 1385 GCAATG BssSI 1472 CTCGTG CACGAG(-5/-1) 3304 CTCGTG CelII 905 GCTCAGC GC^T[ACGT]AGC 3270 GCTTAGC MamI 1678 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2560 GATCAGCATC MluNI 937 TGGCCA TGG^CCA 3169 TGGCCA MscI 937 TGGCCA TGG^CCA 3169 TGGCCA NcoI 940 CCATGG C^CATGG 3340 CCATGG PflFI 564 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1155 GACAGTGTC PstI 1 CTGCAG CTGCA^G 2681 CTGCAG SmlI 835 CTTGAG C^T[CT][GA]AG 3096 CTTGAG SphI 533 GCATGC GCATG^C 1074 GCATGC Tth111I 564 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1155 GACAGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### Acc65I AccII AciI AfaI AluI AlwI AlwNI Asp718I AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KpnI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmaCI XmaI