##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI AspEI BbeI BbrPI
BcgI BciVI BclI BfrI BsaHI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BsrBI BsrGI BssHII BstAPI
BstBI BstEII BstPI ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI Hin1I HindIII HpaI KasI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 3293 GTATAC GT^[AC][GT]AC
AccIII 2996 TCCGGA T^CCGGA
AcsI 1759 GAATTT [GA]^AATT[CT]
AocI 3510 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1713 AGCGCT AGC^GCT
ApoI 1759 GAATTT [GA]^AATT[CT]
Asp700I 468 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2483 GAGCTC G[AT]GC[AT]^C
AviII 3318 TGCGCA TGC^GCA
BamHI 166 GGATCC G^GATCC
BglI 1499 GCCATCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 735 AGATCT A^GATCT
BsaAI 3638 CACGTA [CT]AC^GT[GA]
BsaWI 2996 TCCGGA [AT]^CCGG[AT]
BseAI 2996 TCCGGA T^CCGGA
BsiHKAI 2483 GAGCTC G[AT]GC[AT]^C
BspEI 2996 TCCGGA T^CCGGA
BspLU11I 1047 ACATGT A^CATGT
BsrFI 2166 GCCGGC [GA]^CCGG[CT]
Bst1107I 3293 GTATAC GTA^TAC
BstZ17I 3293 GTATAC GTA^TAC
Bsu36I 3510 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2166 GCCGGC [GA]^CCGG[CT]
DraIII 2728 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 298 GACCTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1235 CTCTTC CTCTTC(1/4)
Eco47III 1713 AGCGCT AGC^GCT
Eco81I 3510 CCTGAGG CC^T[ACGT]AGG
FspI 3318 TGCGCA TGC^GCA
HincII 1181 GTCAAC GT[CT]^[GA]AC
HindII 1181 GTCAAC GT[CT]^[GA]AC
Ksp632I 1235 CTCTTC CTCTTC(1/4)
KspI 659 CCGCGG CCGC^GG
MroI 2996 TCCGGA T^CCGGA
NaeI 2166 GCCGGC GCC^GGC
NgoMIV 2166 GCCGGC G^CCGGC
PflMI 1885 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 2483 GAGCTC GAGCT^C
SacII 659 CCGCGG CCGC^GG
ScaI 1989 AGTACT AGT^ACT
SexAI 1703 ACCTGGT A^CC[AT]GGT
TaqI 476 TCGA T^CGA
TthHB8I 476 TCGA T^CGA
Van91I 1885 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3547 TCTAGA T^CTAGA
XmnI 468 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 1047 ACATGT A^C[GA][CT]GT
3880 ACGTGT
AspI 564 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1155 GACAGTGTC
AvrII 3030 CCTAGG C^CTAGG
3471 CCTAGG
BalI 937 TGGCCA TGG^CCA
3169 TGGCCA
BlnI 3030 CCTAGG C^CTAGG
3471 CCTAGG
BlpI 905 GCTCAGC GC^T[ACGT]AGC
3270 GCTTAGC
Bpu1102I 905 GCTCAGC GC^T[ACGT]AGC
3270 GCTTAGC
BsaBI 1678 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2560 GATCAGCATC
BsmI 2337 GAATGC GAATGC(1/-1)
2527 GAATGC
BsrDI 512 CATTGC GCAATG(2/0)
1385 GCAATG
BssSI 1472 CTCGTG CACGAG(-5/-1)
3304 CTCGTG
CelII 905 GCTCAGC GC^T[ACGT]AGC
3270 GCTTAGC
MamI 1678 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2560 GATCAGCATC
MluNI 937 TGGCCA TGG^CCA
3169 TGGCCA
MscI 937 TGGCCA TGG^CCA
3169 TGGCCA
NcoI 940 CCATGG C^CATGG
3340 CCATGG
PflFI 564 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1155 GACAGTGTC
PstI 1 CTGCAG CTGCA^G
2681 CTGCAG
SmlI 835 CTTGAG C^T[CT][GA]AG
3096 CTTGAG
SphI 533 GCATGC GCATG^C
1074 GCATGC
Tth111I 564 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1155 GACAGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
AlwI AlwNI Asp718I AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI KpnI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmaCI XmaI