##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
BamHI BbeI BcgI BcnI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrDI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KpnI MfeI MluI MroI MunI
NarI NciI NdeI NheI NotI
NruI NsiI PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 559 GAATTT [GA]^AATT[CT]
AcyI 1216 GACGCC G[GA]^CG[CT]C
Alw44I 626 GTGCAC G^TGCAC
AocI 3096 CCTGAGG CC^T[ACGT]AGG
ApaLI 626 GTGCAC G^TGCAC
ApoI 559 GAATTT [GA]^AATT[CT]
Asp700I 2422 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1951 CCTAGG C^CTAGG
BalI 1233 TGGCCA TGG^CCA
BbrPI 2639 CACGTG CAC^GTG
BciVI 425 GGATAC GTATCC(6/5)
BglII 2024 AGATCT A^GATCT
BlnI 1951 CCTAGG C^CTAGG
BlpI 662 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 662 GCTCAGC GC^T[ACGT]AGC
BsaBI 2854 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1216 GACGCC G[GA]^CG[CT]C
BsaWI 3410 ACCGGA [AT]^CCGG[AT]
BsiEI 1174 CGACCG CG[GA][CT]^CG
BssHII 196 GCGCGC G^CGCGC
BstAPI 44 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2651 CCATGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3096 CCTGAGG CC^T[ACGT]AGG
CelII 662 GCTCAGC GC^T[ACGT]AGC
DraI 2149 TTTAAA TTT^AAA
Eco81I 3096 CCTGAGG CC^T[ACGT]AGG
EcoNI 1138 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 71 AGCGCC [GA]GCGC^[CT]
Hin1I 1216 GACGCC G[GA]^CG[CT]C
KspI 58 CCGCGG CCGC^GG
MamI 2854 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1233 TGGCCA TGG^CCA
MscI 1233 TGGCCA TGG^CCA
PaeR7I 67 CTCGAG C^TCGAG
PmaCI 2639 CACGTG CAC^GTG
PmlI 2639 CACGTG CAC^GTG
SacII 58 CCGCGG CCGC^GG
SphI 3075 GCATGC GCATG^C
SspI 2889 AATATT AAT^ATT
StuI 1054 AGGCCT AGG^CCT
XcmI 357 CCAAGACCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 67 CTCGAG C^TCGAG
XmnI 2422 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwNI 1997 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3192 CAGAACCTG
ApaI 1134 GGGCCC GGGCC^C
1638 GGGCCC
BbsI 2197 GAAGAC GAAGAC(2/6)
2624 GTCTTC
BbvCI 149 GCTGAGG CCTCAGC(-5/-2)
235 GCTGAGG
BclI 1471 TGATCA T^GATCA
3304 TGATCA
BglI 1047 GCCGCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1631 GCCCACAGGGC
BpuAI 2197 GAAGAC GAAGAC(2/6)
2624 GTCTTC
BsaAI 773 TACGTG [CT]AC^GT[GA]
2639 CACGTG
BsgI 1894 CTGCAC GTGCAG(16/14)
776 GTGCAG
Bsp1407I 1092 TGTACA T^GTACA
3232 TGTACA
BsrBI 1048 CCGCTC CCGCTC(-3/-3)
2490 CCGCTC
BsrFI 212 GCCGGC [GA]^CCGG[CT]
635 GCCGGC
BsrGI 1092 TGTACA T^GTACA
3232 TGTACA
BstBI 380 TTCGAA TT^CGAA
3504 TTCGAA
Cfr10I 212 GCCGGC [GA]^CCGG[CT]
635 GCCGGC
FbaI 1471 TGATCA T^GATCA
3304 TGATCA
HincII 458 GTCAAC GT[CT]^[GA]AC
2221 GTCAAC
HindII 458 GTCAAC GT[CT]^[GA]AC
2221 GTCAAC
NaeI 212 GCCGGC GCC^GGC
635 GCCGGC
NcoI 75 CCATGG C^CATGG
2338 CCATGG
NgoMIV 212 GCCGGC G^CCGGC
635 GCCGGC
NspI 612 GCATGT [GA]CATG^[CT]
3075 GCATGC
NspV 380 TTCGAA TT^CGAA
3504 TTCGAA
PpuMI 878 GGGACCT [GA]G^G[AT]CC[CT]
1586 GGGTCCT
PstI 1444 CTGCAG CTGCA^G
2497 CTGCAG
PvuII 665 CAGCTG CAG^CTG
798 CAGCTG
SapI 2184 GAAGAGC GCTCTTC(1/4)
1033 GCTCTTC
SfiI 1046 GGCCGCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1630 GGCCCACAGGGCC
SfuI 380 TTCGAA TT^CGAA
3504 TTCGAA
SspBI 1092 TGTACA T^GTACA
3232 TGTACA
XbaI 245 TCTAGA T^CTAGA
1603 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AccII AciI AfaI AflII
AluI AlwI AspHI AvaI AvaII
BanI BanII BbvI BfaI BfrI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII