################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BbeI BcgI BcnI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI MfeI MluI MroI MunI NarI NciI NdeI NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 559 GAATTT [GA]^AATT[CT] AcyI 1216 GACGCC G[GA]^CG[CT]C Alw44I 626 GTGCAC G^TGCAC AocI 3096 CCTGAGG CC^T[ACGT]AGG ApaLI 626 GTGCAC G^TGCAC ApoI 559 GAATTT [GA]^AATT[CT] Asp700I 2422 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 1951 CCTAGG C^CTAGG BalI 1233 TGGCCA TGG^CCA BbrPI 2639 CACGTG CAC^GTG BciVI 425 GGATAC GTATCC(6/5) BglII 2024 AGATCT A^GATCT BlnI 1951 CCTAGG C^CTAGG BlpI 662 GCTCAGC GC^T[ACGT]AGC Bpu1102I 662 GCTCAGC GC^T[ACGT]AGC BsaBI 2854 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1216 GACGCC G[GA]^CG[CT]C BsaWI 3410 ACCGGA [AT]^CCGG[AT] BsiEI 1174 CGACCG CG[GA][CT]^CG BssHII 196 GCGCGC G^CGCGC BstAPI 44 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2651 CCATGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 3096 CCTGAGG CC^T[ACGT]AGG CelII 662 GCTCAGC GC^T[ACGT]AGC DraI 2149 TTTAAA TTT^AAA Eco81I 3096 CCTGAGG CC^T[ACGT]AGG EcoNI 1138 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 71 AGCGCC [GA]GCGC^[CT] Hin1I 1216 GACGCC G[GA]^CG[CT]C KspI 58 CCGCGG CCGC^GG MamI 2854 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1233 TGGCCA TGG^CCA MscI 1233 TGGCCA TGG^CCA PaeR7I 67 CTCGAG C^TCGAG PmaCI 2639 CACGTG CAC^GTG PmlI 2639 CACGTG CAC^GTG SacII 58 CCGCGG CCGC^GG SphI 3075 GCATGC GCATG^C SspI 2889 AATATT AAT^ATT StuI 1054 AGGCCT AGG^CCT XcmI 357 CCAAGACCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 67 CTCGAG C^TCGAG XmnI 2422 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AlwNI 1997 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG 3192 CAGAACCTG ApaI 1134 GGGCCC GGGCC^C 1638 GGGCCC BbsI 2197 GAAGAC GAAGAC(2/6) 2624 GTCTTC BbvCI 149 GCTGAGG CCTCAGC(-5/-2) 235 GCTGAGG BclI 1471 TGATCA T^GATCA 3304 TGATCA BglI 1047 GCCGCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1631 GCCCACAGGGC BpuAI 2197 GAAGAC GAAGAC(2/6) 2624 GTCTTC BsaAI 773 TACGTG [CT]AC^GT[GA] 2639 CACGTG BsgI 1894 CTGCAC GTGCAG(16/14) 776 GTGCAG Bsp1407I 1092 TGTACA T^GTACA 3232 TGTACA BsrBI 1048 CCGCTC CCGCTC(-3/-3) 2490 CCGCTC BsrFI 212 GCCGGC [GA]^CCGG[CT] 635 GCCGGC BsrGI 1092 TGTACA T^GTACA 3232 TGTACA BstBI 380 TTCGAA TT^CGAA 3504 TTCGAA Cfr10I 212 GCCGGC [GA]^CCGG[CT] 635 GCCGGC FbaI 1471 TGATCA T^GATCA 3304 TGATCA HincII 458 GTCAAC GT[CT]^[GA]AC 2221 GTCAAC HindII 458 GTCAAC GT[CT]^[GA]AC 2221 GTCAAC NaeI 212 GCCGGC GCC^GGC 635 GCCGGC NcoI 75 CCATGG C^CATGG 2338 CCATGG NgoMIV 212 GCCGGC G^CCGGC 635 GCCGGC NspI 612 GCATGT [GA]CATG^[CT] 3075 GCATGC NspV 380 TTCGAA TT^CGAA 3504 TTCGAA PpuMI 878 GGGACCT [GA]G^G[AT]CC[CT] 1586 GGGTCCT PstI 1444 CTGCAG CTGCA^G 2497 CTGCAG PvuII 665 CAGCTG CAG^CTG 798 CAGCTG SapI 2184 GAAGAGC GCTCTTC(1/4) 1033 GCTCTTC SfiI 1046 GGCCGCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1630 GGCCCACAGGGCC SfuI 380 TTCGAA TT^CGAA 3504 TTCGAA SspBI 1092 TGTACA T^GTACA 3232 TGTACA XbaI 245 TCTAGA T^CTAGA 1603 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccI AccII AciI AfaI AflII AluI AlwI AspHI AvaI AvaII BanI BanII BbvI BfaI BfrI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII