##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AvaI AviII
AvrII BamHI BanI BbeI BbvCI
BcgI BcnI BglI BlnI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BspMI BsrBI BssHII BstAPI BstBI
BstEII BstPI BstUI ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 2924 CTTAAG C^TTAAG
Alw44I 2865 GTGCAC G^TGCAC
AlwI 420 GATCC GGATC(4/5)
AocI 1339 CCTAAGG CC^T[ACGT]AGG
ApaLI 2865 GTGCAC G^TGCAC
AvaII 2355 GGTCC G^G[AT]CC
BalI 526 TGGCCA TGG^CCA
BbrPI 32 CACGTG CAC^GTG
BfrI 2924 CTTAAG C^TTAAG
BglII 2807 AGATCT A^GATCT
BsaWI 807 ACCGGA [AT]^CCGG[AT]
BseRI 970 GAGGAG GAGGAG(10/8)
BsmI 739 GCATTC GAATGC(1/-1)
Bsp1407I 3078 TGTACA T^GTACA
BsrFI 54 GCCGGT [GA]^CCGG[CT]
BsrGI 3078 TGTACA T^GTACA
Bst1107I 1962 GTATAC GTA^TAC
BstZ17I 1962 GTATAC GTA^TAC
Bsu36I 1339 CCTAAGG CC^T[ACGT]AGG
Cac8I 528 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 1886 GCGC GCG^C
Cfr10I 54 GCCGGT [GA]^CCGG[CT]
DsaI 2891 CCATGG C^C[GA][CT]GG
EaeI 526 TGGCCA [CT]^GGCC[GA]
Eco81I 1339 CCTAAGG CC^T[ACGT]AGG
EcoRI 2132 GAATTC G^AATTC
EcoRV 250 GATATC GAT^ATC
EcoT22I 737 ATGCAT ATGCA^T
FauI 532 GCGGG CCCGC(4/6)
HhaI 1886 GCGC GCG^C
HinP1I 1886 GCGC G^CGC
HindIII 3101 AAGCTT A^AGCTT
MluNI 526 TGGCCA TGG^CCA
MscI 526 TGGCCA TGG^CCA
MspA1I 530 CAGCGG C[AC]G^C[GT]G
NcoI 2891 CCATGG C^CATGG
NsiI 737 ATGCAT ATGCA^T
PmaCI 32 CACGTG CAC^GTG
PmlI 32 CACGTG CAC^GTG
PstI 1616 CTGCAG CTGCA^G
SspBI 3078 TGTACA T^GTACA
SspI 1519 AATATT AAT^ATT
TaqI 691 TCGA T^CGA
TthHB8I 691 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 834 GTAGAC GT^[AC][GT]AC
1962 GTATAC
AciI 532 GCGG CCGC(-3/-1)
2315 GCGG
AlwNI 418 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1221 CAGTACCTG
AseI 2476 ATTAAT AT^TAAT
2643 ATTAAT
AsnI 2476 ATTAAT AT^TAAT
2643 ATTAAT
Asp700I 152 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1505 GAAACATTTC
AspHI 2535 GTGCTC G[AT]GC[AT]^C
2865 GTGCAC
AspI 459 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2241 GACTGTGTC
BanII 1345 GAGCCC G[GA]GC[CT]^C
2398 GAGCCC
BciVI 774 GGATAC GTATCC(6/5)
2929 GGATAC
BclI 517 TGATCA T^GATCA
1132 TGATCA
BpmI 898 CTCCAG CTGGAG(16/14)
1175 CTGGAG
BsaAI 32 CACGTG [CT]AC^GT[GA]
3082 CACGTA
BsiHKAI 2535 GTGCTC G[AT]GC[AT]^C
2865 GTGCAC
BsiYI 461 CCAAGTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2263 CCACTTGTAGG
BslI 461 CCAAGTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2263 CCACTTGTAGG
BspLU11I 154 ACATGT A^CATGT
1557 ACATGT
BssSI 828 CTCGTG CACGAG(-5/-1)
2757 CTCGTG
BstXI 1199 CCAAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2650 CCAATAGTTTGG
BstYI 419 AGATCC [GA]^GATC[CT]
2807 AGATCT
Cfr13I 1171 GGCCC G^G[ACGT]CC
2355 GGTCC
FbaI 517 TGATCA T^GATCA
1132 TGATCA
HapII 55 CCGG C^CGG
808 CCGG
HpaII 55 CCGG C^CGG
808 CCGG
MflI 419 AGATCC [GA]^GATC[CT]
2807 AGATCT
MspI 55 CCGG C^CGG
808 CCGG
NlaIV 1177 GGAGCC GG[ACGT]^[ACGT]CC
1344 GGAGCC
NspI 154 ACATGT [GA]CATG^[CT]
1557 ACATGT
PflFI 459 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2241 GACTGTGTC
PshBI 2476 ATTAAT AT^TAAT
2643 ATTAAT
Sau96I 1171 GGCCC G^G[ACGT]CC
2355 GGTCC
SwaI 1402 ATTTAAAT ATTT^AAAT
1590 ATTTAAAT
Tth111I 459 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2241 GACTGTGTC
XhoII 419 AGATCC [GA]^GATC[CT]
2807 AGATCT
XmnI 152 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1505 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
BbsI BbvI BfaI BlpI BmyI
Bpu1102I BpuAI BsaJI BsgI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI CelII DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII PleI RsaI
Sau3AI ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI