##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI ApaI AscI AseI
AsnI Asp700I AspI AviII AvrII
BalI BbeI BbvCI BfrI BlnI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BsrDI
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I EcoRV EcoT22I FseI
FspI Hin1I KasI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3829 GGTACC G^GTACC
AflIII 1304 ACACGT A^C[GA][CT]GT
AhdI 1303 GACACGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3465 AGCGCT AGC^GCT
Asp718I 3829 GGTACC G^GTACC
AspEI 1303 GACACGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1871 CCCGAG C^[CT]CG[GA]G
BbrPI 1440 CACGTG CAC^GTG
BcgI 2523 CGATGGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1314 TGATCA T^GATCA
BglI 3121 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 327 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 327 GCTGAGC GC^T[ACGT]AGC
BsaAI 1440 CACGTG [CT]AC^GT[GA]
BsmBI 1758 GAGACG CGTCTC(1/5)
BsmI 3165 GCATTC GAATGC(1/-1)
BsoBI 1871 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1670 TGTACA T^GTACA
BspHI 419 TCATGA T^CATGA
BsrGI 1670 TGTACA T^GTACA
BstEII 1703 GGTGACC G^GT[ACGT]ACC
BstPI 1703 GGTGACC G^GT[ACGT]ACC
BstXI 1104 CCACAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 327 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1303 GACACGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3465 AGCGCT AGC^GCT
EcoO65I 1703 GGTGACC G^GT[ACGT]ACC
EcoRI 3948 GAATTC G^AATTC
FbaI 1314 TGATCA T^GATCA
HgaI 934 GCGTC GACGC(5/10)
HindIII 2324 AAGCTT A^AGCTT
KpnI 3829 GGTACC GGTAC^C
NcoI 3351 CCATGG C^CATGG
NdeI 94 CATATG CA^TATG
NheI 216 GCTAGC G^CTAGC
PmaCI 1440 CACGTG CAC^GTG
PmlI 1440 CACGTG CAC^GTG
PpuMI 2344 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1680 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 419 TCATGA T^CATGA
SbfI 1552 CCTGCAGG CCTGCA^GG
Sse8387I 1552 CCTGCAGG CCTGCA^GG
SspBI 1670 TGTACA T^GTACA
SspI 3740 AATATT AAT^ATT
SwaI 2438 ATTTAAAT ATTT^AAAT
TaqI 685 TCGA T^CGA
TthHB8I 685 TCGA T^CGA
XbaI 3782 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 1479 GTGCAC G^TGCAC
2235 GTGCAC
AlwNI 1447 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
3406 CAGTCACTG
ApaLI 1479 GTGCAC G^TGCAC
2235 GTGCAC
BciVI 1765 GGATAC GTATCC(6/5)
2697 GTATCC
BcnI 51 CCGGG CC^[GC]GG
709 CCGGG
BglII 1930 AGATCT A^GATCT
3517 AGATCT
BsrBI 65 GAGCGG CCGCTC(-3/-3)
2539 GAGCGG
BsrFI 22 ACCGGC [GA]^CCGG[CT]
2656 GCCGGT
BssSI 2357 CTCGTG CACGAG(-5/-1)
3058 CTCGTG
Cfr10I 22 ACCGGC [GA]^CCGG[CT]
2656 GCCGGT
DraII 647 GGGGCCT [GA]G^G[ACGT]CC[CT]
2344 GGGTCCT
DsaI 2534 CCGTGG C^C[GA][CT]GG
3351 CCATGG
EaeI 125 CGGCCA [CT]^GGCC[GA]
747 TGGCCG
EcoNI 2593 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3676 CCTCCCCAAGG
EcoO109I 647 GGGGCCT [GA]G^G[ACGT]CC[CT]
2344 GGGTCCT
HincII 1359 GTTAAC GT[CT]^[GA]AC
2977 GTTAAC
HindII 1359 GTTAAC GT[CT]^[GA]AC
2977 GTTAAC
HpaI 1359 GTTAAC GTT^AAC
2977 GTTAAC
NciI 51 CCGGG CC^[GC]GG
709 CCGGG
PflMI 1446 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3557 CCAGCACTTGG
SapI 423 GAAGAGC GCTCTTC(1/4)
2617 GAAGAGC
SphI 32 GCATGC GCATG^C
2511 GCATGC
StuI 1784 AGGCCT AGG^CCT
3627 AGGCCT
Van91I 1446 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3557 CCAGCACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaII
BamHI BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI Eco81I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII