##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AflII Aor51HI ApaI AscI
Asp718I AspI AviII BamHI BbeI
BbrPI BciVI BfrI BsaAI BsaBI
BsiWI BsmBI BsmI BspDI BspHI
BssSI BstAPI BstBI ClaI CpoI
Eco47III EcoRI EcoRV EcoT22I FspI
HaeII HpaI KasI KpnI KspI
MamI MluI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 2730 GACGTC GACGT^C
AccIII 2309 TCCGGA T^CCGGA
AcsI 2463 GAATTT [GA]^AATT[CT]
AgeI 1125 ACCGGT A^CCGGT
AhdI 17 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3154 GTGCAC G^TGCAC
AocI 1613 CCTCAGG CC^T[ACGT]AGG
ApaLI 3154 GTGCAC G^TGCAC
ApoI 2463 GAATTT [GA]^AATT[CT]
AseI 76 ATTAAT AT^TAAT
AsnI 76 ATTAAT AT^TAAT
AspEI 17 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1888 CCCGAG C^[CT]CG[GA]G
AvrII 2964 CCTAGG C^CTAGG
BglII 3209 AGATCT A^GATCT
BlnI 2964 CCTAGG C^CTAGG
BseAI 2309 TCCGGA T^CCGGA
BsgI 3302 CTGCAC GTGCAG(16/14)
BsoBI 1888 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1549 TGTACA T^GTACA
BspEI 2309 TCCGGA T^CCGGA
BspMI 1572 ACCTGC ACCTGC(4/8)
BsrBI 959 CCGCTC CCGCTC(-3/-3)
BsrGI 1549 TGTACA T^GTACA
BssHII 3112 GCGCGC G^CGCGC
Bst1107I 1220 GTATAC GTA^TAC
BstEII 847 GGTGACC G^GT[ACGT]ACC
BstPI 847 GGTGACC G^GT[ACGT]ACC
BstZ17I 1220 GTATAC GTA^TAC
Bsu36I 1613 CCTCAGG CC^T[ACGT]AGG
DraIII 131 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1204 CGGCCG C^GGCCG
Eam1105I 17 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1204 CGGCCG C^GGCCG
Eco52I 1204 CGGCCG C^GGCCG
Eco81I 1613 CCTCAGG CC^T[ACGT]AGG
EcoNI 2327 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 847 GGTGACC G^GT[ACGT]ACC
FseI 2146 GGCCGGCC GGCCGG^CC
HincII 802 GTCAAC GT[CT]^[GA]AC
HindII 802 GTCAAC GT[CT]^[GA]AC
MfeI 1545 CAATTG C^AATTG
MroI 2309 TCCGGA T^CCGGA
MunI 1545 CAATTG C^AATTG
NaeI 2147 GCCGGC GCC^GGC
NcoI 1431 CCATGG C^CATGG
NgoMIV 2147 GCCGGC G^CCGGC
NheI 971 GCTAGC G^CTAGC
PinAI 1125 ACCGGT A^CCGGT
PshAI 1754 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 76 ATTAAT AT^TAAT
PstI 2139 CTGCAG CTGCA^G
SspBI 1549 TGTACA T^GTACA
StuI 1078 AGGCCT AGG^CCT
XbaI 103 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1055 GTCTAC GT^[AC][GT]AC
1220 GTATAC
AclI 1708 AACGTT AA^CGTT
3390 AACGTT
AcyI 2051 GACGCC G[GA]^CG[CT]C
2730 GACGTC
AflIII 370 ACATGT A^C[GA][CT]GT
1743 ACATGT
AlwNI 2237 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2332 CAGAGGCTG
Asp700I 1102 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1562 GAACAGGTTC
BcgI 1159 CGAATCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2193 CGAGGGAGGTGC
BclI 480 TGATCA T^GATCA
1492 TGATCA
BglI 1974 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2233 GCCCCAGTGGC
BsaHI 2051 GACGCC G[GA]^CG[CT]C
2730 GACGTC
BsaWI 1125 ACCGGT [AT]^CCGG[AT]
2309 TCCGGA
BsiEI 976 CGACCG CG[GA][CT]^CG
1204 CGGCCG
BspLU11I 370 ACATGT A^CATGT
1743 ACATGT
BsrDI 916 CATTGC GCAATG(2/0)
2579 CATTGC
BstXI 754 CCAGATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1510 CCAGTTAGCTGG
DraI 592 TTTAAA TTT^AAA
3189 TTTAAA
DrdI 176 GACCCTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
793 GACAAGCGGGTC
DsaI 1431 CCATGG C^C[GA][CT]GG
1756 CCGTGG
FbaI 480 TGATCA T^GATCA
1492 TGATCA
Hin1I 2051 GACGCC G[GA]^CG[CT]C
2730 GACGTC
HindIII 1018 AAGCTT A^AGCTT
1394 AAGCTT
NspI 370 ACATGT [GA]CATG^[CT]
1743 ACATGT
PflMI 1808 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2331 CCAGAGGCTGG
Psp1406I 1708 AACGTT AA^CGTT
3390 AACGTT
SfcI 995 CTACAG C^T[GA][CT]AG
2139 CTGCAG
Van91I 1808 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2331 CCAGAGGCTGG
XmnI 1102 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1562 GAACAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII