##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII BbeI BbrPI BbvCI BclI
BfrI BglII BsaAI BsaBI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspI SwaI TaqI TthHB8I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 2807 GACGTC GACGT^C
AflIII 1963 ACACGT A^C[GA][CT]GT
AhdI 2525 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2525 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1731 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 479 CCTAGG C^CTAGG
BcgI 1652 GCAGGTGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2059 GTATCC GTATCC(6/5)
BlnI 479 CCTAGG C^CTAGG
BsaWI 2310 TCCGGT [AT]^CCGG[AT]
BsmBI 2809 CGTCTC CGTCTC(1/5)
Bsp1407I 350 TGTACA T^GTACA
BsrFI 2413 GCCGGC [GA]^CCGG[CT]
BsrGI 350 TGTACA T^GTACA
Cfr10I 2413 GCCGGC [GA]^CCGG[CT]
DraIII 1222 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2242 GACAGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2525 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 797 CCTTGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1533 GAATTC G^AATTC
HincII 2804 GTTGAC GT[CT]^[GA]AC
HindII 2804 GTTGAC GT[CT]^[GA]AC
HindIII 28 AAGCTT A^AGCTT
NaeI 2413 GCCGGC GCC^GGC
NgoMIV 2413 GCCGGC G^CCGGC
NspI 1060 GCATGC [GA]CATG^[CT]
PflFI 1731 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 93 CCTGCAGG CCTGCA^GG
ScaI 2683 AGTACT AGT^ACT
SphI 1060 GCATGC GCATG^C
Sse8387I 93 CCTGCAGG CCTGCA^GG
SspBI 350 TGTACA T^GTACA
Tth111I 1731 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2300 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 1320 CGCG CG^CG
1734 CGCG
AcsI 1215 AAATTT [GA]^AATT[CT]
1533 GAATTC
AfaI 351 GTAC GT^AC
2684 GTAC
ApaI 1588 GGGCCC GGGCC^C
2045 GGGCCC
ApoI 1215 AAATTT [GA]^AATT[CT]
1533 GAATTC
BamHI 692 GGATCC G^GATCC
1483 GGATCC
BbsI 2083 GAAGAC GAAGAC(2/6)
2261 GAAGAC
BglI 1236 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1581 GCCAAGCGGGC
BlpI 67 GCTGAGC GC^T[ACGT]AGC
1820 GCTCAGC
Bpu1102I 67 GCTGAGC GC^T[ACGT]AGC
1820 GCTCAGC
BpuAI 2083 GAAGAC GAAGAC(2/6)
2261 GAAGAC
BsaI 553 GAGACC GGTCTC(1/5)
2011 GAGACC
BspMI 3086 ACCTGC ACCTGC(4/8)
1652 GCAGGT
BstAPI 167 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1288 GCAGCTCTTGC
BstEII 2287 GGTCACC G^GT[ACGT]ACC
2817 GGTCACC
BstPI 2287 GGTCACC G^GT[ACGT]ACC
2817 GGTCACC
BstUI 1320 CGCG CG^CG
1734 CGCG
CelII 67 GCTGAGC GC^T[ACGT]AGC
1820 GCTCAGC
DsaI 671 CCATGG C^C[GA][CT]GG
2028 CCATGG
EcoO65I 2287 GGTCACC G^GT[ACGT]ACC
2817 GGTCACC
HaeII 121 AGCGCC [GA]GCGC^[CT]
1569 AGCGCC
MseI 1843 TTAA T^TAA
3123 TTAA
MvnI 1320 CGCG CG^CG
1734 CGCG
NcoI 671 CCATGG C^CATGG
2028 CCATGG
PpuMI 1192 GGGACCC [GA]G^G[AT]CC[CT]
2950 AGGACCT
RsaI 351 GTAC GT^AC
2684 GTAC
SacI 1160 GAGCTC GAGCT^C
1818 GAGCTC
SmaI 5 CCCGGG CCC^GGG
2999 CCCGGG
StuI 728 AGGCCT AGG^CCT
1686 AGGCCT
Tru9I 1843 TTAA T^TAA
3123 TTAA
XmaCI 5 CCCGGG C^CCGGG
2999 CCCGGG
XmaI 5 CCCGGG C^CCGGG
2999 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII