##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
AseI AsnI Asp718I BamHI BcgI
BciVI BclI BsiWI BspHI BspLU11I
BsrBI BssHII BstBI BstEII BstPI
CpoI DraI EcoO65I EcoRI EcoRV
FbaI FseI HpaI KpnI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SbfI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcyI 830 GGCGCC G[GA]^CG[CT]C
AflII 4179 CTTAAG C^TTAAG
AhdI 3732 GACTAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3890 GTGCAC G^TGCAC
Aor51HI 25 AGCGCT AGC^GCT
ApaLI 3890 GTGCAC G^TGCAC
Asp700I 236 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3732 GACTAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4171 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2043 CCTAGG C^CTAGG
BbeI 830 GGCGCC GGCGC^C
BfrI 4179 CTTAAG C^TTAAG
BlnI 2043 CCTAGG C^CTAGG
BlpI 3517 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3517 GCTGAGC GC^T[ACGT]AGC
BsaBI 1229 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 830 GGCGCC G[GA]^CG[CT]C
BsiEI 702 CGGCCG CG[GA][CT]^CG
BsmBI 3910 CGTCTC CGTCTC(1/5)
BsmI 4186 GCATTC GAATGC(1/-1)
BspDI 1496 ATCGAT AT^CGAT
BsrFI 3260 GCCGGT [GA]^CCGG[CT]
Bst1107I 1555 GTATAC GTA^TAC
BstAPI 2775 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1555 GTATAC GTA^TAC
CelII 3517 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3260 GCCGGT [GA]^CCGG[CT]
ClaI 1496 ATCGAT AT^CGAT
DrdI 742 GACCGAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 702 CGGCCG C^GGCCG
Eam1105I 3732 GACTAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 702 CGGCCG C^GGCCG
Eco47III 25 AGCGCT AGC^GCT
Eco52I 702 CGGCCG C^GGCCG
EcoT22I 3804 ATGCAT ATGCA^T
HgaI 2807 GCGTC GACGC(5/10)
Hin1I 830 GGCGCC G[GA]^CG[CT]C
HincII 733 GTCGAC GT[CT]^[GA]AC
HindII 733 GTCGAC GT[CT]^[GA]AC
KasI 830 GGCGCC G^GCGCC
KspI 1266 CCGCGG CCGC^GG
MamI 1229 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 830 GGCGCC GG^CGCC
NsiI 3804 ATGCAT ATGCA^T
PaeR7I 4026 CTCGAG C^TCGAG
PflFI 4171 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 1266 CCGCGG CCGC^GG
SalI 733 GTCGAC G^TCGAC
SfoI 830 GGCGCC GGC^GCC
Tth111I 4171 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4026 CTCGAG C^TCGAG
XmnI 236 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 309 TCCGGA T^CCGGA
423 TCCGGA
AcsI 1650 AAATTT [GA]^AATT[CT]
1673 AAATTC
AflIII 153 ACGTGT A^C[GA][CT]GT
1781 ACGTGT
ApoI 1650 AAATTT [GA]^AATT[CT]
1673 AAATTC
AviII 438 TGCGCA TGC^GCA
3608 TGCGCA
BbrPI 1780 CACGTG CAC^GTG
2193 CACGTG
BbsI 1057 GTCTTC GAAGAC(2/6)
1710 GTCTTC
BbvCI 2529 CCTCAGC CCTCAGC(-5/-2)
533 GCTGAGG
BglII 213 AGATCT A^GATCT
1369 AGATCT
BpuAI 1057 GTCTTC GAAGAC(2/6)
1710 GTCTTC
BsaI 3248 GAGACC GGTCTC(1/5)
1999 GGTCTC
BseAI 309 TCCGGA T^CCGGA
423 TCCGGA
BspEI 309 TCCGGA T^CCGGA
423 TCCGGA
BsrDI 1728 CATTGC GCAATG(2/0)
4061 GCAATG
BstXI 1512 CCAAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2822 CCAGCGACCTGG
FspI 438 TGCGCA TGC^GCA
3608 TGCGCA
HindIII 1142 AAGCTT A^AGCTT
4158 AAGCTT
MroI 309 TCCGGA T^CCGGA
423 TCCGGA
PmaCI 1780 CACGTG CAC^GTG
2193 CACGTG
PmlI 1780 CACGTG CAC^GTG
2193 CACGTG
PstI 757 CTGCAG CTGCA^G
3030 CTGCAG
SacI 385 GAGCTC GAGCT^C
3643 GAGCTC
SapI 2712 GAAGAGC GCTCTTC(1/4)
2085 GCTCTTC
ScaI 360 AGTACT AGT^ACT
1757 AGTACT
SfiI 628 GGCCCGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3432 GGCCTGCCTGGCC
SphI 3605 GCATGC GCATG^C
3806 GCATGC
StuI 414 AGGCCT AGG^CCT
1333 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI