##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Aor51HI AscI AspEI AspI
AviII BbrPI BciVI BclI BfrI
BsaAI BsaBI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI DrdI
Eam1105I Eco47III FbaI FseI FspI
HindIII KspI MamI MfeI MluI
MunI NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SgrAI SnaBI SpeI
SspBI SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 3596 GGTACC G^GTACC
AccI 968 GTAGAC GT^[AC][GT]AC
Alw44I 2376 GTGCAC G^TGCAC
ApaLI 2376 GTGCAC G^TGCAC
AseI 242 ATTAAT AT^TAAT
AsnI 242 ATTAAT AT^TAAT
Asp700I 2402 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3596 GGTACC G^GTACC
AvrII 1339 CCTAGG C^CTAGG
BglII 3832 AGATCT A^GATCT
BlnI 1339 CCTAGG C^CTAGG
BsmI 3759 GAATGC GAATGC(1/-1)
BssSI 1581 CTCGTG CACGAG(-5/-1)
BstBI 2782 TTCGAA TT^CGAA
BstEII 1877 GGTCACC G^GT[ACGT]ACC
BstPI 1877 GGTCACC G^GT[ACGT]ACC
DraI 4325 TTTAAA TTT^AAA
EcoO65I 1877 GGTCACC G^GT[ACGT]ACC
EcoRV 2480 GATATC GAT^ATC
EcoT22I 3494 ATGCAT ATGCA^T
HpaI 1646 GTTAAC GTT^AAC
KpnI 3596 GGTACC GGTAC^C
NaeI 263 GCCGGC GCC^GGC
NdeI 1842 CATATG CA^TATG
NgoMIV 263 GCCGGC G^CCGGC
NsiI 3494 ATGCAT ATGCA^T
NspV 2782 TTCGAA TT^CGAA
PshAI 3725 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 242 ATTAAT AT^TAAT
SexAI 2112 ACCAGGT A^CC[AT]GGT
SfiI 3535 GGCCGAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2782 TTCGAA TT^CGAA
SphI 896 GCATGC GCATG^C
TfiI 1447 GAATC G^A[AT]TC
XmnI 2402 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 2954 TCCGGA T^CCGGA
3206 TCCGGA
AcyI 344 GGCGCC G[GA]^CG[CT]C
1263 GGCGCC
AocI 807 CCTGAGG CC^T[ACGT]AGG
2977 CCTGAGG
BalI 1101 TGGCCA TGG^CCA
2789 TGGCCA
BamHI 1386 GGATCC G^GATCC
1519 GGATCC
BbeI 344 GGCGCC GGCGC^C
1263 GGCGCC
BbvCI 4161 CCTCAGC CCTCAGC(-5/-2)
3339 GCTGAGG
BcgI 2766 GCAACAGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3171 GCAGCTCAATCG
BlpI 2994 GCTAAGC GC^T[ACGT]AGC
4131 GCTCAGC
Bpu1102I 2994 GCTAAGC GC^T[ACGT]AGC
4131 GCTCAGC
BsaHI 344 GGCGCC G[GA]^CG[CT]C
1263 GGCGCC
BsaI 1772 GAGACC GGTCTC(1/5)
2698 GAGACC
BseAI 2954 TCCGGA T^CCGGA
3206 TCCGGA
BspEI 2954 TCCGGA T^CCGGA
3206 TCCGGA
BstAPI 1865 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3402 GCAACTGGTGC
BstXI 2702 CCAAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3470 CCAGTGTTCTGG
Bsu36I 807 CCTGAGG CC^T[ACGT]AGG
2977 CCTGAGG
CelII 2994 GCTAAGC GC^T[ACGT]AGC
4131 GCTCAGC
EagI 261 CGGCCG C^GGCCG
3437 CGGCCG
EclXI 261 CGGCCG C^GGCCG
3437 CGGCCG
Eco52I 261 CGGCCG C^GGCCG
3437 CGGCCG
Eco81I 807 CCTGAGG CC^T[ACGT]AGG
2977 CCTGAGG
EcoNI 589 CCTATGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1268 CCTGTGTCAGG
EcoRI 1922 GAATTC G^AATTC
2187 GAATTC
Hin1I 344 GGCGCC G[GA]^CG[CT]C
1263 GGCGCC
HincII 1176 GTTGAC GT[CT]^[GA]AC
1646 GTTAAC
HindII 1176 GTTGAC GT[CT]^[GA]AC
1646 GTTAAC
KasI 344 GGCGCC G^GCGCC
1263 GGCGCC
MaeII 233 ACGT A^CGT
4349 ACGT
MluNI 1101 TGGCCA TGG^CCA
2789 TGGCCA
MroI 2954 TCCGGA T^CCGGA
3206 TCCGGA
MscI 1101 TGGCCA TGG^CCA
2789 TGGCCA
NarI 344 GGCGCC GG^CGCC
1263 GGCGCC
NspI 896 GCATGC [GA]CATG^[CT]
3492 ACATGC
PaeR7I 1614 CTCGAG C^TCGAG
3485 CTCGAG
SbfI 1715 CCTGCAGG CCTGCA^GG
3117 CCTGCAGG
SfoI 344 GGCGCC GGC^GCC
1263 GGCGCC
Sse8387I 1715 CCTGCAGG CCTGCA^GG
3117 CCTGCAGG
XhoI 1614 CTCGAG C^TCGAG
3485 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NheI NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII XmaCI XmaI