################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AclI AflII AflIII AgeI AhdI Aor51HI AscI AspEI AspI AviII BbrPI BciVI BclI BfrI BsaAI BsaBI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstZ17I ClaI CpoI DrdI Eam1105I Eco47III FbaI FseI FspI HindIII KspI MamI MfeI MluI MunI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 3596 GGTACC G^GTACC AccI 968 GTAGAC GT^[AC][GT]AC Alw44I 2376 GTGCAC G^TGCAC ApaLI 2376 GTGCAC G^TGCAC AseI 242 ATTAAT AT^TAAT AsnI 242 ATTAAT AT^TAAT Asp700I 2402 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3596 GGTACC G^GTACC AvrII 1339 CCTAGG C^CTAGG BglII 3832 AGATCT A^GATCT BlnI 1339 CCTAGG C^CTAGG BsmI 3759 GAATGC GAATGC(1/-1) BssSI 1581 CTCGTG CACGAG(-5/-1) BstBI 2782 TTCGAA TT^CGAA BstEII 1877 GGTCACC G^GT[ACGT]ACC BstPI 1877 GGTCACC G^GT[ACGT]ACC DraI 4325 TTTAAA TTT^AAA EcoO65I 1877 GGTCACC G^GT[ACGT]ACC EcoRV 2480 GATATC GAT^ATC EcoT22I 3494 ATGCAT ATGCA^T HpaI 1646 GTTAAC GTT^AAC KpnI 3596 GGTACC GGTAC^C NaeI 263 GCCGGC GCC^GGC NdeI 1842 CATATG CA^TATG NgoMIV 263 GCCGGC G^CCGGC NsiI 3494 ATGCAT ATGCA^T NspV 2782 TTCGAA TT^CGAA PshAI 3725 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 242 ATTAAT AT^TAAT SexAI 2112 ACCAGGT A^CC[AT]GGT SfiI 3535 GGCCGAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 2782 TTCGAA TT^CGAA SphI 896 GCATGC GCATG^C TfiI 1447 GAATC G^A[AT]TC XmnI 2402 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccIII 2954 TCCGGA T^CCGGA 3206 TCCGGA AcyI 344 GGCGCC G[GA]^CG[CT]C 1263 GGCGCC AocI 807 CCTGAGG CC^T[ACGT]AGG 2977 CCTGAGG BalI 1101 TGGCCA TGG^CCA 2789 TGGCCA BamHI 1386 GGATCC G^GATCC 1519 GGATCC BbeI 344 GGCGCC GGCGC^C 1263 GGCGCC BbvCI 4161 CCTCAGC CCTCAGC(-5/-2) 3339 GCTGAGG BcgI 2766 GCAACAGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3171 GCAGCTCAATCG BlpI 2994 GCTAAGC GC^T[ACGT]AGC 4131 GCTCAGC Bpu1102I 2994 GCTAAGC GC^T[ACGT]AGC 4131 GCTCAGC BsaHI 344 GGCGCC G[GA]^CG[CT]C 1263 GGCGCC BsaI 1772 GAGACC GGTCTC(1/5) 2698 GAGACC BseAI 2954 TCCGGA T^CCGGA 3206 TCCGGA BspEI 2954 TCCGGA T^CCGGA 3206 TCCGGA BstAPI 1865 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3402 GCAACTGGTGC BstXI 2702 CCAAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3470 CCAGTGTTCTGG Bsu36I 807 CCTGAGG CC^T[ACGT]AGG 2977 CCTGAGG CelII 2994 GCTAAGC GC^T[ACGT]AGC 4131 GCTCAGC EagI 261 CGGCCG C^GGCCG 3437 CGGCCG EclXI 261 CGGCCG C^GGCCG 3437 CGGCCG Eco52I 261 CGGCCG C^GGCCG 3437 CGGCCG Eco81I 807 CCTGAGG CC^T[ACGT]AGG 2977 CCTGAGG EcoNI 589 CCTATGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1268 CCTGTGTCAGG EcoRI 1922 GAATTC G^AATTC 2187 GAATTC Hin1I 344 GGCGCC G[GA]^CG[CT]C 1263 GGCGCC HincII 1176 GTTGAC GT[CT]^[GA]AC 1646 GTTAAC HindII 1176 GTTGAC GT[CT]^[GA]AC 1646 GTTAAC KasI 344 GGCGCC G^GCGCC 1263 GGCGCC MaeII 233 ACGT A^CGT 4349 ACGT MluNI 1101 TGGCCA TGG^CCA 2789 TGGCCA MroI 2954 TCCGGA T^CCGGA 3206 TCCGGA MscI 1101 TGGCCA TGG^CCA 2789 TGGCCA NarI 344 GGCGCC GG^CGCC 1263 GGCGCC NspI 896 GCATGC [GA]CATG^[CT] 3492 ACATGC PaeR7I 1614 CTCGAG C^TCGAG 3485 CTCGAG SbfI 1715 CCTGCAGG CCTGCA^GG 3117 CCTGCAGG SfoI 344 GGCGCC GGC^GCC 1263 GGCGCC Sse8387I 1715 CCTGCAGG CCTGCA^GG 3117 CCTGCAGG XhoI 1614 CTCGAG C^TCGAG 3485 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NheI NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII XmaCI XmaI