##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BamHI BbrPI BbvCI
BcgI BcnI BfrI BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI HpaI KpnI MamI
MluI MroI NciI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SalI SbfI SexAI SfiI SfuI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 599 GTCTAC GT^[AC][GT]AC
AcyI 2133 GGCGCC G[GA]^CG[CT]C
AhdI 792 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 955 GTGCAC G^TGCAC
ApaLI 955 GTGCAC G^TGCAC
AspEI 792 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2133 GGCGCC GGCGC^C
BclI 610 TGATCA T^GATCA
BglI 2176 GCCTGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 129 AGATCT A^GATCT
BsaHI 2133 GGCGCC G[GA]^CG[CT]C
BsmI 2091 GAATGC GAATGC(1/-1)
BspHI 706 TCATGA T^CATGA
BspMI 204 GCAGGT ACCTGC(4/8)
BstXI 2100 CCAACCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2581 GACTCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 792 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 560 CCTATCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 610 TGATCA T^GATCA
Hin1I 2133 GGCGCC G[GA]^CG[CT]C
HincII 1655 GTTGAC GT[CT]^[GA]AC
HindII 1655 GTTGAC GT[CT]^[GA]AC
KasI 2133 GGCGCC G^GCGCC
KspI 28 CCGCGG CCGC^GG
NaeI 2130 GCCGGC GCC^GGC
NarI 2133 GGCGCC GG^CGCC
NgoMIV 2130 GCCGGC G^CCGGC
NheI 1930 GCTAGC G^CTAGC
PflMI 2328 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1688 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 706 TCATGA T^CATGA
SacII 28 CCGCGG CCGC^GG
SapI 197 GAAGAGC GCTCTTC(1/4)
SfaNI 2434 GCATC GCATC(5/9)
SfoI 2133 GGCGCC GGC^GCC
SgrAI 2129 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2842 GCATGC GCATG^C
SspI 3274 AATATT AAT^ATT
Van91I 2328 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 29 CGCG CG^CG
1190 CGCG
AocI 881 CCTGAGG CC^T[ACGT]AGG
2925 CCTTAGG
Asp700I 382 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2005 GAAGAAGTTC
BanI 736 GGTGCC G^G[CT][GA]CC
2133 GGCGCC
BciVI 2768 GTATCC GTATCC(6/5)
3129 GTATCC
BsaI 761 GAGACC GGTCTC(1/5)
2623 GGTCTC
BsrBI 23 CCGCTC CCGCTC(-3/-3)
1567 CCGCTC
BsrFI 2130 GCCGGC [GA]^CCGG[CT]
2181 ACCGGC
BstUI 29 CGCG CG^CG
1190 CGCG
BstYI 129 AGATCT [GA]^GATC[CT]
355 GGATCT
Bsu36I 881 CCTGAGG CC^T[ACGT]AGG
2925 CCTTAGG
Cfr10I 2130 GCCGGC [GA]^CCGG[CT]
2181 ACCGGC
EarI 77 CTCTTC CTCTTC(1/4)
197 GAAGAG
Eco81I 881 CCTGAGG CC^T[ACGT]AGG
2925 CCTTAGG
FauI 210 CCCGC CCCGC(4/6)
30 GCGGG
HindIII 1385 AAGCTT A^AGCTT
2691 AAGCTT
Ksp632I 77 CTCTTC CTCTTC(1/4)
197 GAAGAG
MfeI 426 CAATTG C^AATTG
606 CAATTG
MflI 129 AGATCT [GA]^GATC[CT]
355 GGATCT
MunI 426 CAATTG C^AATTG
606 CAATTG
MvnI 29 CGCG CG^CG
1190 CGCG
ScaI 1215 AGTACT AGT^ACT
1911 AGTACT
StuI 73 AGGCCT AGG^CCT
1974 AGGCCT
XcmI 1810 CCAGGGGTCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2801 CCACGGCTATAGTGG
XhoII 129 AGATCT [GA]^GATC[CT]
355 GGATCT
XmnI 382 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2005 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI ApaI AspHI AvaII BalI
BanII BbsI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I