##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII BamHI BanI BbeI BbrPI
BcgI BglII BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BssHII BstAPI BstBI
ClaI CpoI DraIII DrdI Eam1105I
Eco47III EcoRI EcoT22I FseI FspI
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RsrII
SacI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 46 GACGTC GACGT^C
AcyI 46 GACGTC G[GA]^CG[CT]C
Alw44I 1816 GTGCAC G^TGCAC
AlwI 348 GATCC GGATC(4/5)
AocI 1956 CCTTAGG CC^T[ACGT]AGG
ApaI 328 GGGCCC GGGCC^C
ApaLI 1816 GTGCAC G^TGCAC
Asp700I 2463 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1816 GTGCAC G[AT]GC[AT]^C
AvaI 1315 CCCGGG C^[CT]CG[GA]G
AvrII 2365 CCTAGG C^CTAGG
BalI 1122 TGGCCA TGG^CCA
BbvCI 3250 GCTGAGG CCTCAGC(-5/-2)
BclI 1661 TGATCA T^GATCA
BglI 3262 GCCTGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2365 CCTAGG C^CTAGG
BsaHI 46 GACGTC G[GA]^CG[CT]C
BsaI 1446 GGTCTC GGTCTC(1/5)
BsiEI 1159 CGGCCG CG[GA][CT]^CG
BsiHKAI 1816 GTGCAC G[AT]GC[AT]^C
BsoBI 1315 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2299 TGTACA T^GTACA
BspHI 2712 TCATGA T^CATGA
BsrDI 123 CATTGC GCAATG(2/0)
BsrFI 1 GCCGGC [GA]^CCGG[CT]
BsrGI 2299 TGTACA T^GTACA
BssSI 175 CACGAG CACGAG(-5/-1)
BstEII 2241 GGTGACC G^GT[ACGT]ACC
BstPI 2241 GGTGACC G^GT[ACGT]ACC
BstYI 347 AGATCC [GA]^GATC[CT]
Bsu36I 1956 CCTTAGG CC^T[ACGT]AGG
Cfr10I 1 GCCGGC [GA]^CCGG[CT]
EagI 1159 CGGCCG C^GGCCG
EclXI 1159 CGGCCG C^GGCCG
Eco52I 1159 CGGCCG C^GGCCG
Eco81I 1956 CCTTAGG CC^T[ACGT]AGG
EcoNI 403 CCTGACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2241 GGTGACC G^GT[ACGT]ACC
EcoRV 92 GATATC GAT^ATC
FbaI 1661 TGATCA T^GATCA
Hin1I 46 GACGTC G[GA]^CG[CT]C
HincII 295 GTCGAC GT[CT]^[GA]AC
HindII 295 GTCGAC GT[CT]^[GA]AC
KspI 1162 CCGCGG CCGC^GG
MflI 347 AGATCC [GA]^GATC[CT]
MluNI 1122 TGGCCA TGG^CCA
MscI 1122 TGGCCA TGG^CCA
MspA1I 1162 CCGCGG C[AC]G^C[GT]G
NaeI 1 GCCGGC GCC^GGC
NdeI 3341 CATATG CA^TATG
NgoMIV 1 GCCGGC G^CCGGC
RcaI 2712 TCATGA T^CATGA
SacII 1162 CCGCGG CCGC^GG
SalI 295 GTCGAC G^TCGAC
SmaI 1315 CCCGGG CCC^GGG
SspBI 2299 TGTACA T^GTACA
XbaI 1886 TCTAGA T^CTAGA
XhoII 347 AGATCC [GA]^GATC[CT]
XmaCI 1315 CCCGGG C^CCGGG
XmaI 1315 CCCGGG C^CCGGG
XmnI 2463 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 360 CGCG CG^CG
1163 CGCG
AflII 589 CTTAAG C^TTAAG
2971 CTTAAG
AseI 2336 ATTAAT AT^TAAT
2723 ATTAAT
AsnI 2336 ATTAAT AT^TAAT
2723 ATTAAT
AspI 2705 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3350 GACCCTGTC
BfrI 589 CTTAAG C^TTAAG
2971 CTTAAG
BsmI 1405 GAATGC GAATGC(1/-1)
577 GCATTC
BspMI 544 ACCTGC ACCTGC(4/8)
3411 ACCTGC
Bst1107I 211 GTATAC GTA^TAC
519 GTATAC
BstUI 360 CGCG CG^CG
1163 CGCG
BstXI 1191 CCACAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2278 CCAGAGTTCTGG
BstZ17I 211 GTATAC GTA^TAC
519 GTATAC
CfoI 180 GCGC GCG^C
1265 GCGC
FauI 949 CCCGC CCCGC(4/6)
1164 GCGGG
HaeII 179 AGCGCC [GA]GCGC^[CT]
1264 GGCGCT
HgaI 358 GACGC GACGC(5/10)
1222 GACGC
HhaI 180 GCGC GCG^C
1265 GCGC
HinP1I 180 GCGC G^CGC
1265 GCGC
HindIII 2560 AAGCTT A^AGCTT
2832 AAGCTT
MaeII 47 ACGT A^CGT
1802 ACGT
MvnI 360 CGCG CG^CG
1163 CGCG
NcoI 1758 CCATGG C^CATGG
3257 CCATGG
NspI 994 ACATGC [GA]CATG^[CT]
3439 GCATGT
PflFI 2705 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3350 GACCCTGTC
PflMI 1097 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1578 CCATTGTTTGG
PshBI 2336 ATTAAT AT^TAAT
2723 ATTAAT
PstI 486 CTGCAG CTGCA^G
546 CTGCAG
SpeI 1671 ACTAGT A^CTAGT
2348 ACTAGT
SspI 2902 AATATT AAT^ATT
3463 AATATT
Tth111I 2705 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3350 GACCCTGTC
Van91I 1097 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1578 CCATTGTTTGG
XcmI 1426 CCAACAGCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3257 CCATGGCCTGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwNI ApoI AvaII BanII BbsI
BbvI BciVI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CelII Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I