##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI AlwNI
ApaI AscI Asp700I Asp718I AspEI
AspI AvaI AvrII BbeI BbrPI
BclI BcnI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI Hin1I HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI SwaI
Tth111I Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Alw44I 489 GTGCAC G^TGCAC
AocI 183 CCTAAGG CC^T[ACGT]AGG
Aor51HI 919 AGCGCT AGC^GCT
ApaLI 489 GTGCAC G^TGCAC
AseI 1047 ATTAAT AT^TAAT
AsnI 1047 ATTAAT AT^TAAT
AviII 1246 TGCGCA TGC^GCA
BamHI 1432 GGATCC G^GATCC
BanI 1724 GGCACC G^G[CT][GA]CC
BbsI 288 GAAGAC GAAGAC(2/6)
BbvCI 194 GCTGAGG CCTCAGC(-5/-2)
BcgI 881 CGAGCGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 665 GGATAC GTATCC(6/5)
BlpI 848 GCTGAGC GC^T[ACGT]AGC
BpmI 1525 CTGGAG CTGGAG(16/14)
Bpu1102I 848 GCTGAGC GC^T[ACGT]AGC
BpuAI 288 GAAGAC GAAGAC(2/6)
BsaWI 701 TCCGGT [AT]^CCGG[AT]
BsmFI 2117 GTCCC GGGAC(10/14)
BsmI 101 GCATTC GAATGC(1/-1)
BssSI 1269 CACGAG CACGAG(-5/-1)
BstXI 1186 CCACTCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1432 GGATCC [GA]^GATC[CT]
Bsu36I 183 CCTAAGG CC^T[ACGT]AGG
CelII 848 GCTGAGC GC^T[ACGT]AGC
DraII 63 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 3127 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 919 AGCGCT AGC^GCT
Eco81I 183 CCTAAGG CC^T[ACGT]AGG
EcoO109I 63 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 2939 ATGCAT ATGCA^T
FauI 142 GCGGG CCCGC(4/6)
FspI 1246 TGCGCA TGC^GCA
HapII 702 CCGG C^CGG
HgaI 1093 GCGTC GACGC(5/10)
HpaII 702 CCGG C^CGG
MfeI 1876 CAATTG C^AATTG
MflI 1432 GGATCC [GA]^GATC[CT]
MslI 1473 CACCTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 702 CCGG C^CGG
MunI 1876 CAATTG C^AATTG
NsiI 2939 ATGCAT ATGCA^T
PpuMI 63 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1733 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1047 ATTAAT AT^TAAT
PstI 9 CTGCAG CTGCA^G
PvuII 1522 CAGCTG CAG^CTG
ScaI 2401 AGTACT AGT^ACT
SfcI 9 CTGCAG C^T[GA][CT]AG
SpeI 2732 ACTAGT A^CTAGT
StuI 1745 AGGCCT AGG^CCT
XbaI 2109 TCTAGA T^CTAGA
XcmI 2458 CCAAGTTGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1432 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 601 GTCTAC GT^[AC][GT]AC
2382 GTCTAC
AflII 915 CTTAAG C^TTAAG
2208 CTTAAG
BalI 117 TGGCCA TGG^CCA
1607 TGGCCA
BanII 1041 GAGCTC G[GA]GC[CT]^C
1680 GAGCTC
BfrI 915 CTTAAG C^TTAAG
2208 CTTAAG
BseRI 1207 GAGGAG GAGGAG(10/8)
1630 GAGGAG
BsgI 923 CTGCAC GTGCAG(16/14)
1986 GTGCAG
BsmAI 1217 GAGAC GTCTC(1/5)
2042 GTCTC
DrdI 2640 GACACATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2849 GACTGTCAGGTC
EaeI 117 TGGCCA [CT]^GGCC[GA]
1607 TGGCCA
HaeII 883 AGCGCC [GA]GCGC^[CT]
919 AGCGCT
HincII 813 GTCAAC GT[CT]^[GA]AC
2649 GTCAAC
HindII 813 GTCAAC GT[CT]^[GA]AC
2649 GTCAAC
MluNI 117 TGGCCA TGG^CCA
1607 TGGCCA
MscI 117 TGGCCA TGG^CCA
1607 TGGCCA
MspA1I 1023 CCGCTG C[AC]G^C[GT]G
1522 CAGCTG
NcoI 1291 CCATGG C^CATGG
3176 CCATGG
NspI 1249 GCATGT [GA]CATG^[CT]
1756 GCATGT
SacI 1041 GAGCTC GAGCT^C
1680 GAGCTC
SmlI 915 CTTAAG C^T[CT][GA]AG
2208 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I Bsp1407I BspHI BsrDI BsrGI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I