##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
BbeI BcgI BciVI BsaBI BsiWI
BspDI BspLU11I BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI HpaI KasI KpnI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1270 GTCTAC GT^[AC][GT]AC
AccIII 1844 TCCGGA T^CCGGA
AcsI 2119 GAATTC [GA]^AATT[CT]
AcyI 33 GGCGTC G[GA]^CG[CT]C
AflII 3482 CTTAAG C^TTAAG
AhdI 3323 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2119 GAATTC [GA]^AATT[CT]
AspEI 3323 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1120 TGCGCA TGC^GCA
AvrII 396 CCTAGG C^CTAGG
BamHI 410 GGATCC G^GATCC
BclI 2622 TGATCA T^GATCA
BfrI 3482 CTTAAG C^TTAAG
BglII 731 AGATCT A^GATCT
BlnI 396 CCTAGG C^CTAGG
BsaHI 33 GGCGTC G[GA]^CG[CT]C
BseAI 1844 TCCGGA T^CCGGA
BsiEI 257 CGGTCG CG[GA][CT]^CG
BsmBI 2170 GAGACG CGTCTC(1/5)
BspEI 1844 TCCGGA T^CCGGA
BsrDI 1970 GCAATG GCAATG(2/0)
BsrFI 1703 ACCGGC [GA]^CCGG[CT]
BssSI 890 CACGAG CACGAG(-5/-1)
BstXI 2684 CCACACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1703 ACCGGC [GA]^CCGG[CT]
DrdI 1581 GACCTCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3323 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2119 GAATTC G^AATTC
FbaI 2622 TGATCA T^GATCA
FspI 1120 TGCGCA TGC^GCA
HaeII 3425 GGCGCT [GA]GCGC^[CT]
Hin1I 33 GGCGTC G[GA]^CG[CT]C
HindIII 2371 AAGCTT A^AGCTT
MroI 1844 TCCGGA T^CCGGA
NcoI 312 CCATGG C^CATGG
PaeR7I 2454 CTCGAG C^TCGAG
SexAI 3552 ACCAGGT A^CC[AT]GGT
XhoI 2454 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BbrPI 2626 CACGTG CAC^GTG
3458 CACGTG
BbsI 1923 GAAGAC GAAGAC(2/6)
2449 GAAGAC
BpuAI 1923 GAAGAC GAAGAC(2/6)
2449 GAAGAC
BsaAI 2626 CACGTG [CT]AC^GT[GA]
3458 CACGTG
BsaI 921 GAGACC GGTCTC(1/5)
1789 GGTCTC
BsaWI 768 ACCGGA [AT]^CCGG[AT]
1844 TCCGGA
BsmI 1681 GAATGC GAATGC(1/-1)
3692 GCATTC
Bsp1407I 2499 TGTACA T^GTACA
3256 TGTACA
BsrGI 2499 TGTACA T^GTACA
3256 TGTACA
BstAPI 1446 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2968 GCACCTTATGC
CfoI 1121 GCGC GCG^C
3426 GCGC
DraIII 1614 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3551 CACCAGGTG
HgaI 34 GCGTC GACGC(5/10)
1431 GCGTC
HhaI 1121 GCGC GCG^C
3426 GCGC
HinP1I 1121 GCGC G^CGC
3426 GCGC
KspI 3584 CCGCGG CCGC^GG
3977 CCGCGG
NruI 2004 TCGCGA TCG^CGA
3150 TCGCGA
NspI 319 ACATGC [GA]CATG^[CT]
2309 ACATGC
PmaCI 2626 CACGTG CAC^GTG
3458 CACGTG
PmlI 2626 CACGTG CAC^GTG
3458 CACGTG
SacII 3584 CCGCGG CCGC^GG
3977 CCGCGG
SmaI 3539 CCCGGG CCC^GGG
3777 CCCGGG
SspBI 2499 TGTACA T^GTACA
3256 TGTACA
XmaCI 3539 CCCGGG C^CCGGG
3777 CCCGGG
XmaI 3539 CCCGGG C^CCGGG
3777 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII