################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AccI AccIII AclI AflIII AgeI AscI Asp700I AvrII BbeI BbrPI BcgI BciVI BclI BlnI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoO65I EcoT22I FbaI FseI HindIII HpaI KasI KspI MfeI MluI MroI MunI NarI NdeI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfoI SfuI SnaBI SphI Sse8387I SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 848 GACGTC GACGT^C Acc65I 905 GGTACC G^GTACC AccII 1197 CGCG CG^CG AflII 2451 CTTAAG C^TTAAG Alw44I 1790 GTGCAC G^TGCAC Aor51HI 333 AGCGCT AGC^GCT ApaLI 1790 GTGCAC G^TGCAC AseI 3366 ATTAAT AT^TAAT AsnI 3366 ATTAAT AT^TAAT Asp718I 905 GGTACC G^GTACC AspI 217 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BfrI 2451 CTTAAG C^TTAAG BsaBI 2006 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1567 TCCGGT [AT]^CCGG[AT] BsmBI 2706 CGTCTC CGTCTC(1/5) Bsp1407I 2910 TGTACA T^GTACA BsrBI 479 CCGCTC CCGCTC(-3/-3) BsrGI 2910 TGTACA T^GTACA BstUI 1197 CGCG CG^CG DrdI 842 GACTTTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 333 AGCGCT AGC^GCT EcoNI 665 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 752 GATATC GAT^ATC FauI 2345 GCGGG CCCGC(4/6) KpnI 905 GGTACC GGTAC^C MamI 2006 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 1197 CGCG CG^CG NaeI 954 GCCGGC GCC^GGC NgoMIV 954 GCCGGC G^CCGGC NheI 653 GCTAGC G^CTAGC PaeR7I 92 CTCGAG C^TCGAG PflFI 217 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2990 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 3366 ATTAAT AT^TAAT PstI 1650 CTGCAG CTGCA^G SacI 1482 GAGCTC GAGCT^C SapI 1667 GCTCTTC GCTCTTC(1/4) SexAI 607 ACCTGGT A^CC[AT]GGT SfiI 1631 GGCCTACTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 953 CGCCGGCG C[GA]^CCGG[CT]G SmaI 67 CCCGGG CCC^GGG SpeI 3113 ACTAGT A^CTAGT SspBI 2910 TGTACA T^GTACA SspI 3301 AATATT AAT^ATT Tth111I 217 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2990 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 92 CTCGAG C^TCGAG XmaCI 67 CCCGGG C^CCGGG XmaI 67 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 301 GAATTC [GA]^AATT[CT] 2732 GAATTC ApoI 301 GAATTC [GA]^AATT[CT] 2732 GAATTC AviII 806 TGCGCA TGC^GCA 1159 TGCGCA BamHI 1012 GGATCC G^GATCC 2167 GGATCC BbsI 506 GAAGAC GAAGAC(2/6) 1335 GAAGAC BglI 1632 GCCTACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2658 GCCACAGTGGC BglII 1539 AGATCT A^GATCT 2992 AGATCT BpuAI 506 GAAGAC GAAGAC(2/6) 1335 GAAGAC BsaAI 1249 TACGTG [CT]AC^GT[GA] 2547 CACGTA BspMI 201 ACCTGC ACCTGC(4/8) 1176 ACCTGC BsrFI 954 GCCGGC [GA]^CCGG[CT] 2252 GCCGGT BssSI 1095 CACGAG CACGAG(-5/-1) 1124 CTCGTG Cfr10I 954 GCCGGC [GA]^CCGG[CT] 2252 GCCGGT DraIII 2593 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2838 CACAGGGTG EcoRI 301 GAATTC G^AATTC 2732 GAATTC FspI 806 TGCGCA TGC^GCA 1159 TGCGCA HgaI 1364 GACGC GACGC(5/10) 1053 GCGTC HincII 452 GTTGAC GT[CT]^[GA]AC 932 GTCAAC HindII 452 GTTGAC GT[CT]^[GA]AC 932 GTCAAC NspI 803 ACATGC [GA]CATG^[CT] 2923 GCATGT PpuMI 397 GGGACCC [GA]G^G[AT]CC[CT] 981 AGGACCC TaqI 93 TCGA T^CGA 2678 TCGA TthHB8I 93 TCGA T^CGA 2678 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcyI AfaI AhdI AluI AlwI AlwNI AocI ApaI AspEI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII