##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccI AccIII AclI AflIII AgeI
AscI Asp700I AvrII BbeI BbrPI
BcgI BciVI BclI BlnI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoO65I EcoT22I FbaI FseI HindIII
HpaI KasI KspI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfoI SfuI SnaBI
SphI Sse8387I SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 848 GACGTC GACGT^C
Acc65I 905 GGTACC G^GTACC
AccII 1197 CGCG CG^CG
AflII 2451 CTTAAG C^TTAAG
Alw44I 1790 GTGCAC G^TGCAC
Aor51HI 333 AGCGCT AGC^GCT
ApaLI 1790 GTGCAC G^TGCAC
AseI 3366 ATTAAT AT^TAAT
AsnI 3366 ATTAAT AT^TAAT
Asp718I 905 GGTACC G^GTACC
AspI 217 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfrI 2451 CTTAAG C^TTAAG
BsaBI 2006 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1567 TCCGGT [AT]^CCGG[AT]
BsmBI 2706 CGTCTC CGTCTC(1/5)
Bsp1407I 2910 TGTACA T^GTACA
BsrBI 479 CCGCTC CCGCTC(-3/-3)
BsrGI 2910 TGTACA T^GTACA
BstUI 1197 CGCG CG^CG
DrdI 842 GACTTTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 333 AGCGCT AGC^GCT
EcoNI 665 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 752 GATATC GAT^ATC
FauI 2345 GCGGG CCCGC(4/6)
KpnI 905 GGTACC GGTAC^C
MamI 2006 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1197 CGCG CG^CG
NaeI 954 GCCGGC GCC^GGC
NgoMIV 954 GCCGGC G^CCGGC
NheI 653 GCTAGC G^CTAGC
PaeR7I 92 CTCGAG C^TCGAG
PflFI 217 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2990 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3366 ATTAAT AT^TAAT
PstI 1650 CTGCAG CTGCA^G
SacI 1482 GAGCTC GAGCT^C
SapI 1667 GCTCTTC GCTCTTC(1/4)
SexAI 607 ACCTGGT A^CC[AT]GGT
SfiI 1631 GGCCTACTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 953 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 67 CCCGGG CCC^GGG
SpeI 3113 ACTAGT A^CTAGT
SspBI 2910 TGTACA T^GTACA
SspI 3301 AATATT AAT^ATT
Tth111I 217 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2990 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 92 CTCGAG C^TCGAG
XmaCI 67 CCCGGG C^CCGGG
XmaI 67 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 301 GAATTC [GA]^AATT[CT]
2732 GAATTC
ApoI 301 GAATTC [GA]^AATT[CT]
2732 GAATTC
AviII 806 TGCGCA TGC^GCA
1159 TGCGCA
BamHI 1012 GGATCC G^GATCC
2167 GGATCC
BbsI 506 GAAGAC GAAGAC(2/6)
1335 GAAGAC
BglI 1632 GCCTACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2658 GCCACAGTGGC
BglII 1539 AGATCT A^GATCT
2992 AGATCT
BpuAI 506 GAAGAC GAAGAC(2/6)
1335 GAAGAC
BsaAI 1249 TACGTG [CT]AC^GT[GA]
2547 CACGTA
BspMI 201 ACCTGC ACCTGC(4/8)
1176 ACCTGC
BsrFI 954 GCCGGC [GA]^CCGG[CT]
2252 GCCGGT
BssSI 1095 CACGAG CACGAG(-5/-1)
1124 CTCGTG
Cfr10I 954 GCCGGC [GA]^CCGG[CT]
2252 GCCGGT
DraIII 2593 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2838 CACAGGGTG
EcoRI 301 GAATTC G^AATTC
2732 GAATTC
FspI 806 TGCGCA TGC^GCA
1159 TGCGCA
HgaI 1364 GACGC GACGC(5/10)
1053 GCGTC
HincII 452 GTTGAC GT[CT]^[GA]AC
932 GTCAAC
HindII 452 GTTGAC GT[CT]^[GA]AC
932 GTCAAC
NspI 803 ACATGC [GA]CATG^[CT]
2923 GCATGT
PpuMI 397 GGGACCC [GA]G^G[AT]CC[CT]
981 AGGACCC
TaqI 93 TCGA T^CGA
2678 TCGA
TthHB8I 93 TCGA T^CGA
2678 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcyI AfaI AhdI AluI
AlwI AlwNI AocI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII