################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp718I AspEI AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BspEI BspLU11I BsrBI BssHII BstAPI BstBI BstEII BstPI BstXI Bsu36I CelII CpoI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I FseI KasI KpnI MluI MluNI MroI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SalI SbfI SexAI SfoI SfuI SmaI SnaBI SphI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 1719 GACGTC GACGT^C AccI 1922 GTATAC GT^[AC][GT]AC Aor51HI 799 AGCGCT AGC^GCT Asp700I 2463 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2244 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3951 CTCGAG C^[CT]CG[GA]G AviII 794 TGCGCA TGC^GCA BglI 87 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 3030 TACGTG [CT]AC^GT[GA] BsaBI 326 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3420 GGTCTC GGTCTC(1/5) BsgI 1704 CTGCAC GTGCAG(16/14) BsmBI 3272 GAGACG CGTCTC(1/5) BsmFI 1624 GGGAC GGGAC(10/14) BsmI 1010 GAATGC GAATGC(1/-1) BsoBI 3951 CTCGAG C^[CT]CG[GA]G BspDI 1219 ATCGAT AT^CGAT BspHI 1643 TCATGA T^CATGA BsrFI 72 ACCGGC [GA]^CCGG[CT] Bst1107I 1922 GTATAC GTA^TAC BstZ17I 1922 GTATAC GTA^TAC Cfr10I 72 ACCGGC [GA]^CCGG[CT] ClaI 1219 ATCGAT AT^CGAT DraI 1739 TTTAAA TTT^AAA DraII 479 GGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 1629 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 55 TGGCCG [CT]^GGCC[GA] Eco47III 799 AGCGCT AGC^GCT EcoO109I 479 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 1267 GAATTC G^AATTC EcoT14I 787 CCATGG C^C[AT][AT]GG EcoT22I 2814 ATGCAT ATGCA^T FspI 794 TGCGCA TGC^GCA HaeII 799 AGCGCT [GA]GCGC^[CT] HgaI 2390 GCGTC GACGC(5/10) HindIII 713 AAGCTT A^AGCTT HpaI 3942 GTTAAC GTT^AAC KspI 91 CCGCGG CCGC^GG MamI 326 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3532 CAATTG C^AATTG MunI 3532 CAATTG C^AATTG NcoI 787 CCATGG C^CATGG NsiI 2814 ATGCAT ATGCA^T NspI 3384 GCATGT [GA]CATG^[CT] PaeR7I 3951 CTCGAG C^TCGAG PflFI 2244 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 479 GGGTCCT [GA]G^G[AT]CC[CT] RcaI 1643 TCATGA T^CATGA SacII 91 CCGCGG CCGC^GG SapI 613 GCTCTTC GCTCTTC(1/4) ScaI 766 AGTACT AGT^ACT SfiI 86 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 71 CACCGGCG C[GA]^CCGG[CT]G SpeI 971 ACTAGT A^CTAGT StyI 787 CCATGG C^C[AT][AT]GG Tth111I 2244 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1815 CCAAATACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3951 CTCGAG C^TCGAG XmnI 2463 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcyI 1719 GACGTC G[GA]^CG[CT]C 2389 GGCGTC AseI 2957 ATTAAT AT^TAAT 4056 ATTAAT AsnI 2957 ATTAAT AT^TAAT 4056 ATTAAT BcnI 17 CCGGG CC^[GC]GG 2335 CCCGG BsaHI 1719 GACGTC G[GA]^CG[CT]C 2389 GGCGTC BseRI 2424 GAGGAG GAGGAG(10/8) 3269 GAGGAG Bsp1407I 1349 TGTACA T^GTACA 3674 TGTACA BspMI 1682 ACCTGC ACCTGC(4/8) 63 GCAGGT BsrGI 1349 TGTACA T^GTACA 3674 TGTACA BssSI 739 CACGAG CACGAG(-5/-1) 3769 CACGAG EcoRV 256 GATATC GAT^ATC 752 GATATC FauI 906 CCCGC CCCGC(4/6) 44 GCGGG Hin1I 1719 GACGTC G[GA]^CG[CT]C 2389 GGCGTC NciI 17 CCGGG CC^[GC]GG 2335 CCCGG PshBI 2957 ATTAAT AT^TAAT 4056 ATTAAT SspBI 1349 TGTACA T^GTACA 3674 TGTACA StuI 1787 AGGCCT AGG^CCT 2744 AGGCCT XbaI 1194 TCTAGA T^CTAGA 2088 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BclI BfaI BglII BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PflMI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII