##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BlnI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BspEI BspLU11I BsrBI BssHII
BstAPI BstBI BstEII BstPI BstXI
Bsu36I CelII CpoI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I FseI KasI KpnI MluI
MluNI MroI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RsrII SalI SbfI SexAI SfoI
SfuI SmaI SnaBI SphI Sse8387I
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 1719 GACGTC GACGT^C
AccI 1922 GTATAC GT^[AC][GT]AC
Aor51HI 799 AGCGCT AGC^GCT
Asp700I 2463 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2244 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3951 CTCGAG C^[CT]CG[GA]G
AviII 794 TGCGCA TGC^GCA
BglI 87 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3030 TACGTG [CT]AC^GT[GA]
BsaBI 326 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3420 GGTCTC GGTCTC(1/5)
BsgI 1704 CTGCAC GTGCAG(16/14)
BsmBI 3272 GAGACG CGTCTC(1/5)
BsmFI 1624 GGGAC GGGAC(10/14)
BsmI 1010 GAATGC GAATGC(1/-1)
BsoBI 3951 CTCGAG C^[CT]CG[GA]G
BspDI 1219 ATCGAT AT^CGAT
BspHI 1643 TCATGA T^CATGA
BsrFI 72 ACCGGC [GA]^CCGG[CT]
Bst1107I 1922 GTATAC GTA^TAC
BstZ17I 1922 GTATAC GTA^TAC
Cfr10I 72 ACCGGC [GA]^CCGG[CT]
ClaI 1219 ATCGAT AT^CGAT
DraI 1739 TTTAAA TTT^AAA
DraII 479 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1629 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 55 TGGCCG [CT]^GGCC[GA]
Eco47III 799 AGCGCT AGC^GCT
EcoO109I 479 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1267 GAATTC G^AATTC
EcoT14I 787 CCATGG C^C[AT][AT]GG
EcoT22I 2814 ATGCAT ATGCA^T
FspI 794 TGCGCA TGC^GCA
HaeII 799 AGCGCT [GA]GCGC^[CT]
HgaI 2390 GCGTC GACGC(5/10)
HindIII 713 AAGCTT A^AGCTT
HpaI 3942 GTTAAC GTT^AAC
KspI 91 CCGCGG CCGC^GG
MamI 326 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3532 CAATTG C^AATTG
MunI 3532 CAATTG C^AATTG
NcoI 787 CCATGG C^CATGG
NsiI 2814 ATGCAT ATGCA^T
NspI 3384 GCATGT [GA]CATG^[CT]
PaeR7I 3951 CTCGAG C^TCGAG
PflFI 2244 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 479 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1643 TCATGA T^CATGA
SacII 91 CCGCGG CCGC^GG
SapI 613 GCTCTTC GCTCTTC(1/4)
ScaI 766 AGTACT AGT^ACT
SfiI 86 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 71 CACCGGCG C[GA]^CCGG[CT]G
SpeI 971 ACTAGT A^CTAGT
StyI 787 CCATGG C^C[AT][AT]GG
Tth111I 2244 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1815 CCAAATACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3951 CTCGAG C^TCGAG
XmnI 2463 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 1719 GACGTC G[GA]^CG[CT]C
2389 GGCGTC
AseI 2957 ATTAAT AT^TAAT
4056 ATTAAT
AsnI 2957 ATTAAT AT^TAAT
4056 ATTAAT
BcnI 17 CCGGG CC^[GC]GG
2335 CCCGG
BsaHI 1719 GACGTC G[GA]^CG[CT]C
2389 GGCGTC
BseRI 2424 GAGGAG GAGGAG(10/8)
3269 GAGGAG
Bsp1407I 1349 TGTACA T^GTACA
3674 TGTACA
BspMI 1682 ACCTGC ACCTGC(4/8)
63 GCAGGT
BsrGI 1349 TGTACA T^GTACA
3674 TGTACA
BssSI 739 CACGAG CACGAG(-5/-1)
3769 CACGAG
EcoRV 256 GATATC GAT^ATC
752 GATATC
FauI 906 CCCGC CCCGC(4/6)
44 GCGGG
Hin1I 1719 GACGTC G[GA]^CG[CT]C
2389 GGCGTC
NciI 17 CCGGG CC^[GC]GG
2335 CCCGG
PshBI 2957 ATTAAT AT^TAAT
4056 ATTAAT
SspBI 1349 TGTACA T^GTACA
3674 TGTACA
StuI 1787 AGGCCT AGG^CCT
2744 AGGCCT
XbaI 1194 TCTAGA T^CTAGA
2088 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BclI BfaI
BglII BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EarI EcoRII
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PflMI PleI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII