################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII Acc65I AclI AhdI Alw44I AlwI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BbvCI BciVI BclI BsiWI BspDI BspHI BsrDI BssHII BstBI BstEII BstPI ClaI CpoI DraI Eam1105I EcoO65I EcoT22I FbaI FseI FspI HpaI KpnI MfeI MluI MunI NheI NruI NsiI NspV PacI PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfuI SnaBI Sse8387I SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 677 GTATAC GT^[AC][GT]AC AccIII 739 TCCGGA T^CCGGA AflII 360 CTTAAG C^TTAAG AflIII 1201 ACATGT A^C[GA][CT]GT AgeI 2112 ACCGGT A^CCGGT Aor51HI 817 AGCGCT AGC^GCT AvrII 452 CCTAGG C^CTAGG BbeI 3394 GGCGCC GGCGC^C BbrPI 1572 CACGTG CAC^GTG BcgI 69 CGAGGTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 360 CTTAAG C^TTAAG BglII 793 AGATCT A^GATCT BlnI 452 CCTAGG C^CTAGG BsaBI 2535 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 739 TCCGGA T^CCGGA BsmBI 570 CGTCTC CGTCTC(1/5) BspEI 739 TCCGGA T^CCGGA BspLU11I 1201 ACATGT A^CATGT BsrBI 3087 GAGCGG CCGCTC(-3/-3) BssSI 207 CACGAG CACGAG(-5/-1) Bst1107I 677 GTATAC GTA^TAC BstYI 793 AGATCT [GA]^GATC[CT] BstZ17I 677 GTATAC GTA^TAC Eco47III 817 AGCGCT AGC^GCT EcoRV 1020 GATATC GAT^ATC HindIII 1117 AAGCTT A^AGCTT KasI 3394 GGCGCC G^GCGCC MamI 2535 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 793 AGATCT [GA]^GATC[CT] MroI 739 TCCGGA T^CCGGA NarI 3394 GGCGCC GG^CGCC NdeI 1061 CATATG CA^TATG NotI 137 GCGGCCGC GC^GGCCGC PinAI 2112 ACCGGT A^CCGGT PmaCI 1572 CACGTG CAC^GTG PmlI 1572 CACGTG CAC^GTG SacI 1221 GAGCTC GAGCT^C SapI 1401 GAAGAGC GCTCTTC(1/4) SexAI 343 ACCTGGT A^CC[AT]GGT SfoI 3394 GGCGCC GGC^GCC SgrAI 2111 CACCGGTG C[GA]^CCGG[CT]G XhoII 793 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Asp700I 109 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 495 GAACGCTTTC BsaAI 948 CACGTA [CT]AC^GT[GA] 1572 CACGTG BsaI 876 GAGACC GGTCTC(1/5) 975 GAGACC BsiEI 4 CGGCCG CG[GA][CT]^CG 138 CGGCCG BsmI 3945 GAATGC GAATGC(1/-1) 3783 GCATTC Bsp1407I 349 TGTACA T^GTACA 3051 TGTACA BspMI 2040 ACCTGC ACCTGC(4/8) 857 GCAGGT BsrGI 349 TGTACA T^GTACA 3051 TGTACA BstAPI 1767 GCATATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3674 GCAGAGCCTGC DraIII 2798 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 3997 CACACAGTG EagI 4 CGGCCG C^GGCCG 138 CGGCCG EarI 873 GAAGAG CTCTTC(1/4) 1401 GAAGAG EclXI 4 CGGCCG C^GGCCG 138 CGGCCG Eco52I 4 CGGCCG C^GGCCG 138 CGGCCG HgaI 193 GCGTC GACGC(5/10) 569 GCGTC Ksp632I 873 GAAGAG CTCTTC(1/4) 1401 GAAGAG KspI 7 CCGCGG CCGC^GG 3972 CCGCGG MslI 1568 CACCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2651 CATCACTGTG NaeI 2 GCCGGC GCC^GGC 3802 GCCGGC NgoMIV 2 GCCGGC G^CCGGC 3802 GCCGGC PflMI 1004 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2993 CCAGGAAGTGG SacII 7 CCGCGG CCGC^GG 3972 CCGCGG SpeI 2073 ACTAGT A^CTAGT 3326 ACTAGT SphI 1436 GCATGC GCATG^C 2663 GCATGC SspBI 349 TGTACA T^GTACA 3051 TGTACA Van91I 1004 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2993 CCAGGAAGTGG XmnI 109 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 495 GAACGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI XmaCI XmaI