##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AhdI Alw44I
AlwI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BbvCI BciVI BclI BsiWI BspDI
BspHI BsrDI BssHII BstBI BstEII
BstPI ClaI CpoI DraI Eam1105I
EcoO65I EcoT22I FbaI FseI FspI
HpaI KpnI MfeI MluI MunI
NheI NruI NsiI NspV PacI
PflFI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfiI SfuI SnaBI Sse8387I
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 677 GTATAC GT^[AC][GT]AC
AccIII 739 TCCGGA T^CCGGA
AflII 360 CTTAAG C^TTAAG
AflIII 1201 ACATGT A^C[GA][CT]GT
AgeI 2112 ACCGGT A^CCGGT
Aor51HI 817 AGCGCT AGC^GCT
AvrII 452 CCTAGG C^CTAGG
BbeI 3394 GGCGCC GGCGC^C
BbrPI 1572 CACGTG CAC^GTG
BcgI 69 CGAGGTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 360 CTTAAG C^TTAAG
BglII 793 AGATCT A^GATCT
BlnI 452 CCTAGG C^CTAGG
BsaBI 2535 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 739 TCCGGA T^CCGGA
BsmBI 570 CGTCTC CGTCTC(1/5)
BspEI 739 TCCGGA T^CCGGA
BspLU11I 1201 ACATGT A^CATGT
BsrBI 3087 GAGCGG CCGCTC(-3/-3)
BssSI 207 CACGAG CACGAG(-5/-1)
Bst1107I 677 GTATAC GTA^TAC
BstYI 793 AGATCT [GA]^GATC[CT]
BstZ17I 677 GTATAC GTA^TAC
Eco47III 817 AGCGCT AGC^GCT
EcoRV 1020 GATATC GAT^ATC
HindIII 1117 AAGCTT A^AGCTT
KasI 3394 GGCGCC G^GCGCC
MamI 2535 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 793 AGATCT [GA]^GATC[CT]
MroI 739 TCCGGA T^CCGGA
NarI 3394 GGCGCC GG^CGCC
NdeI 1061 CATATG CA^TATG
NotI 137 GCGGCCGC GC^GGCCGC
PinAI 2112 ACCGGT A^CCGGT
PmaCI 1572 CACGTG CAC^GTG
PmlI 1572 CACGTG CAC^GTG
SacI 1221 GAGCTC GAGCT^C
SapI 1401 GAAGAGC GCTCTTC(1/4)
SexAI 343 ACCTGGT A^CC[AT]GGT
SfoI 3394 GGCGCC GGC^GCC
SgrAI 2111 CACCGGTG C[GA]^CCGG[CT]G
XhoII 793 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Asp700I 109 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
495 GAACGCTTTC
BsaAI 948 CACGTA [CT]AC^GT[GA]
1572 CACGTG
BsaI 876 GAGACC GGTCTC(1/5)
975 GAGACC
BsiEI 4 CGGCCG CG[GA][CT]^CG
138 CGGCCG
BsmI 3945 GAATGC GAATGC(1/-1)
3783 GCATTC
Bsp1407I 349 TGTACA T^GTACA
3051 TGTACA
BspMI 2040 ACCTGC ACCTGC(4/8)
857 GCAGGT
BsrGI 349 TGTACA T^GTACA
3051 TGTACA
BstAPI 1767 GCATATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3674 GCAGAGCCTGC
DraIII 2798 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3997 CACACAGTG
EagI 4 CGGCCG C^GGCCG
138 CGGCCG
EarI 873 GAAGAG CTCTTC(1/4)
1401 GAAGAG
EclXI 4 CGGCCG C^GGCCG
138 CGGCCG
Eco52I 4 CGGCCG C^GGCCG
138 CGGCCG
HgaI 193 GCGTC GACGC(5/10)
569 GCGTC
Ksp632I 873 GAAGAG CTCTTC(1/4)
1401 GAAGAG
KspI 7 CCGCGG CCGC^GG
3972 CCGCGG
MslI 1568 CACCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2651 CATCACTGTG
NaeI 2 GCCGGC GCC^GGC
3802 GCCGGC
NgoMIV 2 GCCGGC G^CCGGC
3802 GCCGGC
PflMI 1004 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2993 CCAGGAAGTGG
SacII 7 CCGCGG CCGC^GG
3972 CCGCGG
SpeI 2073 ACTAGT A^CTAGT
3326 ACTAGT
SphI 1436 GCATGC GCATG^C
2663 GCATGC
SspBI 349 TGTACA T^GTACA
3051 TGTACA
Van91I 1004 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2993 CCAGGAAGTGG
XmnI 109 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
495 GAACGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PaeR7I PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoI XmaCI XmaI