##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccII AclI AgeI AocI
Aor51HI ApaI AscI AseI AsnI
AspI AviII BclI BsiEI BssHII
BstBI BstUI Bsu36I CpoI DraI
EagI EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FbaI FseI FspI
KspI MluI MvnI NaeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2993 GGTACC G^GTACC
AccIII 1919 TCCGGA T^CCGGA
AflII 694 CTTAAG C^TTAAG
AhdI 1241 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3852 GTGCAC G^TGCAC
ApaLI 3852 GTGCAC G^TGCAC
Asp700I 2676 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2993 GGTACC G^GTACC
AspEI 1241 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 852 CCTAGG C^CTAGG
BamHI 3057 GGATCC G^GATCC
BbeI 2039 GGCGCC GGCGC^C
BbrPI 2647 CACGTG CAC^GTG
BcgI 1737 CGATGAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 694 CTTAAG C^TTAAG
BlnI 852 CCTAGG C^CTAGG
BseAI 1919 TCCGGA T^CCGGA
BsiWI 1694 CGTACG C^GTACG
BsmI 3165 GAATGC GAATGC(1/-1)
BspDI 2317 ATCGAT AT^CGAT
BspEI 1919 TCCGGA T^CCGGA
BspLU11I 74 ACATGT A^CATGT
BssSI 178 CACGAG CACGAG(-5/-1)
Bst1107I 78 GTATAC GTA^TAC
BstZ17I 78 GTATAC GTA^TAC
ClaI 2317 ATCGAT AT^CGAT
DrdI 2206 GACAATGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1241 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1390 GAATTC G^AATTC
HindIII 2002 AAGCTT A^AGCTT
HpaI 1965 GTTAAC GTT^AAC
KasI 2039 GGCGCC G^GCGCC
KpnI 2993 GGTACC GGTAC^C
MfeI 4012 CAATTG C^AATTG
MroI 1919 TCCGGA T^CCGGA
MunI 4012 CAATTG C^AATTG
NarI 2039 GGCGCC GG^CGCC
NdeI 1573 CATATG CA^TATG
NheI 708 GCTAGC G^CTAGC
PmaCI 2647 CACGTG CAC^GTG
PmlI 2647 CACGTG CAC^GTG
ScaI 422 AGTACT AGT^ACT
SfoI 2039 GGCGCC GGC^GCC
SmaI 2942 CCCGGG CCC^GGG
SphI 4002 GCATGC GCATG^C
SspI 2798 AATATT AAT^ATT
TfiI 2987 GATTC G^A[AT]TC
XmaCI 2942 CCCGGG C^CCGGG
XmaI 2942 CCCGGG C^CCGGG
XmnI 2676 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 78 GTATAC GT^[AC][GT]AC
3444 GTCTAC
AflIII 74 ACATGT A^C[GA][CT]GT
227 ACGTGT
BbvCI 484 GCTGAGG CCTCAGC(-5/-2)
712 GCTGAGG
BciVI 2932 GGATAC GTATCC(6/5)
1265 GTATCC
BglI 3253 GCCCAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3269 GCCAGTATGGC
BlpI 2133 GCTAAGC GC^T[ACGT]AGC
4148 GCTGAGC
Bpu1102I 2133 GCTAAGC GC^T[ACGT]AGC
4148 GCTGAGC
BsaWI 1919 TCCGGA [AT]^CCGG[AT]
2990 TCCGGT
BsmBI 455 GAGACG CGTCTC(1/5)
1975 GAGACG
Bsp1407I 1184 TGTACA T^GTACA
4241 TGTACA
BsrGI 1184 TGTACA T^GTACA
4241 TGTACA
BstAPI 1717 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1747 GCAGAAGATGC
BstXI 1706 CCAGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3250 CCAGCCCAGTGG
CelII 2133 GCTAAGC GC^T[ACGT]AGC
4148 GCTGAGC
DsaI 2555 CCATGG C^C[GA][CT]GG
2762 CCATGG
HgaI 1977 GACGC GACGC(5/10)
2213 GCGTC
NcoI 2555 CCATGG C^CATGG
2762 CCATGG
SspBI 1184 TGTACA T^GTACA
4241 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PaeR7I PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII