##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
AocI Aor51HI ApaLI AscI AspI
AviII BamHI BcgI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BssSI BstBI BstEII BstPI Bsu36I
ClaI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FseI FspI KspI MluI MroI
NaeI NgoMIV NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
Psp1406I PvuI SacII SbfI SexAI
SfiI SfuI SmaI SnaBI Sse8387I
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1157 GGTACC G^GTACC
AflII 3885 CTTAAG C^TTAAG
AseI 3012 ATTAAT AT^TAAT
AsnI 3012 ATTAAT AT^TAAT
Asp700I 928 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1157 GGTACC G^GTACC
AvrII 3586 CCTAGG C^CTAGG
BclI 3773 TGATCA T^GATCA
BfrI 3885 CTTAAG C^TTAAG
BglI 1593 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3586 CCTAGG C^CTAGG
BsaBI 2128 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1003 GAATGC GAATGC(1/-1)
Bsp1407I 3468 TGTACA T^GTACA
BspHI 834 TCATGA T^CATGA
BspLU11I 3717 ACATGT A^CATGT
BsrBI 1466 GAGCGG CCGCTC(-3/-3)
BsrFI 2382 ACCGGC [GA]^CCGG[CT]
BsrGI 3468 TGTACA T^GTACA
Bst1107I 3197 GTATAC GTA^TAC
BstZ17I 3197 GTATAC GTA^TAC
Cfr10I 2382 ACCGGC [GA]^CCGG[CT]
CpoI 896 CGGACCG CG^G[AT]CCG
DraIII 3562 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3504 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3742 GATATC GAT^ATC
FbaI 3773 TGATCA T^GATCA
HindIII 496 AAGCTT A^AGCTT
HpaI 827 GTTAAC GTT^AAC
KpnI 1157 GGTACC GGTAC^C
MamI 2128 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2114 CCATGG C^CATGG
NdeI 2328 CATATG CA^TATG
PaeR7I 407 CTCGAG C^TCGAG
PshAI 3398 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3012 ATTAAT AT^TAAT
PstI 3508 CTGCAG CTGCA^G
RcaI 834 TCATGA T^CATGA
RsrII 896 CGGACCG CG^G[AT]CCG
SalI 1134 GTCGAC G^TCGAC
ScaI 597 AGTACT AGT^ACT
SgrAI 2381 CACCGGCG C[GA]^CCGG[CT]G
SpeI 1058 ACTAGT A^CTAGT
SphI 1812 GCATGC GCATG^C
SspBI 3468 TGTACA T^GTACA
XhoI 407 CTCGAG C^TCGAG
XmnI 928 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1134 GTCGAC GT^[AC][GT]AC
3197 GTATAC
AccII 26 CGCG CG^CG
28 CGCG
ApaI 1473 GGGCCC GGGCC^C
3425 GGGCCC
AvaI 407 CTCGAG C^[CT]CG[GA]G
1973 CCCGAG
BbeI 2356 GGCGCC GGCGC^C
3532 GGCGCC
BbrPI 1206 CACGTG CAC^GTG
1215 CACGTG
BlpI 2943 GCTTAGC GC^T[ACGT]AGC
3264 GCTCAGC
Bpu1102I 2943 GCTTAGC GC^T[ACGT]AGC
3264 GCTCAGC
BsaI 3485 GAGACC GGTCTC(1/5)
2247 GGTCTC
BsgI 3346 GTGCAG GTGCAG(16/14)
4215 GTGCAG
BsoBI 407 CTCGAG C^[CT]CG[GA]G
1973 CCCGAG
BssHII 25 GCGCGC G^CGCGC
27 GCGCGC
BstUI 26 CGCG CG^CG
28 CGCG
CelII 2943 GCTTAGC GC^T[ACGT]AGC
3264 GCTCAGC
DsaI 2114 CCATGG C^C[GA][CT]GG
3725 CCGTGG
FauI 1246 GCGGG CCCGC(4/6)
2631 GCGGG
HgaI 775 GACGC GACGC(5/10)
4176 GCGTC
KasI 2356 GGCGCC G^GCGCC
3532 GGCGCC
MfeI 2736 CAATTG C^AATTG
2952 CAATTG
MunI 2736 CAATTG C^AATTG
2952 CAATTG
MvnI 26 CGCG CG^CG
28 CGCG
NarI 2356 GGCGCC GG^CGCC
3532 GGCGCC
NheI 778 GCTAGC G^CTAGC
3497 GCTAGC
NspI 1812 GCATGC [GA]CATG^[CT]
3717 ACATGT
PmaCI 1206 CACGTG CAC^GTG
1215 CACGTG
PmlI 1206 CACGTG CAC^GTG
1215 CACGTG
PpuMI 2633 GGGTCCT [GA]G^G[AT]CC[CT]
3383 AGGTCCC
PvuII 986 CAGCTG CAG^CTG
3354 CAGCTG
SfoI 2356 GGCGCC GGC^GCC
3532 GGCGCC
XcmI 2011 CCAAGATGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3995 CCAAATTGTCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AhdI AluI AlwI AlwNI ApoI
AspEI AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI Eam1105I EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII