##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AclI        Aor51HI     AscI        
Asp718I     AspI        AviII       AvrII       BbrPI       
BciVI       BclI        BglII       BlnI        BsaAI       
BsiEI       Bsp1407I    BspDI       BspHI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
FbaI        FseI        FspI        HpaI        KpnI        
KspI        MfeI        MunI        NaeI        NdeI        
NgoMIV      NotI        NruI        NspV        PacI        
PflFI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        SfiI        SfuI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AgeI         3115  ACCGGT               A^CCGGT
AhdI          906  GACGTATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3138  CCTGAGG              CC^T[ACGT]AGG
AseI         2202  ATTAAT               AT^TAAT
AsnI         2202  ATTAAT               AT^TAAT
Asp700I      1995  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         906  GACGTATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1453  GGATCC               G^GATCC
BsaBI          55  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI        2889  CGTACG               C^GTACG
BsmI         1024  GAATGC               GAATGC(1/-1)
BsrBI        1052  GAGCGG               CCGCTC(-3/-3)
BstAPI        937  GCAGCAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       3138  CCTGAGG              CC^T[ACGT]AGG
DraIII       3013  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          769  GACTCTCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      906  GACGTATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3138  CCTGAGG              CC^T[ACGT]AGG
EcoRI        3236  GAATTC               G^AATTC
EcoRV        1307  GATATC               GAT^ATC
EcoT22I      2735  ATGCAT               ATGCA^T
HincII       3753  GTTGAC               GT[CT]^[GA]AC
HindII       3753  GTTGAC               GT[CT]^[GA]AC
HindIII       751  AAGCTT               A^AGCTT
MamI           55  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI           10  ACGCGT               A^CGCGT
NheI          628  GCTAGC               G^CTAGC
NsiI         2735  ATGCAT               ATGCA^T
PaeR7I        339  CTCGAG               C^TCGAG
PflMI        3639  CCATGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        3115  ACCGGT               A^CCGGT
PshAI        3266  GACTGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2202  ATTAAT               AT^TAAT
SexAI         930  ACCTGGT              A^CC[AT]GGT
SgrAI         296  CACCGGCG             C[GA]^CCGG[CT]G
SmaI         3370  CCCGGG               CCC^GGG
SphI         2972  GCATGC               GCATG^C
Van91I       3639  CCATGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          295  CCACCGGCGCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          339  CTCGAG               C^TCGAG
XmaCI        3370  CCCGGG               C^CCGGG
XmaI         3370  CCCGGG               C^CCGGG
XmnI         1995  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI         1762  GTCTAC               GT^[AC][GT]AC
             1800  GTAGAC              
AccIII        756  TCCGGA               T^CCGGA
             1368  TCCGGA              
AcsI         2070  AAATTC               [GA]^AATT[CT]
             3236  GAATTC              
AflII        1226  CTTAAG               C^TTAAG
             1676  CTTAAG              
ApoI         2070  AAATTC               [GA]^AATT[CT]
             3236  GAATTC              
BalI         1266  TGGCCA               TGG^CCA
             2771  TGGCCA              
BbeI          300  GGCGCC               GGCGC^C
              645  GGCGCC              
BbsI           80  GAAGAC               GAAGAC(2/6)
             3363  GTCTTC              
BbvCI        3507  CCTCAGC              CCTCAGC(-5/-2)
              188  GCTGAGG             
BcgI          331  GCAGGCCTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              820  GCAGCAGAGTCG        
BfrI         1226  CTTAAG               C^TTAAG
             1676  CTTAAG              
BglI          723  GCCTTGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2612  GCCTGCTGGGC         
BlpI          950  GCTGAGC              GC^T[ACGT]AGC
             1258  GCTCAGC             
Bpu1102I      950  GCTGAGC              GC^T[ACGT]AGC
             1258  GCTCAGC             
BpuAI          80  GAAGAC               GAAGAC(2/6)
             3363  GTCTTC              
BseAI         756  TCCGGA               T^CCGGA
             1368  TCCGGA              
BseRI        3564  CTCCTC               GAGGAG(10/8)
             1061  GAGGAG              
BsmBI         373  CGTCTC               CGTCTC(1/5)
             3556  GAGACG              
BspEI         756  TCCGGA               T^CCGGA
             1368  TCCGGA              
BspLU11I     2398  ACATGT               A^CATGT
             3200  ACATGT              
BspMI        1254  ACCTGC               ACCTGC(4/8)
             3785  ACCTGC              
BsrFI         297  ACCGGC               [GA]^CCGG[CT]
             3115  ACCGGT              
CelII         950  GCTGAGC              GC^T[ACGT]AGC
             1258  GCTCAGC             
Cfr10I        297  ACCGGC               [GA]^CCGG[CT]
             3115  ACCGGT              
HgaI         3326  GACGC                GACGC(5/10)
             1198  GCGTC               
KasI          300  GGCGCC               G^GCGCC
              645  GGCGCC              
MluNI        1266  TGGCCA               TGG^CCA
             2771  TGGCCA              
MroI          756  TCCGGA               T^CCGGA
             1368  TCCGGA              
MscI         1266  TGGCCA               TGG^CCA
             2771  TGGCCA              
NarI          300  GGCGCC               GG^CGCC
              645  GGCGCC              
ScaI          141  AGTACT               AGT^ACT
             1209  AGTACT              
SfoI          300  GGCGCC               GGC^GCC
              645  GGCGCC              
StuI          333  AGGCCT               AGG^CCT
             3492  AGGCCT              
TaqI          340  TCGA                 T^CGA
             1638  TCGA                
TthHB8I       340  TCGA                 T^CGA
             1638  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BsaWI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XhoII