################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AclI Aor51HI AscI Asp718I AspI AviII AvrII BbrPI BciVI BclI BglII BlnI BsaAI BsiEI Bsp1407I BspDI BspHI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoO65I FbaI FseI FspI HpaI KpnI KspI MfeI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PflFI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SfiI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AgeI 3115 ACCGGT A^CCGGT AhdI 906 GACGTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3138 CCTGAGG CC^T[ACGT]AGG AseI 2202 ATTAAT AT^TAAT AsnI 2202 ATTAAT AT^TAAT Asp700I 1995 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 906 GACGTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1453 GGATCC G^GATCC BsaBI 55 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 2889 CGTACG C^GTACG BsmI 1024 GAATGC GAATGC(1/-1) BsrBI 1052 GAGCGG CCGCTC(-3/-3) BstAPI 937 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 3138 CCTGAGG CC^T[ACGT]AGG DraIII 3013 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 769 GACTCTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 906 GACGTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3138 CCTGAGG CC^T[ACGT]AGG EcoRI 3236 GAATTC G^AATTC EcoRV 1307 GATATC GAT^ATC EcoT22I 2735 ATGCAT ATGCA^T HincII 3753 GTTGAC GT[CT]^[GA]AC HindII 3753 GTTGAC GT[CT]^[GA]AC HindIII 751 AAGCTT A^AGCTT MamI 55 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 10 ACGCGT A^CGCGT NheI 628 GCTAGC G^CTAGC NsiI 2735 ATGCAT ATGCA^T PaeR7I 339 CTCGAG C^TCGAG PflMI 3639 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 3115 ACCGGT A^CCGGT PshAI 3266 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2202 ATTAAT AT^TAAT SexAI 930 ACCTGGT A^CC[AT]GGT SgrAI 296 CACCGGCG C[GA]^CCGG[CT]G SmaI 3370 CCCGGG CCC^GGG SphI 2972 GCATGC GCATG^C Van91I 3639 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 295 CCACCGGCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 339 CTCGAG C^TCGAG XmaCI 3370 CCCGGG C^CCGGG XmaI 3370 CCCGGG C^CCGGG XmnI 1995 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 1762 GTCTAC GT^[AC][GT]AC 1800 GTAGAC AccIII 756 TCCGGA T^CCGGA 1368 TCCGGA AcsI 2070 AAATTC [GA]^AATT[CT] 3236 GAATTC AflII 1226 CTTAAG C^TTAAG 1676 CTTAAG ApoI 2070 AAATTC [GA]^AATT[CT] 3236 GAATTC BalI 1266 TGGCCA TGG^CCA 2771 TGGCCA BbeI 300 GGCGCC GGCGC^C 645 GGCGCC BbsI 80 GAAGAC GAAGAC(2/6) 3363 GTCTTC BbvCI 3507 CCTCAGC CCTCAGC(-5/-2) 188 GCTGAGG BcgI 331 GCAGGCCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 820 GCAGCAGAGTCG BfrI 1226 CTTAAG C^TTAAG 1676 CTTAAG BglI 723 GCCTTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2612 GCCTGCTGGGC BlpI 950 GCTGAGC GC^T[ACGT]AGC 1258 GCTCAGC Bpu1102I 950 GCTGAGC GC^T[ACGT]AGC 1258 GCTCAGC BpuAI 80 GAAGAC GAAGAC(2/6) 3363 GTCTTC BseAI 756 TCCGGA T^CCGGA 1368 TCCGGA BseRI 3564 CTCCTC GAGGAG(10/8) 1061 GAGGAG BsmBI 373 CGTCTC CGTCTC(1/5) 3556 GAGACG BspEI 756 TCCGGA T^CCGGA 1368 TCCGGA BspLU11I 2398 ACATGT A^CATGT 3200 ACATGT BspMI 1254 ACCTGC ACCTGC(4/8) 3785 ACCTGC BsrFI 297 ACCGGC [GA]^CCGG[CT] 3115 ACCGGT CelII 950 GCTGAGC GC^T[ACGT]AGC 1258 GCTCAGC Cfr10I 297 ACCGGC [GA]^CCGG[CT] 3115 ACCGGT HgaI 3326 GACGC GACGC(5/10) 1198 GCGTC KasI 300 GGCGCC G^GCGCC 645 GGCGCC MluNI 1266 TGGCCA TGG^CCA 2771 TGGCCA MroI 756 TCCGGA T^CCGGA 1368 TCCGGA MscI 1266 TGGCCA TGG^CCA 2771 TGGCCA NarI 300 GGCGCC GG^CGCC 645 GGCGCC ScaI 141 AGTACT AGT^ACT 1209 AGTACT SfoI 300 GGCGCC GGC^GCC 645 GGCGCC StuI 333 AGGCCT AGG^CCT 3492 AGGCCT TaqI 340 TCGA T^CGA 1638 TCGA TthHB8I 340 TCGA T^CGA 1638 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII