##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AclI Aor51HI AscI
Asp718I AspI AviII AvrII BbrPI
BciVI BclI BglII BlnI BsaAI
BsiEI Bsp1407I BspDI BspHI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
EagI EclXI Eco47III Eco52I EcoO65I
FbaI FseI FspI HpaI KpnI
KspI MfeI MunI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PflFI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SfiI SfuI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AgeI 3115 ACCGGT A^CCGGT
AhdI 906 GACGTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3138 CCTGAGG CC^T[ACGT]AGG
AseI 2202 ATTAAT AT^TAAT
AsnI 2202 ATTAAT AT^TAAT
Asp700I 1995 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 906 GACGTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1453 GGATCC G^GATCC
BsaBI 55 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 2889 CGTACG C^GTACG
BsmI 1024 GAATGC GAATGC(1/-1)
BsrBI 1052 GAGCGG CCGCTC(-3/-3)
BstAPI 937 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3138 CCTGAGG CC^T[ACGT]AGG
DraIII 3013 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 769 GACTCTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 906 GACGTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3138 CCTGAGG CC^T[ACGT]AGG
EcoRI 3236 GAATTC G^AATTC
EcoRV 1307 GATATC GAT^ATC
EcoT22I 2735 ATGCAT ATGCA^T
HincII 3753 GTTGAC GT[CT]^[GA]AC
HindII 3753 GTTGAC GT[CT]^[GA]AC
HindIII 751 AAGCTT A^AGCTT
MamI 55 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 10 ACGCGT A^CGCGT
NheI 628 GCTAGC G^CTAGC
NsiI 2735 ATGCAT ATGCA^T
PaeR7I 339 CTCGAG C^TCGAG
PflMI 3639 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3115 ACCGGT A^CCGGT
PshAI 3266 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2202 ATTAAT AT^TAAT
SexAI 930 ACCTGGT A^CC[AT]GGT
SgrAI 296 CACCGGCG C[GA]^CCGG[CT]G
SmaI 3370 CCCGGG CCC^GGG
SphI 2972 GCATGC GCATG^C
Van91I 3639 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 295 CCACCGGCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 339 CTCGAG C^TCGAG
XmaCI 3370 CCCGGG C^CCGGG
XmaI 3370 CCCGGG C^CCGGG
XmnI 1995 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1762 GTCTAC GT^[AC][GT]AC
1800 GTAGAC
AccIII 756 TCCGGA T^CCGGA
1368 TCCGGA
AcsI 2070 AAATTC [GA]^AATT[CT]
3236 GAATTC
AflII 1226 CTTAAG C^TTAAG
1676 CTTAAG
ApoI 2070 AAATTC [GA]^AATT[CT]
3236 GAATTC
BalI 1266 TGGCCA TGG^CCA
2771 TGGCCA
BbeI 300 GGCGCC GGCGC^C
645 GGCGCC
BbsI 80 GAAGAC GAAGAC(2/6)
3363 GTCTTC
BbvCI 3507 CCTCAGC CCTCAGC(-5/-2)
188 GCTGAGG
BcgI 331 GCAGGCCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
820 GCAGCAGAGTCG
BfrI 1226 CTTAAG C^TTAAG
1676 CTTAAG
BglI 723 GCCTTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2612 GCCTGCTGGGC
BlpI 950 GCTGAGC GC^T[ACGT]AGC
1258 GCTCAGC
Bpu1102I 950 GCTGAGC GC^T[ACGT]AGC
1258 GCTCAGC
BpuAI 80 GAAGAC GAAGAC(2/6)
3363 GTCTTC
BseAI 756 TCCGGA T^CCGGA
1368 TCCGGA
BseRI 3564 CTCCTC GAGGAG(10/8)
1061 GAGGAG
BsmBI 373 CGTCTC CGTCTC(1/5)
3556 GAGACG
BspEI 756 TCCGGA T^CCGGA
1368 TCCGGA
BspLU11I 2398 ACATGT A^CATGT
3200 ACATGT
BspMI 1254 ACCTGC ACCTGC(4/8)
3785 ACCTGC
BsrFI 297 ACCGGC [GA]^CCGG[CT]
3115 ACCGGT
CelII 950 GCTGAGC GC^T[ACGT]AGC
1258 GCTCAGC
Cfr10I 297 ACCGGC [GA]^CCGG[CT]
3115 ACCGGT
HgaI 3326 GACGC GACGC(5/10)
1198 GCGTC
KasI 300 GGCGCC G^GCGCC
645 GGCGCC
MluNI 1266 TGGCCA TGG^CCA
2771 TGGCCA
MroI 756 TCCGGA T^CCGGA
1368 TCCGGA
MscI 1266 TGGCCA TGG^CCA
2771 TGGCCA
NarI 300 GGCGCC GG^CGCC
645 GGCGCC
ScaI 141 AGTACT AGT^ACT
1209 AGTACT
SfoI 300 GGCGCC GGC^GCC
645 GGCGCC
StuI 333 AGGCCT AGG^CCT
3492 AGGCCT
TaqI 340 TCGA T^CGA
1638 TCGA
TthHB8I 340 TCGA T^CGA
1638 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII