##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI AseI AsnI Asp700I Asp718I
AviII BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlpI Bpu1102I
BsiWI BsmBI BspDI BspHI BsrFI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KasI KpnI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SfiI SfoI SfuI
SgrAI SnaBI SpeI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 3366 GTCGAC GT^[AC][GT]AC
AccIII 1705 TCCGGA T^CCGGA
AcsI 3598 AAATTT [GA]^AATT[CT]
AflIII 79 ACATGT A^C[GA][CT]GT
AhdI 2454 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 18 GTGCAC G^TGCAC
Aor51HI 774 AGCGCT AGC^GCT
ApaLI 18 GTGCAC G^TGCAC
ApoI 3598 AAATTT [GA]^AATT[CT]
AspEI 2454 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1243 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 614 TGGCCA TGG^CCA
BamHI 3095 GGATCC G^GATCC
BbvCI 2720 CCTCAGC CCTCAGC(-5/-2)
BglI 3657 GCCATTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1072 TACGTG [CT]AC^GT[GA]
BsaBI 1344 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1705 TCCGGA T^CCGGA
BspEI 1705 TCCGGA T^CCGGA
BspLU11I 79 ACATGT A^CATGT
BsrDI 3847 GCAATG GCAATG(2/0)
BstAPI 3064 GCATGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2361 GGTGACC G^GT[ACGT]ACC
BstPI 2361 GGTGACC G^GT[ACGT]ACC
DraI 3932 TTTAAA TTT^AAA
Eam1105I 2454 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 774 AGCGCT AGC^GCT
EcoO65I 2361 GGTGACC G^GT[ACGT]ACC
FauI 857 CCCGC CCCGC(4/6)
HincII 3366 GTCGAC GT[CT]^[GA]AC
HindII 3366 GTCGAC GT[CT]^[GA]AC
HindIII 3455 AAGCTT A^AGCTT
KspI 28 CCGCGG CCGC^GG
MamI 1344 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 614 TGGCCA TGG^CCA
MroI 1705 TCCGGA T^CCGGA
MscI 614 TGGCCA TGG^CCA
PaeR7I 1081 CTCGAG C^TCGAG
PflFI 1243 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3291 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1078 GAGCTC GAGCT^C
SacII 28 CCGCGG CCGC^GG
SalI 3366 GTCGAC G^TCGAC
SbfI 2366 CCTGCAGG CCTGCA^GG
ScaI 539 AGTACT AGT^ACT
Sse8387I 2366 CCTGCAGG CCTGCA^GG
SspI 3919 AATATT AAT^ATT
SwaI 3931 ATTTAAAT ATTT^AAAT
Tth111I 1243 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1081 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 29 CGCG CG^CG
116 CGCG
AocI 237 CCTGAGG CC^T[ACGT]AGG
2456 CCTGAGG
ApaI 1391 GGGCCC GGGCC^C
1590 GGGCCC
AvrII 2946 CCTAGG C^CTAGG
3140 CCTAGG
BlnI 2946 CCTAGG C^CTAGG
3140 CCTAGG
BsiEI 671 CGGCCG CG[GA][CT]^CG
1251 CGGCCG
BsmI 2381 GAATGC GAATGC(1/-1)
2476 GAATGC
Bsp1407I 408 TGTACA T^GTACA
1995 TGTACA
BsrBI 8 CCGCTC CCGCTC(-3/-3)
1254 CCGCTC
BsrGI 408 TGTACA T^GTACA
1995 TGTACA
BstUI 29 CGCG CG^CG
116 CGCG
BstXI 956 CCAAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1439 CCAGTCGTATGG
BstYI 1708 GGATCT [GA]^GATC[CT]
3095 GGATCC
Bsu36I 237 CCTGAGG CC^T[ACGT]AGG
2456 CCTGAGG
EagI 671 CGGCCG C^GGCCG
1251 CGGCCG
EarI 470 CTCTTC CTCTTC(1/4)
2726 CTCTTC
EclXI 671 CGGCCG C^GGCCG
1251 CGGCCG
Eco52I 671 CGGCCG C^GGCCG
1251 CGGCCG
Eco81I 237 CCTGAGG CC^T[ACGT]AGG
2456 CCTGAGG
Ksp632I 470 CTCTTC CTCTTC(1/4)
2726 CTCTTC
MflI 1708 GGATCT [GA]^GATC[CT]
3095 GGATCC
MslI 1203 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1312 CACATCAATG
MvnI 29 CGCG CG^CG
116 CGCG
SapI 469 GCTCTTC GCTCTTC(1/4)
2725 GCTCTTC
SexAI 1244 ACCAGGT A^CC[AT]GGT
1496 ACCAGGT
SmaI 387 CCCGGG CCC^GGG
983 CCCGGG
SmlI 1081 CTCGAG C^T[CT][GA]AG
3452 CTCAAG
SphI 483 GCATGC GCATG^C
831 GCATGC
SspBI 408 TGTACA T^GTACA
1995 TGTACA
StuI 1874 AGGCCT AGG^CCT
3481 AGGCCT
XcmI 1286 CCATGCGGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3874 CCACTGTCGGAGTGG
XhoII 1708 GGATCT [GA]^GATC[CT]
3095 GGATCC
XmaCI 387 CCCGGG C^CCGGG
983 CCCGGG
XmaI 387 CCCGGG C^CCGGG
983 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I