##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AclI AflII AgeI AocI
ApaI AscI Asp718I AvrII BalI
BbeI BbvCI BcgI BfrI BlnI
BsiWI BsmBI BspDI BspHI BsrBI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI Eco81I EcoRV FseI
KasI KpnI MluI MluNI MscI
NarI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I StuI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 199 GACGTC GACGT^C
AflIII 1350 ACATGT A^C[GA][CT]GT
Alw44I 3043 GTGCAC G^TGCAC
ApaLI 3043 GTGCAC G^TGCAC
AviII 3868 TGCGCA TGC^GCA
BamHI 55 GGATCC G^GATCC
BanI 4453 GGCACC G^G[CT][GA]CC
BglI 2968 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 1760 CGGCCG CG[GA][CT]^CG
BspLU11I 1350 ACATGT A^CATGT
BstEII 2431 GGTAACC G^GT[ACGT]ACC
BstPI 2431 GGTAACC G^GT[ACGT]ACC
DraIII 3212 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1760 CGGCCG C^GGCCG
EclXI 1760 CGGCCG C^GGCCG
Eco52I 1760 CGGCCG C^GGCCG
EcoO65I 2431 GGTAACC G^GT[ACGT]ACC
EcoT22I 3774 ATGCAT ATGCA^T
FspI 3868 TGCGCA TGC^GCA
KspI 179 CCGCGG CCGC^GG
MfeI 1574 CAATTG C^AATTG
MunI 1574 CAATTG C^AATTG
NaeI 2920 GCCGGC GCC^GGC
NdeI 4287 CATATG CA^TATG
NgoMIV 2920 GCCGGC G^CCGGC
NheI 486 GCTAGC G^CTAGC
NsiI 3774 ATGCAT ATGCA^T
SacI 3357 GAGCTC GAGCT^C
SacII 179 CCGCGG CCGC^GG
ScaI 1706 AGTACT AGT^ACT
SexAI 3213 ACCTGGT A^CC[AT]GGT
SmlI 4664 CTCAAG C^T[CT][GA]AG
SpeI 3163 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AhdI 2680 GACGCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2869 GACCTGCAGTC
AlwNI 405 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
442 CAGAAGCTG
AseI 2053 ATTAAT AT^TAAT
3732 ATTAAT
AsnI 2053 ATTAAT AT^TAAT
3732 ATTAAT
Asp700I 2719 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3834 GAACAATTTC
AspEI 2680 GACGCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2869 GACCTGCAGTC
AspI 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2302 GACACTGTC
BanII 3027 GGGCTC G[GA]GC[CT]^C
3357 GAGCTC
BciVI 2397 GGATAC GTATCC(6/5)
2412 GGATAC
BclI 744 TGATCA T^GATCA
1400 TGATCA
BglII 399 AGATCT A^GATCT
897 AGATCT
BlpI 2354 GCTCAGC GC^T[ACGT]AGC
3354 GCTGAGC
Bpu1102I 2354 GCTCAGC GC^T[ACGT]AGC
3354 GCTGAGC
BsaBI 1443 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1548 GATTGGAATC
Bsp1407I 2897 TGTACA T^GTACA
3851 TGTACA
BsrFI 1842 ACCGGC [GA]^CCGG[CT]
2920 GCCGGC
BsrGI 2897 TGTACA T^GTACA
3851 TGTACA
BstAPI 2180 GCACGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3045 GCACCCGCTGC
BstXI 552 CCAGCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2666 CCATCGTCCTGG
CelII 2354 GCTCAGC GC^T[ACGT]AGC
3354 GCTGAGC
Cfr10I 1842 ACCGGC [GA]^CCGG[CT]
2920 GCCGGC
DrdI 2662 GACGCCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4428 GACTGAGCAGTC
Eam1105I 2680 GACGCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2869 GACCTGCAGTC
EcoNI 31 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1927 CCTGAATCAGG
EcoRI 1129 GAATTC G^AATTC
3752 GAATTC
FbaI 744 TGATCA T^GATCA
1400 TGATCA
HindIII 631 AAGCTT A^AGCTT
710 AAGCTT
HpaI 688 GTTAAC GTT^AAC
4553 GTTAAC
MamI 1443 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1548 GATTGGAATC
NcoI 545 CCATGG C^CATGG
2436 CCATGG
PflFI 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2302 GACACTGTC
PshBI 2053 ATTAAT AT^TAAT
3732 ATTAAT
PstI 2872 CTGCAG CTGCA^G
2933 CTGCAG
SalI 1274 GTCGAC G^TCGAC
1765 GTCGAC
SapI 413 GCTCTTC GCTCTTC(1/4)
1539 GCTCTTC
SphI 2906 GCATGC GCATG^C
3014 GCATGC
SspBI 2897 TGTACA T^GTACA
3851 TGTACA
Tth111I 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2302 GACACTGTC
XmnI 2719 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3834 GAACAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AluI AlwI Aor51HI
ApoI AspHI AvaI AvaII BbrPI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspEI BspMI
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII