################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## Acc65I AclI AflII AgeI AocI ApaI AscI Asp718I AvrII BalI BbeI BbvCI BcgI BfrI BlnI BsiWI BsmBI BspDI BspHI BsrBI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI Eco81I EcoRV FseI KasI KpnI MluI MluNI MscI NarI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 199 GACGTC GACGT^C AflIII 1350 ACATGT A^C[GA][CT]GT Alw44I 3043 GTGCAC G^TGCAC ApaLI 3043 GTGCAC G^TGCAC AviII 3868 TGCGCA TGC^GCA BamHI 55 GGATCC G^GATCC BanI 4453 GGCACC G^G[CT][GA]CC BglI 2968 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiEI 1760 CGGCCG CG[GA][CT]^CG BspLU11I 1350 ACATGT A^CATGT BstEII 2431 GGTAACC G^GT[ACGT]ACC BstPI 2431 GGTAACC G^GT[ACGT]ACC DraIII 3212 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1760 CGGCCG C^GGCCG EclXI 1760 CGGCCG C^GGCCG Eco52I 1760 CGGCCG C^GGCCG EcoO65I 2431 GGTAACC G^GT[ACGT]ACC EcoT22I 3774 ATGCAT ATGCA^T FspI 3868 TGCGCA TGC^GCA KspI 179 CCGCGG CCGC^GG MfeI 1574 CAATTG C^AATTG MunI 1574 CAATTG C^AATTG NaeI 2920 GCCGGC GCC^GGC NdeI 4287 CATATG CA^TATG NgoMIV 2920 GCCGGC G^CCGGC NheI 486 GCTAGC G^CTAGC NsiI 3774 ATGCAT ATGCA^T SacI 3357 GAGCTC GAGCT^C SacII 179 CCGCGG CCGC^GG ScaI 1706 AGTACT AGT^ACT SexAI 3213 ACCTGGT A^CC[AT]GGT SmlI 4664 CTCAAG C^T[CT][GA]AG SpeI 3163 ACTAGT A^CTAGT ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AhdI 2680 GACGCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2869 GACCTGCAGTC AlwNI 405 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG 442 CAGAAGCTG AseI 2053 ATTAAT AT^TAAT 3732 ATTAAT AsnI 2053 ATTAAT AT^TAAT 3732 ATTAAT Asp700I 2719 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3834 GAACAATTTC AspEI 2680 GACGCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2869 GACCTGCAGTC AspI 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2302 GACACTGTC BanII 3027 GGGCTC G[GA]GC[CT]^C 3357 GAGCTC BciVI 2397 GGATAC GTATCC(6/5) 2412 GGATAC BclI 744 TGATCA T^GATCA 1400 TGATCA BglII 399 AGATCT A^GATCT 897 AGATCT BlpI 2354 GCTCAGC GC^T[ACGT]AGC 3354 GCTGAGC Bpu1102I 2354 GCTCAGC GC^T[ACGT]AGC 3354 GCTGAGC BsaBI 1443 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1548 GATTGGAATC Bsp1407I 2897 TGTACA T^GTACA 3851 TGTACA BsrFI 1842 ACCGGC [GA]^CCGG[CT] 2920 GCCGGC BsrGI 2897 TGTACA T^GTACA 3851 TGTACA BstAPI 2180 GCACGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3045 GCACCCGCTGC BstXI 552 CCAGCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2666 CCATCGTCCTGG CelII 2354 GCTCAGC GC^T[ACGT]AGC 3354 GCTGAGC Cfr10I 1842 ACCGGC [GA]^CCGG[CT] 2920 GCCGGC DrdI 2662 GACGCCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4428 GACTGAGCAGTC Eam1105I 2680 GACGCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2869 GACCTGCAGTC EcoNI 31 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1927 CCTGAATCAGG EcoRI 1129 GAATTC G^AATTC 3752 GAATTC FbaI 744 TGATCA T^GATCA 1400 TGATCA HindIII 631 AAGCTT A^AGCTT 710 AAGCTT HpaI 688 GTTAAC GTT^AAC 4553 GTTAAC MamI 1443 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1548 GATTGGAATC NcoI 545 CCATGG C^CATGG 2436 CCATGG PflFI 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2302 GACACTGTC PshBI 2053 ATTAAT AT^TAAT 3732 ATTAAT PstI 2872 CTGCAG CTGCA^G 2933 CTGCAG SalI 1274 GTCGAC G^TCGAC 1765 GTCGAC SapI 413 GCTCTTC GCTCTTC(1/4) 1539 GCTCTTC SphI 2906 GCATGC GCATG^C 3014 GCATGC SspBI 2897 TGTACA T^GTACA 3851 TGTACA Tth111I 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2302 GACACTGTC XmnI 2719 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3834 GAACAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AcyI AfaI AluI AlwI Aor51HI ApoI AspHI AvaI AvaII BbrPI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspEI BspMI BsrDI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII