##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AviII AvrII BamHI BbeI
BbrPI BcgI BclI BfrI BglII
BlnI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrDI BsrFI BssHII BstAPI BstBI
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FbaI FseI FspI HgaI HindIII
HpaI KasI KspI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI TfiI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 2339 GACGTC GACGT^C
Acc65I 3043 GGTACC G^GTACC
AcyI 2339 GACGTC G[GA]^CG[CT]C
AflIII 543 ACATGT A^C[GA][CT]GT
Alw44I 592 GTGCAC G^TGCAC
AocI 2453 CCTCAGG CC^T[ACGT]AGG
ApaI 1025 GGGCCC GGGCC^C
ApaLI 592 GTGCAC G^TGCAC
Asp718I 3043 GGTACC G^GTACC
BalI 953 TGGCCA TGG^CCA
BglI 1018 GCCACAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3110 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3110 GCTGAGC GC^T[ACGT]AGC
BsaHI 2339 GACGTC G[GA]^CG[CT]C
BsaWI 2987 TCCGGT [AT]^CCGG[AT]
BsgI 3373 GTGCAG GTGCAG(16/14)
BsmI 3387 GAATGC GAATGC(1/-1)
Bsp1407I 546 TGTACA T^GTACA
BspHI 1280 TCATGA T^CATGA
BspLU11I 543 ACATGT A^CATGT
BspMI 23 GCAGGT ACCTGC(4/8)
BsrGI 546 TGTACA T^GTACA
Bst1107I 2842 GTATAC GTA^TAC
BstXI 1503 CCAGATACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2842 GTATAC GTA^TAC
Bsu36I 2453 CCTCAGG CC^T[ACGT]AGG
CelII 3110 GCTGAGC GC^T[ACGT]AGC
DrdI 3315 GACAGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2453 CCTCAGG CC^T[ACGT]AGG
EcoNI 1086 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1963 ATGCAT ATGCA^T
HaeII 2947 GGCGCT [GA]GCGC^[CT]
Hin1I 2339 GACGTC G[GA]^CG[CT]C
HincII 3058 GTTGAC GT[CT]^[GA]AC
HindII 3058 GTTGAC GT[CT]^[GA]AC
KpnI 3043 GGTACC GGTAC^C
MfeI 769 CAATTG C^AATTG
MluNI 953 TGGCCA TGG^CCA
MscI 953 TGGCCA TGG^CCA
MunI 769 CAATTG C^AATTG
NcoI 932 CCATGG C^CATGG
NheI 992 GCTAGC G^CTAGC
NsiI 1963 ATGCAT ATGCA^T
RcaI 1280 TCATGA T^CATGA
SapI 2926 GCTCTTC GCTCTTC(1/4)
SexAI 1844 ACCAGGT A^CC[AT]GGT
SfiI 1017 GGCCACAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2907 CCCGGG CCC^GGG
SspBI 546 TGTACA T^GTACA
XmaCI 2907 CCCGGG C^CCGGG
XmaI 2907 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 650 AAATTC [GA]^AATT[CT]
2268 AAATTT
ApoI 650 AAATTC [GA]^AATT[CT]
2268 AAATTT
AspI 1843 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3282 GACAAGGTC
BciVI 1332 GGATAC GTATCC(6/5)
1921 GGATAC
DraIII 1397 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2073 CACTTGGTG
DsaI 932 CCATGG C^C[GA][CT]GG
1265 CCGTGG
EaeI 187 TGGCCG [CT]^GGCC[GA]
953 TGGCCA
MslI 550 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1615 CATGGGGGTG
PaeR7I 2220 CTCGAG C^TCGAG
2474 CTCGAG
PflFI 1843 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3282 GACAAGGTC
PflMI 256 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2042 CCACCACATGG
PstI 1177 CTGCAG CTGCA^G
1562 CTGCAG
ScaI 1876 AGTACT AGT^ACT
1895 AGTACT
SphI 1122 GCATGC GCATG^C
1228 GCATGC
Tth111I 1843 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3282 GACAAGGTC
Van91I 256 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2042 CCACCACATGG
XhoI 2220 CTCGAG C^TCGAG
2474 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII